Anti-freezing composition comprising gold nanoparticle with peptide

Patent metadata

Field	Value
Publication number	US-11849720-B2
Application number	US-201916702059-A
Country	US
Kind code	B2
Filing date	Dec 3, 2019
Priority date	Oct 15, 2019
Publication date	Dec 26, 2023
Grant date	Dec 26, 2023

Abstract

The present invention provides a a nanostructure which includes: a core comprising one or more planes configured to come into planar contact with at least one plane of an ice crystal; and an oligopeptide which is conjugated to at least one plane of the core and comprises (Thr)n-, (Ala)n-, (Ser)n-, or (Gly)n-, wherein the nanostructure is a polyhedron-shaped nanostructure present in water in a colloidal form to control freezing, and n is an integer of 2 to 7.

First claim

What is claimed is:

1. A nanostructure comprising:
a core comprising one or more planes configured to come into planar contact with at least one plane of an ice crystal; and
an oligopeptide which is conjugated to at least one plane of the core and comprises (Thr) n -, (Ala) n -, (Ser) n -, or (Gly) n -,
wherein the core has a polyhedron shape, and the nanostructure is a polyhedron-shaped nanostructure present in water in a colloidal form to control freezing, and
n is an integer of 2 to 7,
wherein the nanostructure has a higher ice recrystallization inhibition (IRI) activity than the same nanostructure but in a sphere-shaped structure.

2. The nanostructure according to claim 1 , wherein the polyhedron shape comprises tetrahedron, truncated tetrahedron, hexahedron, truncated hexahedron, octahedron, truncated octahedron, decahedron, dodecahedron, icosahedron, tetrakishexahedron, hexakisoctahedron, or rhombic dodecahedron.

3. The nanostructure according to claim 1 , wherein the core is made of gold (Au), silver (Ag), platinum (Pt), palladium (Pd), aluminum (Al), copper (Cu), iron oxide (Fe 3 O 4 ), or silicon dioxide (SiO 2 ), or an alloy including at least two thereof.

4. The nanostructure according to claim 1 , wherein the core is made of gold (Au), platinum (Pt), iron (Fe), iron oxide (Fe 3 O 4 ), silicon (Si), or silicon dioxide (SiO 2 ), or an alloy including at least two thereof.

5. The nanostructure according to claim 1 , wherein n is 3 to 6.

6. The nanostructure according to claim 1 , wherein the oligopeptide comprises (Thr) n -, or (Ala) n -.

7. The nanostructure according to claim 1 , wherein the core has a diameter of 50 nm to 100 nm.

8. The nanostructure according to claim 1 , wherein 0.07 to 0.25 oligopeptides per nm 2 are attached to the plane.

9. The nanostructure according to claim 1 , wherein the core has a shape of a hexahedron or octahedron.

10. The nanostructure according to claim 1 , wherein the core is made of gold (Au).

11. The nanostructure according to claim 1 , wherein n is 5.

12. The nanostructure according to claim 1 , wherein 0.1 to 0.2 oligopeptides per nm 2 are attached to the plane.

13. The nanostructure according to claim 1 , wherein the freezing control is performed by inhibiting recrystallization of the ice.

14. A composition for controlling freezing comprising the nanostructure according to claim 1 .

15. A composition for freezing a cell comprising the nanostructure according to claim 1 .

16. A composition for freezing a food comprising the nanostructure according to claim 1 .

17. A method for cryopreserving a cell comprising: adding the nanostructure according to claim 1 , to a solution containing the cells.

18. A method for cryopreserving a food comprising: adding the nanostructure according to claim 1 , to the food for treatment.

Assignees

• Univ Korea Res & Bus Found

Inventors

• Lee Seungwoo
• Ahn Dong June
• Lim Dong Kwon
• Lee Jae Won
• Lee Sang Yup

Classifications

• A01N1/125 Primary

  Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators · CPC title

• A23B2/85

  with addition of {or treatment with} chemicals · CPC title

• A01N1/0221 Primary

  Human Necessities · mapped topic

• A23L3/37

  Human Necessities · mapped topic

• C07K19/00

  Hybrid peptides {, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes} · CPC title