Full continuous-flow preparation method of L-carnitine

Patent metadata

Field	Value
Publication number	US-11827581-B2
Application number	US-202217741112-A
Country	US
Kind code	B2
Filing date	May 10, 2022
Priority date	May 10, 2022
Publication date	Nov 28, 2023
Grant date	Nov 28, 2023

Abstract

A full continuous-flow preparation method of L-carnitine, including: mixing chlorine gas and a diketene solution via a first micromixer followed by transportation to a first microchannel reactor for continuous chlorination and esterification reaction to obtain 4-chloroacetoacetate; feeding the 4-chloroacetoacetate and a reductase to a second micromixer and a second microchannel reactor in sequence for continuous catalytic reaction to obtain (R)-4-chloro-3-hydroxybutyrate; simultaneously transporting the (R)-4-chloro-3-hydroxybutyrate and a trimethylamine solution to a third micromixer and a third microchannel reactor for continuous substitution and hydrolysis reaction; and subjecting the reaction mixture to desalination and concentration to obtain the L-carnitine.

First claim

What is claimed is:

1. A full continuous-flow preparation method of L-carnitine using a micro-reaction system, the micro-reaction system comprising a first micromixer, a first microchannel reactor, a second micromixer, a second microchannel reactor, a third micromixer, a third microchannel reactor, a fourth micromixer and a fourth microchannel reactor communicated in sequence; and the method comprising:
(S1) respectively transporting chlorine gas and a diketene reaction liquid to the first micromixer for mixing, and allowing the reaction mixture in the first micromixer to flow into the first microchannel reactor followed by a continuous chlorination reaction;
(S2) transporting the reaction mixture flowing out from the first microchannel reactor and an alcohol solvent into the second micromixer and the second microchannel reactor in sequence fora continuous esterification reaction;
(S3) neutralizing the reaction mixture flowing out from the second microchannel reactor with a first alkali followed by a continuous extraction and separation to collect a first organic phase; simultaneously transporting the first organic phase and an aqueous solution of a reductase to the third micromixer and the third microchannel reactor fora continuous reduction reaction;
(S4) removing the reductase from the reaction mixture flowing out from the third microchannel reactor via an extraction separator followed by a continuous extraction and separation to obtain a second organic phase; and concentrating the second organic phase to obtain a concentrated liquid;
(S5) simultaneously transporting the concentrated liquid obtained in step (S4) and a trimethylamine solution to the fourth micromixer and the fourth microchannel reactor in sequence fora continuous substitution and hydrolysis reaction; and
(S6) neutralizing the reaction mixture flowing out from the fourth microchannel reactor with a second alkali followed by feeding the neutralized reaction mixture into a demineralization device and a concentration device to obtain the L-carnitine.

2. The full continuous-flow preparation method of claim 1 , wherein the diketene reaction liquid is a solution of diketene in an organic solvent; the organic solvent is selected from the group consisting of benzene, methylbenzene, an ester solvent, a chlorinated hydrocarbon solvent and an alkane solvent;
the reductase is a carbonyl reductase; and
the trimethylamine solution is a trimethylamine aqueous solution or a trimethylamine alcoholic solution.

3. The full continuous-flow preparation method of claim 1 , wherein in step (S1), a flow ratio of the chlorine gas to the diketene reaction liquid is controlled such that a molar ratio of diketene to the chlorine gas is 1:0.9-1.5;
the first micromixer and the first microchannel reactor are both controlled at −20˜50° C.; a residence time of the reaction mixture in the first microchannel reactor is 0.1˜30 min; and a back pressure during the continuous chlorination reaction is controlled to 0.1˜1.0 MPa.

4. The full continuous-flow preparation method of claim 1 , wherein in step (S2), the alcohol solvent is ethanol; a flow ratio of the reaction mixture obtained in step (S1) to ethanol is controlled such that a molar ratio of initial diketene to ethanol is 1:0.9-1.5; the second micromixer and the second microchannel reactor are both controlled at 20-80° C.; a residence time of the reaction mixture in the second microchannel reactor is 0.1-30 min; and a back pressure during the continuous esterification reaction is controlled to 0.1-1.0 MPa.

5. The full continuous-flow preparation method of claim 1 , wherein in step (S3), the third micromixer and the third microchannel reactor are both controlled at 20-50° C.; a residence time of the reaction mixture in the third microchannel reactor is 1-30 min; and a back pressure during the continuous reduction reaction is controlled to 0.1-1.0 MPa.

6. The full continuous-flow preparation method of claim 1 , wherein in step (S5), a flow ratio of the concentrated liquid obtained in step (S4) to the trimethylamine solution is controlled such that a molar ratio of initial diketene to trimethylamine is 1:1.0-2.0; the fourth micromixer and the fourth microchannel reactor are both controlled at 20-50° C.; a residence time of the reaction mixture in the fourth microchannel reactor is 1-30 min; and a back pressure during the continuous substitution and hydrolysis reaction is 0.1-1.0 MPa.

7. The full continuous-flow preparation method of claim 1 , wherein the first micromixer, the second micromixer, the third micromixer and the fourth micromixer are each independently selected from the group consisting of static mixer, T-type micromixer, Y-type micromixer, cross-type mixer, coaxial flow micromixer and flow-focusing micromixer; and the first microchannel reactor, the second microchannel reactor, the third microchannel reactor and the fourth microchannel reactor are each independently a tubular microchannel reactor, a plate-type microchannel reactor, or other commercially available microchannel reactors.

8. The full continuous-flow preparation method of claim 7 , wherein an inner diameter of the tubular microchannel reactor is 50 μm-10 mm; the plate-type microchannel reactor comprises a first heat exchange layer, a reaction layer and a second heat exchange layer arranged in sequence from top to bottom; the reaction layer is provided with a reaction fluid channel; and a hydraulic diameter of the reaction fluid channel is 50 μm-10 mm.

9. The full continuous-flow preparation method of claim 1 , wherein the continuous extraction separator is a plate-type microchannel extraction separator, a membrane extraction separator, an annular centrifugal extraction separator, a ceramic membrane separator, an electrodialysis device, a continuous concentration device, or a multi-effect falling-film evaporator.

Assignees

• Univ Fudan

Inventors

• Chen Fener
• Liu Minjie
• Jiang Meifen
• Cheng Dang
• Yu Chao
• Huang Huashan

Classifications

• C07C227/12 Primary

  Formation of amino and carboxyl groups · CPC title

• B01J19/0093

  Microreactors, e.g. miniaturised or microfabricated reactors (laboratory containers with capillary fluid transport in microfabricated channels or chambers B01L3/5027) · CPC title

• C07C227/08 Primary

  by reaction of ammonia or amines with acids containing functional groups · CPC title

• B01J2219/0086

  Dimensions of the flow channels · CPC title

• B01J2219/00867

  Microreactors placed in series, on the same or on different supports · CPC title