Integrated biocontainment cell sorter

Patent metadata

Field	Value
Publication number	US-11808689-B2
Application number	US-202217692234-A
Country	US
Kind code	B2
Filing date	Mar 11, 2022
Priority date	Jun 26, 2019
Publication date	Nov 7, 2023
Grant date	Nov 7, 2023

Abstract

Disclosed is an integrated biocontainment cell sorter that isolates portions of the cell sorter that can create contamination. Two containment systems are utilized. A main cabinet containment system contains input samples. An aerosol management containment area includes a nozzle chamber with a nozzle and a sort chamber with sort plates and collection media that collect a droplet stream from the nozzle. The main cabinet is maintained at a first low pressure and clean air is recirculated under a positive pressure. The aerosol management containment area is kept at a second low pressure, which is lower than the first pressure, so that contamination does not leak from the aerosol management containment area into the main cabinet containment area. A sliding sash window is located over an access opening in the main cabinet and can be moved to access different portions of the main cabinet without changing the substantially constant first low pressure in the main cabinet.

First claim

The invention claimed is:

1. A biocontainment system, comprising:
a main cabinet, the main cabinet enclosing a work area therein;
a first fan train,
the first fan train comprising a first fan and a first filter,
the first fan being operable to draw air from the main cabinet through the first filter;

an aerosol management system,
the aerosol management system comprising a first chamber,
the first chamber being located within the main cabinet and being in fluid communication with the work area of the main cabinet,

the aerosol management system comprising a second fan train,
the second fan train comprising a second fan and a second filter,

the second fan being operable to draw air from the first chamber through the second filter and to maintain the first chamber at a pressure lower than a pressure of the work area.

2. The biocontainment system of claim 1 , wherein the aerosol management system comprises a second chamber, the second chamber being located within the main cabinet and the second chamber being in fluid communication with the work area of the main cabinet.

3. The biocontainment system of claim 2 , wherein the second fan is operable to draw air from the second chamber through the second filter and to maintain the second chamber at a pressure lower than a pressure of the work area.

4. The biocontainment system of claim 2 , comprising a passage between the first chamber and the second chamber.

5. The biocontainment system of claim 1 , wherein the aerosol management system comprises a recirculation duct, the recirculation duct being configured to receive air from the first fan train.

6. The biocontainment system of claim 5 , further comprising a recirculation plenum configured to communicate air from the recycle duct to the work area.

7. The biocontainment system of claim 5 , further comprising an exhaust vent configured to receive air from the first fan train and communicate the air to the environment exterior to the main cabinet.

8. The biocontainment system of claim 7 , wherein the second fan train is in fluid communication with the exhaust vent.

9. The biocontainment system of claim 1 , wherein the main cabinet comprises an opening configured to place the main cabinet into fluid communication with the environment exterior to the main.

10. The biocontainment system of claim 1 , further comprising an optical system configured to interrogate a sample residing within the aerosol management system.

11. The biocontainment system of claim 10 , wherein a portion of the optical system is located outside of the first chamber.

12. The biocontainment system of claim 1 , further comprising a particle sorter, the particle sorter located within the aerosol management system.

13. A method of containing material in a biocontainment system, comprising:
operating a first fan to move air through a first flow path,
the first flow path being defined by (i) a work area within a main cabinet, (ii) a first filter, and (iii) a recirculation duct in fluid communication with the work area and with a first exhaust vent; and

operating a second fan to move air through an aerosol management system of the biocontainment system,
the aerosol management system comprising (i) the second fan, (ii) a first chamber in communication with the work area, and (iii) a second filter that receives air communicated from the first chamber, the aerosol management system being arranged such that the work area is free of air filtered by the second filter; and

maintaining the first chamber at a lower pressure than the main cabinet.

14. The method of claim 13 , wherein the second flow path comprises a second chamber, the second chamber being maintained at a lower pressure than the main cabinet.

15. The method of claim 13 , further comprising sorting a plurality of particles located within the second flow path.

16. The method of claim 15 , further comprising optically exciting the plurality of particles.

17. The method of claim 13 , wherein the second flow path comprises an exhaust vent.

18. A biocontainment system, comprising:
a main cabinet having a work area therein;
a first chamber having a particle processing train located therein,
the first chamber being disposed within the main cabinet,
the first chamber being in fluid communication with the work area; and

a filtration train,
the filtration train being configured to (i) collect and filter air from the work area, (ii) collect and filter air from the first chamber, and (iii) maintain the first chamber at a pressure lower than a pressure of the work area.

19. The biocontainment system of claim 18 , wherein the particle processing train comprises a particle sorter.

20. The biocontainment system of claim 18 , wherein the filtration train is configured to return filtered air to the work area.

Assignees

• Life Technologies Corp

Inventors

• Gulley Lincoln T
• Fox Daniel N
• Goldfain Angela L

Classifications

• G01N15/01

  specially adapted for biological cells, e.g. blood cells (investigating sedimentation of particle suspensions in blood G01N15/05) · CPC title

• G01N15/1434

  Optical arrangements · CPC title

• G01N15/1409

  Handling samples, e.g. injecting samples · CPC title

• G01N15/1492 Primary

  within droplets · CPC title

• G01N15/1459 Primary

  the analysis being performed on a sample stream · CPC title