Slider tape sealing cartridge for adjustably sealing a flow cytometer sample manipulation chamber

Patent metadata

Field	Value
Publication number	US-9575050-B2
Application number	US-201514686367-A
Country	US
Kind code	B2
Filing date	Apr 14, 2015
Priority date	Apr 21, 2014
Publication date	Feb 21, 2017
Grant date	Feb 21, 2017

Abstract

Flow cytometer systems that include a sample manipulation chamber having an access hole that is adjustably sealed by a slider tape sealing cartridge are provided. Also provided are methods of using such systems, as well as components for use in such systems.

First claim

What is claimed is:

1. A flow cytometer comprising:
(a) a sample manipulation chamber comprising an access hole;
(b) a sealing cartridge adjustably sealing the access hole of the sample manipulation chamber, wherein the sealing cartridge comprises:
(i) a slider tape comprising an access hole, and
(ii) a first winder at one end of the slider tape, configured to reversibly wind the slider tape; and

(c) a mobile arm penetrating the access holes of the slider tape and the sample manipulation chamber.

2. The flow cytometer according to claim 1 , wherein the mobile arm is configured to manipulate samples in the sample manipulation chamber.

3. The flow cytometer according to claim 1 , wherein the flow cytometer comprises a sample collection vessel within the sample manipulation chamber.

4. The flow cytometer according to claim 3 , wherein the mobile arm is configured to manipulate the sample collection vessel.

5. The flow cytometer according to claim 1 , wherein the sample manipulation chamber comprises an influx opening and an efflux opening, configured so that fluid flows into the sample manipulation chamber through the influx opening and out of the sample manipulation chamber through the efflux opening.

6. The flow cytometer according to claim 1 , further comprising an air filtration system fluidically coupled with the sample manipulation chamber.

7. The flow cytometer according to claim 1 , wherein the flow cytometer comprises an excitation light source.

8. The flow cytometer according to claim 1 , wherein the flow cytometer comprises a detector.

9. The flow cytometer according to claim 1 , wherein the flow cytometer comprises a deflection plate.

10. The flow cytometer according to claim 1 , wherein the sealing cartridge comprises a first winder at one end of the slider tape and a second winder at the other end of the slider tape, wherein the first and second winders are each configured to reversibly wind the slider tape.

11. The flow cytometer according to claim 1 , wherein the sealing cartridge comprises a front plate and a back plate attached to one another and enclosing the slider tape.

12. A method of performing a flow cytometric procedure, the method comprising:
(a) introducing a sample into a flow cytometer comprising:
(i) a sample manipulation chamber comprising and an access hole;
(ii) a sealing cartridge adjustably sealing the access hole of the sample manipulation chamber, wherein the sealing cartridge comprises:
(a) a slider tape comprising an access hole, and
(b) a winder at one end of the slider tape, configured to reversibly wind the slider tape; and

(iii) a mobile arm penetrating the access holes of the slider tape and the sample manipulation chamber; and

(b) manipulating with the mobile arm a sample collection vessel within the sample manipulation chamber.

13. The method according to claim 12 , wherein the flow cytometer comprises an air filtration system fluidically coupled with the sample manipulation chamber.

14. The method according to claim 13 , further comprising actuating the air filtration system.

15. The method according to claim 12 , further comprising flow cytometrically analyzing particles of the sample.

16. The method according to claim 15 , wherein flow cytometrically analyzing comprises sorting particles of the sample into one or more sample collection vessels.

17. The method according to claim 16 , wherein sorting particles of the sample includes the manipulating of step (b).

18. A sample manipulation system comprising:
(i) a sample manipulation chamber comprising an access hole; and
(ii) a sealing cartridge adjustably sealing the access hole of the sample manipulation chamber, wherein the sealing cartridge comprises:
(a) a slider tape comprising an access hole, and
(b) a first winder at one end of the slider tape, configured to reversibly wind the slider tape.

19. The sample manipulation system according to claim 18 , wherein the sample manipulation chamber comprises an influx opening and an efflux opening, configured so that fluid flows into the sample manipulation chamber through the influx opening and out of the sample manipulation chamber through the efflux opening.

20. The sample manipulation system according to claim 18 , wherein the sealing cartridge comprises a first winder at one end of the slider tape and a second winder at the other end of the slider tape, wherein the first and second winders are each configured to reversibly wind the slider tape.

Assignees

• Becton Dickinson Co

Inventors

• Vrane David
• Norton Pierce O

Classifications

• G01N33/4833 Primary

  of solid biological material, e.g. tissue samples, cell cultures (tissue in vivo A61B5/00; cell suspensions G01N33/48735) · CPC title

• G01N15/14 Primary

  Optical investigation techniques, e.g. flow cytometry · CPC title

• G01N35/0099

  comprising robots or similar manipulators (robots per se B25J) · CPC title