On-chip nanoscale storage system using chimeric DNA

Patent metadata

Field	Value
Publication number	US-11755922-B2
Application number	US-201916593450-A
Country	US
Kind code	B2
Filing date	Oct 4, 2019
Priority date	Oct 4, 2019
Publication date	Sep 12, 2023
Grant date	Sep 12, 2023

Abstract

The present disclosure provides systems and methods that can provide portable, real-time accessible DNA memories. An example DNA-based data storage system includes a loading region configured to receive a plurality of DNA-based data storage elements in a suspension fluid and a plurality of microtubes disposed in a capture/release region. The microtubes are configured to capture and release the DNA-based data storage elements. The DNA-based data storage system also includes a linearization region configured to linearize the DNA-based data storage elements and a readout region with a readout device configured to provide information indicative of the respective DNA-based data storage elements.

First claim

What is claimed is:

1. A deoxyribonucleic acid (DNA)-based data storage element comprising:
a DNA backbone, and multiple non-natural nucleic acids bioconjugated to the DNA backbone, wherein the multiple non-natural nucleic acids are arranged in a plurality of blocks, wherein each block corresponds to an alphabet symbol of an alphabet, wherein the alphabet comprises at least n 4 alphabet symbols, where n is greater than 1, wherein at least a portion of the blocks comprise nonuniform length repetition patterns;
wherein each of the multiple non-natural nucleic acids independently comprise a triazole moiety linked to a non-natural functional group.

2. The DNA-based data storage element of claim 1 , wherein at least one of the non-natural nucleic acids comprise a peptide nucleic acid (PNA).

3. The DNA-based data storage element of claim 2 , wherein the PNA comprises a peptide backbone and a plurality of natural nucleobase monomers.

4. The DNA-based data storage element of claim 1 , wherein the DNA backbone comprises single-stranded DNA.

5. The DNA-based data storage element of claim 1 , wherein the DNA backbone comprises double-stranded DNA.

6. The DNA-based data storage element of claim 1 , wherein the plurality of non-natural nucleic acids comprises a structurally-defined branched polymer architecture.

7. The DNA-based data storage element of claim 1 , wherein the multiple non-natural nucleic acids do not comprise a nucleobase moiety.

8. The DNA-based data storage element of claim 1 , wherein the triazole moiety linked to a non-natural functional group is in place of a nucleobase.

9. The DNA-based data storage element of claim 1 , wherein the DNA backbone further comprises at least one abasic site.

10. The DNA-based data storage element of claim 1 , wherein the multiple non-natural nucleic acids are distinguishable by a DNA sequencer.

11. The DNA-based data storage element of claim 1 , wherein the non-natural nucleic acids are configured to encode information.

12. A microfluidic deoxyribonucleic acid (DNA)-based data storage system, comprising:
a suspension fluid comprising a plurality of the DNA-based data storage elements of claim 1 ;
a loading region configured to receive the plurality of DNA-based data storage elements in a suspension fluid;
a plurality of microtubes disposed in a capture/release region, wherein the microtubes are configured to capture and release the DNA-based data storage elements;
a linearization region configured to linearize the DNA-based data storage elements; and
a readout region with a readout device configured to provide information indicative of the respective DNA-based data storage elements.

13. The DNA-based data storage system of claim 12 , wherein at least one microtube of the plurality of microtubes comprises a self-rolled microtube.

14. The DNA-based data storage system of claim 13 , wherein, in an initial condition, the self-rolled microtube comprises:
a substrate;
a sacrificial etch material overlaying the substrate;
a compressive layer overlaying the sacrificial etch material;
a tensile layer overlaying the compressive layer; and
a plurality of electrodes.

15. The DNA-based data storage system of claim 14 , wherein, in a rolled condition, the self-rolled microtube comprises:
at least a portion of the tensile and compressive layers rolled into a tubular shape having a diameter of less than 10 microns.

16. The DNA-based data storage system of claim 12 , wherein the readout device comprises a solid-state nanopore device.

17. The DNA-based data storage system of claim 12 , wherein the readout device comprises a tandem mass spectrometry system.

18. A method to synthesize the deoxyribonucleic acid (DNA)-based data storage element of claim 1 , comprising:
selecting an abasic site of a DNA backbone;
modifying the abasic site to be compatible with bioconjugation by way of cycloaddition; and
performing a bioconjugation so as to add at least one non-natural functional group to the abasic site as modified.

19. The method of claim 18 , wherein the bioconjugation comprises an azide-alkyne Huisgen-type cycloaddition.

20. The method of claim 18 , wherein modifying the abasic site is performed so as to form a bioconjugation click chemistry target.

Assignees

• Univ Illinois

Inventors

• Milenkovic Olgica
• Athreya Nagendra
• Khandelwal Apratim
• Leburton Jean-Pierre
• Li Xiuling
• Schroeder Charles
• Tabatabaei Seyedkasra
• Li Bo

Classifications

• C12N7/00 Primary

  Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00) · CPC title

• G06N3/123 Primary

  DNA computing · CPC title

• C12N2795/14131

  Uses of virus other than therapeutic or vaccine, e.g. disinfectant · CPC title

• C12N2795/14142

  virus or viral particle as vehicle, e.g. encapsulating small organic molecule · CPC title

• C40B40/06

  Libraries containing nucleotides or polynucleotides, or derivatives thereof · CPC title