HSD17B13 variants and uses thereof

Patent metadata

Field	Value
Publication number	US-11753628-B2
Application number	US-202217709965-A
Country	US
Kind code	B2
Filing date	Mar 31, 2022
Priority date	Jan 23, 2017
Publication date	Sep 12, 2023
Grant date	Sep 12, 2023

Abstract

Provided are compositions related to HSD17B13 variants, including nucleic acid molecules and polypeptides related to variants of HSD17B13, and cells comprising those nucleic acid molecules and polypeptides. Also provided are methods related to HSD17B13 variants. Such methods include methods for detecting the presence of the HSD17B13 rs72613567 variant in a biological sample comprising genomic DNA, for detecting the presence or levels of any one of variant HSD17B13 Transcripts C, D, E, F, G, and H, and particularly D, in a biological sample comprising mRNA or cDNA, or for detecting the presence or levels of any one of variant HSD17B13 protein Isoforms C, D, E, F, G, or H, and particularly D, in a biological sample comprising protein. Also provided are methods for determining a subject's susceptibility to developing a liver disease or of diagnosing a subject with liver disease.

First claim

What is claimed is:

1. A method of treating a subject who is not a carrier of the HSD17B13 rs72613567 variant and has a chronic liver disease, the method comprising introducing into the subject an antisense molecule that hybridizes to an HSD17B13 nucleic acid molecule and decreases expression of HSD17B13 in a liver cell in the subject.

2. The method of claim 1 , wherein the antisense molecule hybridizes to a sequence within exon 7 or a sequence spanning the exon 6-exon 7 boundary of SEQ ID NO:12 (HSD17B13 Transcript A) and decreases expression of HSD17B13 Transcript A in a liver cell in the subject.

3. The method of claim 2 , wherein the antisense molecule comprises an antisense RNA that hybridizes to a sequence within exon 7 of SEQ ID NO:12 (HSD17B13 Transcript A) is introduced into the subject.

4. The method of claim 2 , wherein the antisense molecule comprises an antisense RNA that hybridizes to a sequence spanning the exon 6-exon 7 boundary of SEQ ID NO:12 (HSD17B13 Transcript A) is introduced into the subject.

5. The method of claim 2 , wherein the antisense molecule comprises an siRNA that hybridizes to a sequence within exon 7 of SEQ ID NO:12 (HSD17B13 Transcript A) is introduced into the subject.

6. The method of claim 2 , wherein the antisense molecule comprises an siRNA that hybridizes to a sequence spanning the exon 6-exon 7 boundary of SEQ ID NO:12 (HSD17B13 Transcript A) is introduced into the subject.

7. The method of claim 2 , wherein the antisense molecule comprises an shRNA that hybridizes to a sequence within exon 7 of SEQ ID NO:12 (HSD17B13 Transcript A) is introduced into the subject.

8. The method of claim 2 , wherein the antisense molecule comprises an shRNA that hybridizes to a sequence spanning the exon 6-exon 7 boundary of SEQ ID NO:12 (HSD17B13 Transcript A) is introduced into the subject.

9. The method of claim 1 , wherein the subject is a human.

10. The method of claim 1 , wherein the chronic liver disease is nonalcoholic fatty liver disease (NAFLD).

11. The method of claim 1 , wherein the chronic liver disease is alcoholic liver fatty liver disease.

12. The method of claim 1 , wherein the chronic liver disease is cirrhosis.

13. The method of claim 1 , wherein the chronic liver disease is hepatocellular carcinoma.

14. A method of treating a subject who has at least one risk factor for progression to more clinically advanced stages of liver disease, the method comprising introducing into the subject an antisense molecule that hybridizes to an HSD17B13 nucleic acid molecule and decreases expression of HSD17B13 in a liver cell in the subject, wherein the at least one risk factor comprising the subject not being a carrier of a HSD17B13 rs72613567 variant, excessive alcohol use, obesity, high cholesterol, a high level of triglycerides in the subject's blood, polycystic ovary syndrome, sleep apnea, type 2 diabetes, underactive thyroid (hypothyroidism), underactive pituitary gland (hypopituitarism), and/or a metabolic syndrome including raised blood lipids.

15. The method of claim 14 , wherein the subject has an increased risk of histopathological progression from simple steatosis to steatohepatitis.

16. The method of claim 14 , wherein the subject has an increased risk of histopathological progression from simple steatosis to fibrosis.

17. The method of claim 14 , wherein the subject has an increased risk of histopathological progression from simple steatosis to cirrhosis.

18. The method of claim 14 , wherein the subject has an increased risk of histopathological progression from simple steatosis to hepatocellular carcinoma.

19. The method of claim 14 , wherein the antisense molecule hybridizes to a sequence within exon 7 or a sequence spanning the exon 6-exon 7 boundary of SEQ ID NO:12 (HSD17B13 Transcript A) and decreases expression of HSD17B13 Transcript A in a liver cell in the subject.

20. The method of claim 19 , wherein the antisense molecule comprises an antisense RNA that hybridizes to a sequence within exon 7 of SEQ ID NO:12 (HSD17B13 Transcript A) is introduced into the subject.

21. The method of claim 19 , wherein the antisense molecule comprises an antisense RNA that hybridizes to a sequence spanning the exon 6-exon 7 boundary of SEQ ID NO:12 (HSD17B13 Transcript A) is introduced into the subject.

22. The method of claim 19 , wherein the antisense molecule comprises an siRNA that hybridizes to a sequence within exon 7 of SEQ ID NO:12 (HSD17B13 Transcript A) is introduced into the subject.

23. The method of claim 19 , wherein the antisense molecule comprises an siRNA that hybridizes to a sequence spanning the exon 6-exon 7 boundary of SEQ ID NO: 12 (HSD17B13 Transcript A) is introduced into the subject.

24. The method of claim 19 , wherein the antisense molecule comprises an shRNA that hybridizes to a sequence within exon 7 of SEQ ID NO:12 (HSD17B13 Transcript A) is introduced into the subject.

25. The method of claim 19 , wherein the antisense molecule comprises an shRNA that hybridizes to a sequence spanning the exon 6-exon 7 boundary of SEQ ID NO:12 (HSD17B13 Transcript A) is introduced into the subject.

26. The method of claim 14 , wherein the subject is a human.

Assignees

• Regeneron Pharma

Inventors

• Abul-Husn Noura S
• Gottesman Omri
• Li Alexander
• Cheng Xiping
• Xin Yurong
• Gromada Jesper
• Dewey Frederick E
• Baras Aris
• Shuldiner Alan

Classifications

• C12N15/907

  in mammalian cells · CPC title

• C12N9/22

  Ribonucleases {[RNase]; Deoxyribonucleases [DNase]} · CPC title

• C12N2310/531

  Stem-loop; Hairpin · CPC title

• C12N2310/20

  involving clustered regularly interspaced short palindromic repeats [CRISPR] · CPC title

• C12N2310/14

  interfering nucleic acids [NA] · CPC title