Droplet dispensing

Patent metadata

Field	Value
Publication number	US-11740255-B2
Application number	US-201816616864-A
Country	US
Kind code	B2
Filing date	Nov 9, 2018
Priority date	Nov 14, 2017
Publication date	Aug 29, 2023
Grant date	Aug 29, 2023

Abstract

A system includes a droplet actuator having a droplet-operation gap between top and bottom substrates, a reservoir(s) external to and coupled to the droplet actuator, the reservoir(s) sized for a large-volume fluid, and pressure source(s) external to the droplet actuator and coupled to the at least one reservoir. Operation of the system includes filling the reservoir(s) with a large volume of fluid(s), dispensing droplet(s) of the fluid(s) to the droplet-operation gap using the pressure source(s) as part of performing a droplet operation(s). Movement of the droplet(s) may be effectuated by activating the droplet actuator.

First claim

The invention claimed is:

1. A system, comprising:
a droplet actuator having a top substrate, a bottom substrate, and a droplet-operation gap between the top and bottom substrates;
at least one reservoir external to and coupled to the droplet actuator, the at least one reservoir being sized for a large-volume fluid;
at least one pressure source external to the droplet actuator and coupled to the at least one reservoir such that the at least one pressure source is configured to pressurize the large-volume fluid in the at least one reservoir and drive the pressurized large-volume fluid from the at least one reservoir to the droplet-operation gap via one or more loading ports formed in the droplet actuator; and
a spacer positioned in the droplet-operation gap adjacent an inlet of the droplet actuator to shrink the inlet vertically and/or horizontally.

2. The system of claim 1 , wherein the at least one pressure source comprises a constant pressure source.

3. The system of claim 1 , wherein the at least one pressure source comprises at least one of one or more positive pressure sources and one or more negative pressure sources.

4. The system of claim 1 , wherein one or more reservoirs of the at least one reservoir are connected to the droplet actuator, and wherein a seal for at least one of the one or more reservoirs comprises a male seal portion and a female seal portion, one of the male seal portion and the female seal portion being located at a bottom of the droplet actuator, and the other of the male seal portion and the female seal portion being coupled to the at least one of the one or more reservoirs.

5. The system of claim 1 , further comprising at least one conduit coupling the droplet actuator and the at least one reservoir, wherein a seal is located at the droplet actuator.

6. The system of claim 1 , wherein a removed portion of the top substrate creates an abrupt increase in a height of the droplet-operation gap at side edges of the top substrate corresponding to the removed portion.

7. The system of claim 1 , wherein the droplet actuator further comprises at least one inlet for fluid, the system further comprising at least one valve coupled to the at least one inlet to control a flow of one or more fluids incoming to the droplet actuator.

8. The system of claim 1 , wherein the one or more loading ports are formed in the top substrate or the bottom substrate.

9. The system of claim 1 , wherein the at least one reservoir is coupled to the droplet actuator via a connector.

10. The system of claim 9 , wherein the connector comprises a tube, a sipper, or a locking tip.

11. The system of claim 1 , wherein the top substrate or the bottom substrate includes at least one stepped portion, such that the droplet-operation gap has a first height at a first location along the droplet actuator and a second height at a second location along the droplet actuator, the second height being different from the first height.

12. A method, comprising:
filling at least one reservoir of a system with a large volume of one or more fluids, wherein the system further comprises:
a droplet actuator having a top substrate, a bottom substrate, and a droplet-operation gap between the top and bottom substrates;
at least one pressure source external to the droplet actuator and coupled to the at least one reservoir, and wherein the at least one reservoir is externally coupled to the droplet actuator; and
a spacer positioned in the droplet-operation gap adjacent an inlet of the droplet actuator to shrink the inlet vertically and/or horizontally;

pressurizing the one or more fluids in the at least one reservoir using the at least one pressure source; and
dispensing at least one droplet of the pressurized one or more fluids to the droplet-operation gap using the at least one pressure source as part of performing at least one droplet operation.

13. The method of claim 12 , wherein the at least one pressure source comprises one or more positive pressure sources, and wherein the dispensing comprises pushing the at least one droplet from one or more of the at least one reservoir to the droplet-operation gap.

14. The method of claim 12 , wherein the at least one pressure source comprises one or more constant pressure sources, and wherein the dispensing comprises using the one or more constant pressure sources to dispense the at least one droplet.

15. The method of claim 12 , wherein a removed portion of the top substrate creates an abrupt increase in a height of the droplet-operation gap at side edges of the top substrate corresponding to the removed portion, wherein the droplet-operation gap comprises a gap under the removed portion situated between the top substrate and the bottom substrate, and wherein the dispensing comprises dispensing the at least one droplet to the droplet-operation gap outside the gap under the removed portion.

16. The method of claim 12 , wherein one or more pressure sources of the at least one pressure source and one or more reservoirs of the at least one reservoir are bottom-coupled to the droplet actuator, and wherein the dispensing comprises dispensing the at least one droplet to the droplet-operation gap using the one or more pressure sources and the one or more reservoirs.

17. The method of claim 16 , wherein the at least one pressure source further comprises one or more pressure sources side-coupled to the droplet actuator, and wherein the dispensing comprises dispensing the at least one droplet to the droplet-operation gap also using the one or more pressure sources side-coupled to the droplet actuator.

18. The method of claim 12 , wherein the at least one pressure source comprises one or more pressure sources top-coupled to the droplet actuator, and wherein the dispensing comprises dispensing the at least one droplet to the droplet-operation gap using the one or more pressure sources top-coupled to the droplet actuator.

19. The method of claim 12 , wherein the droplet actuator comprises an electrowetting-based droplet actuator, wherein a plurality of electrodes are situated in the bottom substrate and at least one electrode is associated with the top substrate, the plurality of electrodes comprising at least one array of at least two electrodes, and wherein the dispensing comprises activating the plurality of electrodes and dispensing at least two droplets onto the bottom substrate over the array.

20. A method, comprising:
coupling a droplet actuator to at least one reservoir externally coupled to the droplet actuator and sized for a large-volume fluid, the droplet actuator having a top substrate, a bottom substrate, and a droplet-operation gap between the top and bottom substrates;
coupling at least one pressure source to the droplet actuator, the at least one pressure source external to the at least one reservoir;
filling one or more of the at least one reservoir with the large-volume fluid;
activating the droplet actuator;
pressurizing the large-volume fluid in the at least one reservoir using the at least one pressure source;
dispensing, after the activating, at least one droplet of the pressurized large-volume fluid to an inlet of the droplet-operation gap of the droplet actuator using pressure provided by the at least one pressure source, the dispensing comprising controlling a flow of the large-volume fluid into the inlet using at least one valve coupled to the inlet, wherein the dispensing includes monitoring one or more aspects of the dispensing;
moving the at least one droplet along the droplet-operat

Assignees

• Illumina Inc

Inventors

• Foley Jennifer Olivia
• Segale Darren
• Delattre Cyril

Classifications

• G01N35/1016 Primary

  Control of the volume dispensed or introduced · CPC title

• B01L3/0265

  using valves to interrupt or meter fluid flow, e.g. using solenoids or metering valves · CPC title

• B01L2200/0689

  Sealing · CPC title

• B01L2400/0427

  Electrowetting · CPC title

• B01L2400/0622

  distribution valves, valves having multiple inlets and/or outlets, e.g. metering valves, multi-way valves · CPC title