Techniques and droplet actuator designs for reducing bubble formation

Patent metadata

Field	Value
Publication number	US-9238222-B2
Application number	US-201414549123-A
Country	US
Kind code	B2
Filing date	Nov 20, 2014
Priority date	Jun 27, 2012
Publication date	Jan 19, 2016
Grant date	Jan 19, 2016

Abstract

During droplet operations in a droplet actuator, bubbles often form in the filler fluid in the droplet operations gap and interrupt droplet operations. The present invention provides methods and systems for performing droplet operations on a droplet in a droplet actuator comprising maintaining substantially consistent contact between the droplet and an electrical ground while conducting multiple droplet operations on the droplet in the droplet operations gap and/or reducing the accumulation of electrical charges in the droplet operations gap during multiple droplet operations. The methods and systems reduce or eliminate bubble formation in the filler fluid of the droplet operations gap, thereby permitting completion of multiple droplet operations without interruption by bubble formation in the filler fluid in the droplet operations gap.

First claim

We claim:

1. A droplet actuator comprising:
a. a top substrate and a bottom substrate separated to form a droplet operations gap, wherein the droplet operations gap is filled with a filler fluid;
b. a sidewall and an opposite sidewall bounding the droplet operations gap, thereby creating a droplet operations channel;
c. an arrangement of droplet operations electrodes on the sidewall; and
d. an arrangement of one or more ground electrodes along the opposite sidewall, wherein the one or more ground electrodes are connected to an electrical ground;

wherein multiple droplet operations may be conducted on one or more droplets in the droplet operations gap while maintaining substantially consistent contact between the one or more droplets and the one or more ground electrodes, thereby permitting completion of the multiple droplet operations without interruption by bubble formation in the filler fluid in the droplet operations gap.

2. The droplet actuator according to claim 1 , wherein the sidewall comprises a first rail and the opposite sidewall comprises a second rail, wherein the first rail and second rail are elongated three-dimensional (3D) structures that are arranged in parallel with each other.

3. The droplet actuator according to claim 1 , further comprising offsetting positions of the droplet operations electrodes and the position of the one or more ground electrodes.

4. The droplet actuator according to claim 1 , wherein the one or more ground electrodes comprise a continuous strip.

5. The droplet actuator according to claim 1 , further comprising having droplet operations electrode arranged oppositely to each one or more ground electrode.

6. A droplet actuator comprising:
a. a top substrate and a bottom substrate separated to form a droplet operations gap, wherein the droplet operations gap is filled with a filler fluid;
b. a sidewall and an opposite sidewall bounding the droplet operations gap, thereby creating a droplet operations channel;
c. an arrangement of one or more ground electrodes along the sidewall and/or the opposite sidewall, wherein the one or more ground electrodes are connected to an electrical ground;
d. an arrangement of droplet operations electrodes on the bottom substrate,

wherein multiple droplet operations may be conducted on one or more droplets in the droplet operations gap while maintaining substantially consistent contact between the one or more droplets and the one or more ground electrodes, thereby permitting completion of the multiple droplet operations without interruption by bubble formation in the filler fluid in the droplet operations gap.

7. The droplet actuator according to claim 6 , wherein the sidewall comprises a first rail and the opposite sidewall comprises a second rail, wherein the first rail and second rail are elongated three-dimensional (3D) structures that are arranged in parallel with each other.

8. A method of performing droplet operations on a droplet in a droplet actuator, comprising:
a. providing a droplet actuator comprising:
i. a top substrate and a bottom substrate separated to form a droplet operations gap;
ii. a sidewall and an opposite sidewall bounding the droplet operations gap, thereby creating a droplet operations channel;
iii. an arrangement of droplet operations electrodes on one of the sidewall or bottom substrate; and
iv. an arrangement of one or more ground electrodes along one or more of the side wall and the opposite sidewall, wherein the one or more ground electrodes are connected to an electrical ground;

b. filling the droplet operations gap of the droplet actuator with a filler fluid;
c. providing a droplet in the droplet operations gap;
d. conducting multiple droplet operations on the droplet in the droplet operations gap, wherein the droplet is transported through the filler fluid in the droplet operations gap; and
e. maintaining substantially consistent contact between the droplet and an electrical ground while conducting the multiple droplet operations on the droplet in the droplet operations gap;

wherein the substantially consistent contact between the droplet and the electrical ground permits completion of the multiple droplet operations without interruption by bubble formation in the filler fluid in the droplet operations gap.

9. The method according to claim 8 , wherein the droplet operations electrodes are arranged on the sidewall and the one or more ground electrodes are arranged along the opposite sidewall.

10. The method according to claim 8 , wherein the droplet operations electrodes are arranged on the bottom substrate and the one or more ground electrodes are arranged along one or both of the sidewall and the opposite sidewall.

11. The method according to claim 8 , wherein the sidewall comprises a first rail and the opposite sidewall comprises a second rail, wherein the first rail and second rail are elongated three-dimensional (3D) structures that are arranged in parallel with each other.

12. The method according to claim 8 , further comprising offsetting positions of the droplet operations electrodes and the position of the one or more ground electrodes.

13. The method according to claim 8 , wherein the one or more ground electrodes comprise a continuous strip.

14. The method according to claim 8 , further comprising oppositely arranging each droplet operations electrode to each one or more ground electrode.

15. A method of performing droplet operations on a droplet in a droplet actuator, comprising:
a. providing a droplet actuator comprising:
i. a top substrate and a bottom substrate separated to form a droplet operations gap;
ii. a sidewall and an opposite sidewall bounding the droplet operations gap, thereby creating a droplet operations channel;
iii. an arrangement of droplet operations electrodes on one of the sidewall or bottom substrate; and
iv. an arrangement of one or more ground electrodes along one or more of the side wall and the opposite sidewall, wherein the one or more ground electrodes are connected to an electrical ground;

b. filling the droplet operations gap of the droplet actuator with a filler fluid;
c. providing a droplet in the droplet operations gap;
d. heating the droplet to within twenty degrees Celsius of boiling to produce a heated droplet;
e. conducting multiple droplet operations on the heated droplet in the droplet operations gap, wherein the heated droplet is transported through the filler fluid in the droplet operations gap; and
f. reducing accumulation of electrical charges in the droplet operations gap as the heated droplet is transported through the filler fluid in the droplet operations gap;

wherein the reduced accumulation of electrical charges in the droplet operations gap permits completion of the multiple droplet operations without interruption by bubble formation in the filler fluid in the droplet operations gap.

16. The method according to claim 15 , wherein the droplet operations electrodes are arranged on the sidewall and the one or more ground electrodes are arranged along the opposite sidewall.

17. The method according to claim 15 , wherein the droplet operations electrodes are arranged on the bottom substrate and the one or more ground electrodes are arranged along one or both of the sidewall and the opposite sidewall.

Assignees

• Advanced Liquid Logic Inc
• Illumina France Sarl

Inventors

• Delattre Cyril
• Rival Arnaud
• Srinivasan Vijay

Classifications

• B01L2300/0816

  Cards, e.g. flat sample carriers usually with flow in two horizontal directions · CPC title

• B01L3/502784 Primary

  specially adapted for droplet or plug flow, e.g. digital microfluidics · CPC title

• B01L2200/0605

  Metering of fluids · CPC title

• B01L3/5029

  using swabs · CPC title

• B01L2400/0415

  electrical forces, e.g. electrokinetic · CPC title