Amylases and glucoamylases, nucleic acids encoding them and methods for making and using them

Patent metadata

Field	Value
Publication number	US-11739312-B2
Application number	US-202017110222-A
Country	US
Kind code	B2
Filing date	Dec 2, 2020
Priority date	Dec 21, 2006
Publication date	Aug 29, 2023
Grant date	Aug 29, 2023

Abstract

In one aspect, the invention is directed to polypeptides having an amylase and/or glucoamylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of polysaccharide, oligosaccharide or starch into sugars. In one aspect, the invention provides delayed release compositions comprising an desired ingredient coated by a latex polymer coating. In alternative embodiments, enzymes are used to make biofuels, e.g., ethanol, butanol, propanol, or a gasoline-ethanol mix, including a bioethanol, biopropanol, biobutanol, or a biodiesel, or for any form of fuel or biomass processing.

First claim

What is claimed is:

1. An isolated, synthetic, or recombinant polypeptide comprising: an amino acid sequence having at least 99% sequence identity to SEQ ID No: 52 and a heterologous sequence, wherein the polypeptide has an alpha-amylase activity.

2. A vector, an expression cassette, or a cloning vehicle comprising:
(a) a nucleic acid comprising a nucleic acid sequence encoding the polypeptide of claim 1 ;
(b) the vector, expression cassette, or cloning vehicle of (a), wherein the nucleic acid is operably linked to a promoter;
(c) the vector, expression cassette, or cloning vehicle of (b), wherein the promoter is a plant promoter or a viral promoter; or
(d) the vector, expression cassette or cloning vehicle of (c), wherein the plant promoter is a corn seed embryo-specific promoter; a corn seed endosperm-specific promoter; or a rice seed endosperm-specific promoter.

3. A transformed host cell comprising a nucleic acid encoding the polypeptide of claim 1 .

4. A plant cell or an isolated host cell comprising a nucleic acid comprising the nucleic acid sequence of claim 2 .

5. A method for hydrolyzing a polysaccharide, oligosaccharide or starch comprising the following steps:
(a) providing the polypeptide of claim 1 ;
(b) providing a composition comprising a polysaccharide, oligosaccharide or starch; and
(c) contacting the polypeptide of step (a) with the composition of step (b) under conditions wherein the polypeptide hydrolyzes the polysaccharide, oligosaccharide or starch.

6. A method for liquefying or removing a polysaccharide, oligosaccharide or starch comprising the following steps:
(a) providing the polypeptide of claim 1 ;
(b) providing a composition comprising a polysaccharide, oligosaccharide or starch; and
(c) contacting the polypeptide of step (a) with the composition of step (b) under conditions wherein the polypeptide liquefies or removes the polysaccharide, oligosaccharide or starch.

7. A method for washing an object comprising the following steps:
(a) providing a composition comprising the polypeptide of claim 1 ;
(b) providing an object; and
(c) contacting the polypeptide of step (a) and the object of step (b) under conditions wherein the composition can wash the object.

8. A method for hydrolyzing a polysaccharide, oligosaccharide or starch in a feed or a food prior to consumption by an animal comprising the following steps:
(a) obtaining a feed material comprising a starch, where polysaccharide, oligosaccharide or starch in the polysaccharide, oligosaccharide or starch can be hydrolyzed by the polypeptide of claim 1 ; and
(b) adding the polypeptide of claim 1 to the feed or food material in an amount sufficient for a sufficient time period to cause hydrolysis of the polysaccharide, oligosaccharide or starch and formation of a treated food or feed, thereby hydrolyzing the polysaccharide, oligosaccharide or starch in the food or the feed prior to consumption by the animal.

9. A method for textile desizing comprising the following steps:
(a) providing the polypeptide of claim 1 ;
(b) providing a fabric; and
(c) contacting the polypeptide of step (a) and the fabric of step (b) under conditions wherein the alpha-amylase can desize the fabric.

10. A method for treatment of lignocellulosic fibers comprising the following steps:
(a) providing the polypeptide of claim 1 ;
(b) providing a lignocellulosic fiber; and
(c) contacting the polypeptide of step (a) and the fiber of step (b) under conditions wherein the polypeptide can treat the fiber thereby improving the fiber properties.

11. A method for producing a high-maltose or a high-glucose syrup comprising the following steps:
(a) providing the polypeptide of claim 1 ;
(b) providing a composition comprising a polysaccharide, oligosaccharide or starch; and
(c) contacting the polypeptide of step (a) and the composition of step (b) under conditions wherein the polypeptide of step (a) can hydrolyze the composition of step (b), thereby producing a high-maltose or a high-glucose syrup.

12. A method for improving the flow of the polysaccharide, oligosaccharide or starch-containing production fluids, comprising the following steps:
(a) providing the polypeptide of claim 1 ;
(b) providing production fluid comprising a polysaccharide, oligosaccharide or starch; and
(c) contacting the polypeptide of step (a) and the production fluid of step (b) under conditions wherein the polypeptide can hydrolyze the polysaccharide, oligosaccharide or starch in the production fluid, thereby improving its flow by decreasing its density.

13. A method for changing the viscosity of a composition, comprising the following steps:
(i)
(a) providing a composition and the polypeptide of claim 1 ; and
(b) treating the composition with the polypeptide of claim 1 ; or

(ii) the method of (i), wherein the composition comprises a soil or a drilling mud.

14. A method for using an alpha-amylase in brewing or alcohol production comprising
the following steps:
(a) providing the polypeptide of claim 1 ;
(b) providing a composition used for brewing or in alcohol production comprising a polysaccharide, oligosaccharide or starch;
(c) combining the polypeptide of step (a) with the composition of the step (b) under conditions wherein the polypeptide can hydrolyze the polysaccharide, oligosaccharide or starch in the composition used for brewing or alcohol production.

15. A method for bio-bleaching a composition comprising:
(i) providing a composition wherein the composition is a paper or a pulp product, and the polypeptide of claim 1 ;
(ii) treating the composition with the polypeptide of claim 1 .

16. A method for deinking of paper and fibers comprising the following steps:
(a) providing the polypeptide as set forth in claim 1 ;
(b) providing a composition comprising paper or fiber;
(c) contacting the polypeptide of step (a) and the composition of step (b) under conditions wherein the polypeptide can deink the paper or fiber.

17. A method for making a fuel comprising:
(i)
(a) providing the polypeptide of claim 1 ;
(b) providing a composition comprising a polysaccharide, oligosaccharide or starch; and
(c) contacting the polypeptide of (a) with the composition of (b) under conditions wherein the polypeptide hydrolyzes the polysaccharide, oligosaccharide or starch;

(ii) the method of (i) wherein the polypeptide is a thermostable enzyme; or
(iii) the method of (i) wherein the fuel is ethanol-based.

18. A method for processing a biomass material comprising lignocellulose comprising
(i)
(a) providing a composition comprising the polypeptide of claim 1 , and, providing a biomass material; and
(b) contacting the composition with the biomass material;

(ii) the method of (i), wherein the biomass material comprises or is obtained from an agricultural crop, or is a byproduct of a food or a feed production, or is a lignocellulosic waste product, or is a plant residue or a waste paper or waste paper product;
(iii) the method of (i) or (ii), wherein the polypeptide has activity comprising an alpha-amylase activity;
(iv) the method of any of (ii), wherein the plant residue comprise stems, leaves, hulls, husks, cobs, wood, wood chips, wood pulp and sawdust, or, the paper waste comprises discarded or used photocopy paper, computer printer paper, notebook paper, notepad paper, typewriter paper, newspapers, magazines, cardboard and paper-based packaging materials;
(v) the method of (i) to (iv), wherein the processing of the biomass material generates a bioethanol, biopropano

Assignees

• Basf Enzymes Llc
• Syngenta Participations Ag

Inventors

• Slupska Malgorzata
• Hazlewood Geoff
• Chang Cathy
• Luginbuhl Peter
• Burke Ellen
• Cayouette Michelle
• Gunawardena Uvini
• Ghassemian Majid
• Silverstone Aron
• Zhang Yan

Classifications

• C12N9/96 Primary

  Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates · CPC title

• C12N9/2408

  acting on alpha -1,4-glucosidic bonds · CPC title

• C12N9/2414

  Alpha-amylase (3.2.1.1.) · CPC title

• C12N9/2425

  Beta-amylase (3.2.1.2) · CPC title

• C12N9/2428

  Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase · CPC title