Compositions and methods for characterizing and diagnosing periodontal disease

Patent metadata

Field	Value
Publication number	US-10760109-B2
Application number	US-201515316537-A
Country	US
Kind code	B2
Filing date	Jun 8, 2015
Priority date	Jun 6, 2014
Publication date	Sep 1, 2020
Grant date	Sep 1, 2020

Abstract

The present disclosure relates to methods for characterizing and diagnosing dental diseases. In particular, the present disclosure relates to methods for characterizing and diagnosing dental disease (e.g., gingivitis and periodontal disease) based on levels of AI-2 in oral fluids and plaque.

First claim

The invention claimed is:

1. A method of diagnosing periodontal disease, comprising;
a) detecting: a1) an altered level of Autoinducer-2 (AI-2) in an oral sample; and a2) the presence of one or more bacteria in said oral sample, wherein said bacteria are one or more species selected from the group consisting of Akkermansia muciniphila, Coprobacter fastidiosus, Blautia schinkii, Bergeyella sp. AF14, and Ruminococcus gnavus , and
b) diagnosing the presence of periodontal disease when said level of AI-2, and said bacteria are altered relative to the level in subjects not diagnosed with periodontal disease.

2. The method of claim 1 , wherein said oral sample is an oral fluid or a plaque sample.

3. The method of claim 2 , wherein said oral fluid is saliva or gingival crevicular fluid (GCF).

4. The method of claim 1 , wherein said oral sample is plaque and said bacteria are one or more species selected from the group consisting of Akkermansia muciniphila, Coprobacter fastidiosus, Blautia schinkii and Ruminococcus gnavus.

5. The method of claim 1 , wherein said oral sample is saliva and said bacteria are one or more species selected from the group consisting of Akkermansia muciniphila, Bergeyella sp. AF14, and Ruminococcus gnavus.

6. The method of claim 1 , wherein said one or more species are 5 or more species.

7. The method of claim 1 , wherein said one or more species are all of said species.

8. The method of claim 1 , further comprising the step of administering a treatment for periodontal disease when said altered level of AI-2 are detected.

9. The method of claim 8 , wherein said treatment is selected from an antibiotic, a dental cleaning, and a laser treatment.

10. The method of claim 8 , wherein said treatment is in a mouthwash or toothpaste.

11. The method of claim 1 , wherein said levels of AI-2 are increased or decreased relative to the level in subjects not diagnosed with periodontal disease.

12. The method of claim 1 , wherein compounds that alter the levels of AI-2 or the presence of said bacteria in said sample relative to the levels in the absence of the compounds are identified as therapeutic agents.

13. The method of claim 1 , wherein said oral sample is saliva or and said bacteria are Akkermansia muciniphila, Ruminococcus gnavus or a combination thereof.

Assignees

• Univ Michigan Regents
• Colgate Palmolive Co

Inventors

• Rickard Alexander
• Giannobile William
• Swetky Michelle
• Baxter Nielson T
• Miller Steven E
• Du-Thumm Laurence
• Stettler Hans

Classifications

• C12Q1/18 Primary

  Testing for antimicrobial activity of a material · CPC title

• G01N33/56955

  involved in periodontal diseases · CPC title

• Y02A90/10

  Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation · CPC title

• G01N2500/00

  Screening for compounds of potential therapeutic value · CPC title

• G01N2333/195

  from bacteria · CPC title