Microfluidic chip and droplet separation method

Patent metadata

Field	Value
Publication number	US-11701659-B2
Application number	US-202117355869-A
Country	US
Kind code	B2
Filing date	Jun 23, 2021
Priority date	Nov 27, 2020
Publication date	Jul 18, 2023
Grant date	Jul 18, 2023

Abstract

The present disclosure provides a microfluidic chip and a droplet separation method, and belongs to the field of biological chip technology. The microfluidic chip includes a first liquid tank and a second liquid tank opposite to each other and a channel layer therebetween. The channel layer includes a plurality of microfluidic channels separated from each other, first ends of the microfluidic channels are communicated with the first liquid tank, and second ends are communicated with the second liquid tank. The first liquid tank contains sample solution to be detected, and the second liquid tank contains encapsulating liquid. The sample solution to be detected entering the first liquid tank may be separated into a plurality of sample droplets through the microfluidic channels, the separated sample droplets enter the second liquid tank, so that the encapsulating liquid is encapsulated on a surface of each of the plurality of sample droplets.

First claim

What is claimed is:

1. A microfluidic chip, comprising: a first liquid tank and a second liquid tank opposite to each other and a channel layer between the first liquid tank and the second liquid tank;
the channel layer comprises a plurality of microfluidic channels which are separated from each other, first ends of the microfluidic channels are communicated with the first liquid tank, and second ends of the microfluidic channels are communicated with the second liquid tank; the first liquid tank is configured to contain sample solution to be detected, and the second liquid tank is configured to contain encapsulating liquid;
the plurality of microfluidic channels are configured to separate the sample solution to be detected entering the first liquid tank into a plurality of sample droplets, and make the plurality of sample droplets enter the second liquid tank such that each of the plurality of sample droplets is encapsulated by the encapsulating liquid.

2. The microfluidic chip according to claim 1 , wherein each of the plurality of microfluidic channels comprises a first channel and a second channel which are connected to each other, the first channel is closer to the first liquid tank than the second channel; the second channel has a proximal end proximal to the first channel, and a distal end distal to the first channel; wherein,
an aperture of the distal end is larger than that of the proximal end, and an aperture of the second channel is gradually increased along a direction from the first channel to the second channel, and the aperture of the distal end of the second channel is larger than a diameter of the sample droplet.

3. The microfluidic chip according to claim 2 , wherein an aperture of the first channel is constant in the direction from the first channel to the second channel, and the aperture of the first channel is smaller than the diameter of the sample droplet.

4. The microfluidic chip according to claim 2 , wherein an orthographic projection of the first channel on a plane where the first liquid tank is located is within an orthographic projection of the second channel on the plane where the first liquid tank is located.

5. The microfluidic chip according to claim 2 , wherein an orthographic projection of the first channel on a plane where the first liquid tank is located is circular, and an orthographic projection of the second channel on the plane where the first liquid tank is located is circular.

6. The microfluidic chip according to claim 1 , wherein the plurality of microfluidic channels extend in a first direction; a plane where the first liquid tank is located is parallel to a plane where the second liquid tank is located; and the first direction is perpendicular to an extending direction of the plane where the first liquid tank is located.

7. The microfluidic chip according to claim 1 , wherein the first liquid tank has a first fluid inlet and a first fluid outlet; the second liquid tank has a second liquid inlet and a second liquid outlet; wherein,
an included angle between an extending direction of a first connection line between the first liquid inlet and the first liquid outlet and an extending direction of a second connection line between the second liquid inlet and the second liquid outlet is smaller than 90 degrees.

8. The microfluidic chip according to claim 7 , wherein the extending direction of the first connection line is parallel to the extending direction of the second connection line.

9. The microfluidic chip according to claim 1 , wherein the first liquid tank has a first fluid inlet and a first fluid outlet; the second liquid tank has a second liquid inlet and a second liquid outlet;
the microfluidic chip further comprises: a first driving device and a second driving device; the first driving device is at the first liquid inlet and is configured to drive the sample solution to be detected to flow; the second driving device is at the second liquid inlet and is configured to drive the encapsulating liquid to flow.

10. The microfluidic chip according to claim 9 , wherein the first driving device is any one of a pneumatic pump, a plunger pump, and a peristaltic pump; and/or
the second driving device is any one of a pneumatic pump, a plunger pump, and a peristaltic pump.

11. The microfluidic chip according to claim 1 , further comprising a lyophobic layer on an inner wall of each of the plurality of microfluidic channels and configured to prevent the sample solution to be detected from adhering to the inner wall.

12. The microfluidic chip according to claim 11 , wherein a material of the lyophobic layer comprises a lyophobic group and a reactive group which are connected to each other; the lyophobic group comprises an alkane having a carbon number of not less than 6; the reactive group comprises at least one of silane, siloxane, oxysilane.

13. The microfluidic chip according to claim 1 , wherein a material of the channel layer comprises at least one of silicon, glass, polymethyl methacrylate, and polycarbonate.

14. The microfluidic chip according to claim 1 , wherein a bottom surface of the second liquid tank at a side distal to the channel layer is made of a transparent material.

15. A droplet separation method by using a microfluidic chip, wherein the microfluidic chip comprises: a first liquid tank and a second liquid tank opposite to each other and a channel layer between the first liquid tank and the second liquid tank; the channel layer comprises a plurality of microfluidic channels which are separated from each other, first ends of the microfluidic channels are communicated with the first liquid tank, and second ends of the microfluidic channels are communicated with the second liquid tank; the first liquid tank is configured to contain sample solution to be detected, and the second liquid tank is configured to contain encapsulating liquid;
the droplet separation method comprises enabling the sample solution to be detected entering the first liquid tank to be separated into a plurality of sample droplets through the plurality of microfluidic channels; causing the plurality of sample droplets to enter the second liquid tank; and causing a surface of each of the plurality of sample droplets to be encapsulated by the encapsulating liquid.

16. The droplet separation method according to claim 15 , wherein a density of the sample solution to be detected is greater than that of the encapsulating liquid, and each of the plurality of microfluidic channels comprises a first channel and a second channel which are connected to each other, the first channel is closer to the first liquid tank than the second channel; the second channel has a proximal end proximal to the first channel, and a distal end distal to the first channel, and
the droplet separation method comprises steps of:
causing the first liquid tank to be below the second liquid tank;
opening a second liquid inlet and a second liquid outlet of the second liquid tank, such that the encapsulating liquid flows into the second liquid tank; and
opening a first liquid inlet of the first liquid tank, closing a first liquid outlet of the first liquid tank, such that the sample solution to be detected enters the first liquid tank, and pressure in the first liquid tank is gradually increased, and under the pressure, the sample solution to be detected flows upward from the first ends of the first channels of the plurality of microfluidic channels, enters the plurality of first channels, and is squeezed by the first channels to be separated into a plurality of sample droplets, and the plurality of sample droplets ente

Assignees

• Boe Technology Group Co Ltd

Inventors

• Ding Ding
• Deng Lin

Classifications

• B01L3/502792 Primary

  for moving individual droplets on a plate, e.g. by locally altering surface tension · CPC title

• B01L2400/0478

  pistons · CPC title

• B01L3/5027 Primary

  by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip · CPC title

• B01L3/502784 Primary

  specially adapted for droplet or plug flow, e.g. digital microfluidics · CPC title

• B01L3/502769

  characterised by multiphase flow arrangements · CPC title