Separating composite liquids

Patent metadata

Field	Value
Publication number	US-11692170-B2
Application number	US-202016915818-A
Country	US
Kind code	B2
Filing date	Jun 29, 2020
Priority date	Feb 18, 2013
Publication date	Jul 4, 2023
Grant date	Jul 4, 2023

Abstract

Described are embodiments that include methods and devices for separating composite liquids into components. Embodiments involve the use of a flexible membrane for separating a composite liquid into components. The composite liquid may include, in embodiments, a cellular containing liquid, such as whole blood or components of whole blood. In one specific embodiment, the composite liquid is a buffy coat.

First claim

What is claimed is:

1. A method of separating components of whole blood, the method comprising:
transferring a liquid comprising whole blood into a blood processing vessel adapted to be mounted on a rotor;
separating the liquid comprising whole blood into at least a first component comprising cells and a second component by rotating the blood processing vessel on the rotor;
transferring the first component into a membrane volume defined by a flexible membrane movable from a contracted configuration to an expanded configuration, an inlet into the membrane volume is in fluid communication with an outlet of the blood processing vessel such that the first component flows from the blood processing vessel into the membrane volume for further separation, the flexible membrane is seated in a chamber volume defined by a stepped side wall of a cell separation chamber such that upon transferring the first component into the flexible membrane the flexible membrane expands from the contracted configuration to the expanded configuration, in the expanded configuration the flexible membrane contacts the stepped side wall to give the flexible membrane an expanded, stepped configuration;
separating cells from the first component in the membrane volume by rotating the cell separation chamber; and
removing the cells from the membrane volume, thereby allowing the flexible membrane to contract from the expanded, stepped configuration to the contracted configuration.

2. The method of claim 1 , wherein during the removing the cells from the membrane volume, the membrane volume is reduced by contracting of the flexible membrane.

3. The method of claim 1 wherein the membrane volume is offset from an axis of rotation of the rotor.

4. The method of claim 3 wherein the first component is transferred into the membrane volume at a first location adjacent the axis of rotation.

5. The method of claim 4 wherein the cells are removed from the membrane volume at a second location adjacent the axis of rotation.

6. The method of claim 4 wherein the cells are transferred into the membrane volume through a tube extending into the membrane volume.

7. The method of claim 3 wherein the membrane volume is located within the separation chamber, said separation chamber defining a predetermined chamber volume, and wherein said membrane volume expands and contracts within said chamber volume.

8. The method of claim 7 wherein the stepped side wall is a frustro-conical side wall.

9. The method of claim 3 wherein the liquid comprising whole blood can flow substantially continuously into the blood processing vessel.

10. The method of claim 1 , wherein the flexible membrane has a thickness that tapers along a length of the flexible membrane.

11. The method of claim 1 , wherein the first component includes a buffy coat having white blood cells;
wherein the separating cells from the first component in the membrane volume includes separating white blood cells from the buffy coat; and
wherein the removing the cells from the membrane volume includes removing the white blood cells from the membrane volume.

Assignees

• Terumo Bct Inc

Inventors

• Ladtkow James R
• Uhl Geoffrey M
• Kolenbrander Jeremy P
• Stanton Briden Ray

Classifications

• A61M1/3616

  Batch-type treatment · CPC title

• B04B2005/0471

  with additional elutriation separation of different particles · CPC title

• A61M1/3496

  Plasmapheresis; Leucopheresis; Lymphopheresis (A61M1/3472 takes precedence) · CPC title

• A61M1/262

  rotating · CPC title

• A61M1/3693

  using separation based on different densities of components, e.g. centrifuging · CPC title