Systems and methods for analyzing an analyte extracted from a sample using an adsorbent material

Patent metadata

Field	Value
Publication number	US-11680876-B2
Application number	US-202117346576-A
Country	US
Kind code	B2
Filing date	Jun 14, 2021
Priority date	Jun 3, 2016
Publication date	Jun 20, 2023
Grant date	Jun 20, 2023

Abstract

Systems and methods for analyzing an analyte extracted from a sample using an adsorbent material.

First claim

What is claimed is:

1. A method for extracting an analyte from a sample, the method comprising:
introducing a sample into a capillary that comprises an adsorbent material that coats a portion of walls of the capillary;
interacting the sample within the capillary with the adsorbent material within the capillary such that at least one analyte is extracted from the sample and binds to the adsorbent material;
introducing an extraction solvent into the capillary while the sample is still present in the capillary; and
interacting the extraction solvent with the adsorbent material in order to elute the at least one analyte from the adsorbent material, wherein the extraction occurs via iterative back and forth movement of the capillary to results in the adsorbent material interacting with the sample and the extraction solvent over multiple occurrences.

2. The method according to claim 1 , wherein the capillary is moved a first time to cause the sample to interact with the adsorbent material and then moved a second time to cause the extraction solvent to interact with the adsorbent material after the sample has interacted with the adsorbent material.

3. The method according to claim 2 , wherein the sample and the extraction solvent are both within the capillary and are separated from each other by an air gap.

4. The method according to claim 1 , further comprising analyzing the extracted at least one analyte.

5. The method according to claim 4 , wherein analyzing comprises:
applying a voltage to the extraction solvent comprising the at least one analyte in the capillary so that the at least one analyte is expelled from the capillary, thereby generating ions of the at least one analyte; and
analyzing the ions.

6. The method according to claim 4 , wherein analyzing comprises:
removing the solvent comprising the at least one analyte from the capillary; and
conducting an assay that analyzes the at least one analyte.

7. The method according to claim 1 , wherein the solvent is immiscible with the sample.

8. The method according to claim 1 , wherein the solvent is miscible with the sample.

9. The method according to claim 1 , wherein the adsorbent material comprises one or more molecules that react with the at least one analyte.

10. The method according to claim 9 , further comprising conducting a reaction between the one or more molecules and the at least one analyte to produce a reaction product.

11. The method according to claim 10 , further comprising analyzing the reaction product.

12. The method according to claim 11 , wherein analyzing comprises:
applying a voltage to the extraction solvent comprising the reaction product in the capillary so that the reaction product is expelled from the capillary, thereby generating ions of the reaction product; and
analyzing the ions.

13. The method according to claim 1 , wherein the extraction solvent comprises one or more molecules that react with the at least one analyte.

14. The method according to claim 13 , further comprising conducting a reaction between the one or more molecules and the at least one analyte to produce a reaction product.

15. The method according to claim 14 , further comprising analyzing the reaction product.

16. The method according to claim 15 , wherein analyzing comprises:
applying a voltage to the extraction solvent comprising the reaction product in the capillary so that the reaction product is expelled from the capillary, thereby generating ions of the reaction product; and
analyzing the ions.

17. The method according to claim 15 , wherein analyzing comprises:
removing the extraction solvent comprising the reaction product from the capillary; and
conducting an assay that analyzes the reaction product.

18. The method according to claim 1 , wherein the adsorbent material includes an internal standard.

Assignees

• Purdue Research Foundation

Inventors

• Ouyang Zheng
• Zhang Wenpeng

Classifications

• B01D15/10

  characterised by constructional or operational features · CPC title

• G01N2001/4061

  Solvent extraction · CPC title

• B01D11/00

  Solvent extraction · CPC title

• G01N1/405 Primary

  by adsorption or absorption · CPC title

• Y10T436/255

  including use of a solid sorbent, semipermeable membrane, or liquid extraction · CPC title