Systems and methods for quantifying an analyte extracted from a sample

Patent metadata

Field	Value
Publication number	US-2016201051-A1
Application number	US-201615064865-A
Country	US
Kind code	A1
Filing date	Mar 9, 2016
Priority date	Feb 21, 2014
Publication date	Jul 14, 2016
Grant date	—

Abstract

The invention generally relates to systems and methods for quantifying an analyte extracted from a sample. In certain embodiments, the invention provides methods that involve introducing a solvent into a capillary, introducing the capillary into a vessel including a sample such that a portion of the sample is introduced into the capillary, moving the sample and the solvent within the capillary to induce circulation within the sample and the solvent, thereby causing the analyte to be extracted from the sample and into the solvent, analyzing the analyte that has been extracted from the sample, and quantifying the analyte. In certain embodiments, the quantifying step is performed without knowledge of a volume of the sample and/or solvent.

First claim

What is claimed is:

1 . A method for extracting an analyte from a sample, the method comprising:
introducing a solvent into a capillary; introducing the capillary into a vessel comprising a sample such that a portion of the sample is introduced into the capillary; moving the sample and the solvent within the capillary to induce circulation within the sample and the solvent, thereby causing the analyte to be extracted from the sample and into the solvent; analyzing the analyte that has been extracted from the sample; and quantifying the analyte.

2 . The method according to claim 1 , wherein the quantifying step is performed without knowledge of a volume of the sample.

3 . The method according to claim 1 , wherein the quantifying step is performed without knowledge of a volume of the solvent.

4 . The method according to claim 1 , wherein analyzing comprises:
applying a voltage to the solvent comprising the extracted analyte in the capillary so that the analyte is expelled from the capillary, thereby generating ions of the analyte; and analyzing the ions.

5 . The method according to claim 1 , wherein analyzing comprises:
removing the solvent comprising the extracted analyte from the capillary; and conducting an assay that analyzes the analyte.

6 . The method according to claim 5 , wherein the assay comprises:
generating ions of the analyte; and analyzing the ions

7 . The method according to claim 1 , wherein the solvent comprises an internal standard.

8 . The method according to claim 1 , wherein the sample comprises an internal standard.

9 . The method according to claim 1 , wherein the solvent is immiscible with the sample.

10 . The method according to claim 1 , wherein the solvent is miscible with the sample and the method further comprises introducing a bridging solvent into the capillary that is immiscible with the solvent and the sample in a manner in which the bridging solvent is between the solvent and the sample.

11 . A method for extracting an analyte from a sample, the method comprising:
introducing a solvent into a capillary; introducing the capillary into a vessel comprising a sample such that a portion of the sample is introduced into the capillary; and moving the sample and the solvent within the capillary to induce circulation within the sample and the solvent, thereby causing the analyte to be extracted from the sample and into the solvent.

12 . The method according to claim 11 , further comprising analyzing the extracted analyte.

13 . The method according to claim 12 , wherein analyzing comprises:
applying a voltage to the solvent comprising the extracted analyte in the capillary so that the analyte is expelled from the capillary, thereby generating ions of the analyte; and analyzing the ions.

14 . The method according to claim 12 , wherein analyzing comprises:
removing the solvent comprising the extracted analyte from the capillary; and conducting an assay that analyzes the analyte.

15 . The method according to claim 14 , wherein the assay comprises:
generating ions of the analyte; and analyzing the ions

16 . The method according to claim 11 , wherein the solvent is introduced to the capillary first.

17 . The method according to claim 11 , wherein the sample is introduced to the capillary first.

18 . The method according to claim 11 , wherein the solvent comprises an internal standard.

19 . The method according to claim 11 , wherein the solvent is immiscible with the sample.

20 . The method according to claim 11 , wherein the solvent is miscible with the sample and the method further comprises introducing a bridging solvent into the capillary that is immiscible with the solvent and the sample in a manner in which the bridging solvent is between the solvent and the sample.

Assignees

• Purdue Research Foundation

Inventors

• Ouyang Zheng
• Ren Yue
• Lin Ziqing

Classifications

• C12N15/1003 Primary

  Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor · CPC title

• H01J49/0404

  Capillaries used for transferring samples or ions (electrospray nozzles H01J49/167) · CPC title

• C12Q1/6806

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title

• H01J49/0431

  for liquid samples (interfaces to liquid chromatographs G01N30/7233) · CPC title

• H01J49/167 Primary

  Capillaries and nozzles specially adapted therefor; (electrostatic spraying per se B05B5/00) · CPC title