Solution mixer, fluidic device, and solution mixing method

Patent metadata

Field	Value
Publication number	US-11642636-B2
Application number	US-201816216299-A
Country	US
Kind code	B2
Filing date	Dec 11, 2018
Priority date	Sep 25, 2013
Publication date	May 9, 2023
Grant date	May 9, 2023

Abstract

A solution mixer comprising: a main flow path in which a solution circulates; at least one solution introduction flow path connected to the main flow path; and at least one solution discharge flow path connected to the main flow path, wherein the solution discharge flow path has at least one solution discharge flow path valve, and wherein the main flow path has at least one main flow path valve.

First claim

What is claimed is:

1. A method of mixing a plurality of solutions in a solution mixer comprising:
a looped main flow path;
two main flow path valves that are disposed on the looped main flow path and compartmentalize the looped main flow path into a first partial region and a second partial region such that a volume ratio of the first partial region to the second partial region is a predetermined volume ratio;
a first introduction flow path directly connected to the first partial region;
a second introduction flow path directly connected to the second partial region;
a first introduction flow path valve disposed in the first introduction flow path;
a second introduction flow path valve disposed in the second introduction flow path;
a first discharge flow path connected to the first partial region;
a first discharge flow path valve disposed in the first discharge flow path;
a second discharge flow path connected to the second partial region; and
a second discharge flow path valve disposed in the second discharge flow path;
the method comprising:
(A) introducing a first solution to fill the first partial region, and a second, different solution which is different from the first solution to fill the first partial region and the second partial region, respectively; and
(B) mixing the first solution and the second solution by the first partial region and the second partial region communicating with each other via the main flow path valves.

2. The method according to claim 1 ,
wherein at least one of the main flow path valves is disposed in the vicinity of one of the first and second solution introduction flow paths and/or in the vicinity of the solution discharge flow path.

3. The method according to claim 1 ,
wherein the looped main flow path includes a first flow path, a second flow path, and first and second connecting flow paths which respectively allow communication with the first flow path and the second flow path, and
wherein at least one of the main flow path valves is disposed in the first connecting flow path and/or the second connecting flow path.

4. The method according to claim 1 , further comprising a pump which circulates at least one of the first and second solutions in the looped main flow path.

5. The method according to claim 4 ,
wherein the pump includes at least three pump valves.

6. The method according to claim 1 ,
wherein the looped main flow path includes a detection unit that detects a substance in one or both of the first and second solutions.

7. The method according to claim 6 ,
wherein the substance in the solution is a biomolecule, and
wherein the detection unit includes a substrate to which a substance having affinity to the biomolecule is immobilized.

8. A method of mixing two types of solutions with each other in a solution mixer comprising:
a looped main flow path;
two main flow path valves that are disposed on the looped main flow path and compartmentalize the looped main flow path into a first partial region and a second partial region such that a volume ratio of the first partial region to the second partial region is a predetermined volume ratio;
a first introduction flow path directly connected to the first partial region;
a second introduction flow path directly connected to the second partial region;
a first introduction flow path valve disposed in the first introduction flow path;
a second introduction flow path valve disposed in the second introduction flow path;
a first discharge flow path connected to the first partial region;
a first discharge flow path valve disposed in the first discharge flow path;
a second discharge flow path connected to the second partial region; and
a second discharge flow path valve disposed in the second discharge flow path;
the method comprising:
(A) sending a first solution to the first partial region via the looped main flow path from the first solution introduction flow path, while the two main flow path valves and the solution discharge flow path valve are open, to fill the first partial region;
(B) closing the two main flow path valves;
(C) sending a second, different solution to the second partial region via the looped main flow path from the second solution introduction flow path, to fill the second partial region;
(D) closing the solution discharge flow path valve; and
(E) circulating and mixing the first solution and the second solution in the looped main flow path by opening the two main flow path valves.

9. A method of mixing a plurality of solutions with each other in a solution mixer comprising:
a first flow path, a second flow path, and first and second connecting flow paths which respectively allow communication with the first flow path and the second flow path,
first and second solution introduction flow paths which are respectively connected to the first and second flow paths,
first and second solution discharge flow paths which are respectively connected to the first and second flow paths,
first and second solution discharge flow path valves which are respectively disposed in the first and second solution discharge flow paths, and
first and second main flow path valves which are respectively disposed in the first and second connecting flow paths, the first and second main flow path valves being disposed such that each of partial regions of the main flow path, which is compartmentalized by closing the first and second main flow path valves, has a predetermined volume, and
wherein the method comprises:
(A) introducing a first solution into the first flow path from the first solution introduction flow path and introducing a second, different solution into the second flow path from the second solution introduction flow path to fill one or more of the partial regions of the main flow path, while the first and second main flow path valves are closed and the first and second solution discharge flow path valves are open; and
(B) circulating and mixing the first and second solutions by closing the first and second solution discharge flow path valves and opening the first and second main flow path valves.

10. The method according to claim 9 ,
wherein the solution mixer further includes
a third flow path, and third and fourth connecting flow paths which respectively allow communication with the second and third flow paths,
a third solution introduction flow path which is connected to the third flow path,
a third solution discharge flow path which is connected to the third flow path,
a third solution discharge flow path valve which is disposed in the third solution discharge flow path, and
third and fourth main flow path valves which are respectively disposed in the third and fourth connecting flow paths, and
wherein the method further comprises:
(C) introducing a third solution into the third flow path from the third solution introduction flow path to fill another of the partial regions of the main flow path, while the third solution discharge flow path valve is open, before or after (B); and
(D) circulating and mixing the third solution with a mixed solution of the first and second solutions by closing the third solution discharge flow path valve and opening the third and fourth main flow path valves, after (B) and (C).

11. A method of mixing a plurality of solutions with each other in a solution mixer comprising:
a looped main flow path, in which a solution circulates, the looped main flow path including a first flow path, a second flow path, a third flow path, first and second connecting flow paths which allow communication with the first flow path and the second flow path, and third and fourth connecting flow paths which allow communicatio

Assignees

• Univ Tokyo
• Nikon Corp

Inventors

• Ichiki Takanori
• Ueno Taro
• Tsuchiya Shoichi
• Kobayashi Masashi
• Miyamoto Kenji

Classifications

• B01F33/30

  Micromixers · CPC title

• B01F25/51 Primary

  in which the mixture is circulated through a set of tubes, e.g. with gradual introduction of a component into the circulating flow · CPC title

• G01N2035/00158

  Elements containing microarrays, i.e. "biochip" · CPC title

• B01F23/451

  by injecting one liquid into another · CPC title

• B01F25/54 Primary

  provided with a pump inside the receptacle to recirculate the material within the receptacle · CPC title