Promoter, promoter control elements, and combinations, and uses thereof

Patent metadata

Field	Value
Publication number	US-11634723-B2
Application number	US-202016938557-A
Country	US
Kind code	B2
Filing date	Jul 24, 2020
Priority date	Sep 11, 2003
Publication date	Apr 25, 2023
Grant date	Apr 25, 2023

Abstract

The present invention is directed to promoter sequences and promoter control elements, polynucleotide constructs comprising the promoters and control elements, and methods of identifying the promoters, control elements, or fragments thereof. The invention further relates to the use of the present promoters or promoter control elements to modulate transcript levels.

First claim

What is claimed is:

1. A vector construct comprising:
a) a first nucleic acid comprising
(i) the nucleic acid sequence of SEQ ID NO: 148 wherein the nucleic acid sequence of SEQ ID NO: 148 has a promotor activity,
(ii) a nucleic acid sequence having at least 96 percent sequence identity to SEQ ID NO: 148 and having the promoter activity of SEQ ID NO:148, or
(iii) a fragment of at least 500 consecutive nucleotides of the nucleic acid sequence of SEQ ID NO:148 having the promoter activity of SEQ ID NO:148; and

b) a second nucleic acid that is transcribable;
wherein said first and second nucleic acid molecules are heterologous to each other and are operably linked together.

2. The vector construct according to claim 1 , wherein said first nucleic acid comprises a nucleic acid sequence having at least 97 percent sequence identity to SEQ ID NO:148.

3. The vector construct according to claim 1 , wherein said first nucleic acid comprises the nucleic acid sequence of SEQ ID NO: 148.

4. A host cell comprising the vector construct according to claim 1 .

5. The host cell according to claim 4 , wherein said first nucleic acid comprises a nucleic acid sequence having at least 97 percent sequence identity to SEQ ID NO: 148.

6. The host cell according to claim 4 , wherein said first nucleic acid comprises the nucleic acid sequence of SEQ ID NO:148.

7. A plant cell comprising the vector construct of claim 1 .

8. A method of modulating transcription by combining, in an environment suitable for transcription:
a) a first nucleic acid comprising
(i) the nucleic acid sequence of SEQ ID NO:148 wherein the nucleic acid sequence of SEQ ID NO:148 has a promotor activity,
(ii) a nucleic acid sequence having at least 96 percent sequence identity to SEQ ID NO:148 and having the promoter activity of SEQ ID NO:148, or
(iii) a fragment of at least 500 consecutive nucleotides of the nucleic acid sequence of SEQ ID NO:148 having the promoter activity of SEQ ID NO:148; and

b) a second nucleic acid to be transcribed; wherein the first and second nucleic acid molecules are heterologous to each other and operably linked together.

9. The method of claim 8 , wherein said first nucleic acid comprises a nucleic acid sequence having at least 97 percent sequence identity to SEQ ID NO:148.

10. The method of claim 8 , wherein said first nucleic acid comprises a nucleic acid sequence having at least 98 percent sequence identity to SEQ ID NO:148.

11. The method of claim 8 , wherein said first nucleic acid comprises the nucleic acid sequence of SEQ ID NO:148.

12. A plant comprising the vector construct according to claim 1 .

Assignees

• Ceres Inc

Inventors

• Cook Zhihong
• Fang Yiwen
• Feldmann Kenneth A
• Kiegle Edward
• Kwok Shing
• Lu Yu-Ping
• Medrano Leonard
• Pennell Roger
• Schneeberger Richard
• Wu Chuan-Yin
• Apuya Nestor
• Okamuro Jack K
• Jofuku Diane K
• Donson Jonathan
• Dang David Van-Dinh
• Margolles-Clark Emilio
• Alexandrov Nickolai
• Tatarinova Tatiana
• Theiss Noah
• Grizard Danielle
• Davis Shawna
• Robles Dennis
• Portereiko Michael

Classifications

• C12N15/8226

  Stem-specific, e.g. including tubers, beets · CPC title

• C12N15/823

  Reproductive tissue-specific promoters · CPC title

• C12N15/8222 Primary

  Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation · CPC title

• C12N15/8227

  Root-specific · CPC title

• C12N15/8225

  Leaf-specific, e.g. including petioles, stomata · CPC title