Promoter, promoter control elements, and combinations, and uses thereof

Patent metadata

Field	Value
Publication number	US-10851383-B2
Application number	US-201815967437-A
Country	US
Kind code	B2
Filing date	Apr 30, 2018
Priority date	Oct 14, 2003
Publication date	Dec 1, 2020
Grant date	Dec 1, 2020

Abstract

The present invention is directed to promoter sequences and promoter control elements, polynucleotide constructs comprising the promoters and control elements, and methods of identifying the promoters, control elements, or fragments thereof. The invention further relates to the use of the present promoters or promoter control elements to modulate transcript levels.

First claim

What is claimed is:

1. A vector construct comprising:
a) a first nucleic acid comprising (i) the nucleic acid sequence of SEQ ID NO:220 or 221, or (ii) a nucleic acid sequence having at least 95% sequence identity to SEQ ID NO:220 or 221 and having promoter activity; and
b) a second nucleic acid that is transcribable;
wherein said first and second nucleic acids are heterologous to each other and are operably linked together.

2. The vector construct according to claim 1 , wherein said first nucleic acid comprises a nucleic acid sequence having at least 98% sequence identity to SEQ ID NO:220 or 221.

3. The vector construct according to claim 1 , wherein said first nucleic acid comprises the nucleic acid sequence of SEQ ID NO:220 or 221.

4. A host cell comprising the vector construct according to claim 1 .

5. The host cell according to claim 4 , wherein said first nucleic acid comprises a nucleic acid sequence having at least 98% sequence identity to SEQ ID NO:220 or 221.

6. The host cell according to claim 4 , wherein said first nucleic acid comprises the nucleic acid sequence of SEQ ID NO:220 or 221.

7. A plant cell comprising the vector construct of claim 1 .

8. A method of modulating transcription by combining, in an environment suitable for transcription:
a) a first nucleic acid comprising (i) the nucleic acid sequence of SEQ ID NO:220 or 221, or (ii) a nucleic acid sequence having at least 95% sequence identity to SEQ ID NO:220 or 221 having promoter activity; and
b) a second nucleic acid to be transcribed;
wherein the first and second nucleic acids are heterologous to each other and operably linked together.

9. The method of claim 8 , wherein said first nucleic acid comprises a nucleic acid sequence having at least 97% sequence identity to SEQ ID NO:220 or 221.

10. The method of claim 8 , wherein said first nucleic acid comprises a nucleic acid sequence having at least 98% sequence identity to SEQ ID NO:220 or 221.

11. The method according to any one of claim 8 , wherein said first nucleic acid comprises the nucleic acid sequence of SEQ ID NO:220 or 221.

12. A plant comprising the vector construct according to claim 1 .

Assignees

• Ceres Inc

Inventors

• Cook Zhihong
• Fang Yiwen
• Feldmann Kenneth A
• Kiegle Edward
• Kwok Shing
• Lu Yu-Ping
• Medrano Leonard
• Pennell Roger
• Schneeberger Richard
• Wu Chuan-Yin
• Apuya Nestor
• Okamuro Jack K
• Jofuku Diane K
• Donson Jonathan
• Dang David Van-Dinh
• Margolles-Clark Emilio
• Alexandrov Nickolai
• Tatarinova Tatiana
• Theiss Noah
• Grizard Danielle
• Davis Shawna
• Robles Dennis
• Portereiko Michael

Classifications

• C07K14/415 Primary

  from plants · CPC title

• C12N15/8234

  Seed-specific, e.g. embryo, endosperm · CPC title

• C12N15/8226

  Stem-specific, e.g. including tubers, beets · CPC title

• C12N15/8237

  Externally regulated expression systems · CPC title

• C12N15/8225

  Leaf-specific, e.g. including petioles, stomata · CPC title