Lipid comprising docosapentaenoic acid

Patent metadata

Field	Value
Publication number	US-11623911-B2
Application number	US-201816146469-A
Country	US
Kind code	B2
Filing date	Sep 28, 2018
Priority date	Dec 18, 2013
Publication date	Apr 11, 2023
Grant date	Apr 11, 2023

Abstract

The present invention relates to extracted plant lipid or microbial lipid comprising docosapentaenoic acid, and processes for producing the extracted lipid.

First claim

The invention claimed is:

1. Brassica napus seedoil, comprising fatty acids in an esterified form, the fatty acids comprising oleic acid, palmitic acid, ω6 polyunsaturated fatty acids which comprise linoleic acid (LA), ω3 polyunsaturated fatty acids which comprise α-linolenic acid (ALA), eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), docosahexaenoic acid (DHA), stearidonic acid (SDA) and eicosatetraenoic acid (ETA), wherein the level of palmitic acid in the total fatty acid content of the seedoil is between 2% and 16% by weight, wherein myristic acid (C14:0) is either absent or is present in the seedoil at a level of less than 1% by weight of the total fatty acid content of the seedoil, and wherein the level of EPA in the total fatty acid content of the seedoil is between 4% and 15% by weight.

2. The seedoil of claim 1 , wherein a level of γ-linolenic acid (GLA) of less than 4% by weight is present in the total fatty acid content of the seedoil.

3. The seedoil of claim 2 , wherein at least 70% of the DPA esterified in the form of triacylglycerols (TAG) in the seedoil is in the sn-1 or sn-3 position of the TAG.

4. The seedoil of claim 2 , wherein at least 80% of the DPA esterified in the form of TAG in the seedoil is in the sn-1 or sn-3 position of the TAG.

5. The seedoil of claim 2 , wherein the level of docosahexaenoic acid (DHA) in the total fatty acid content of the seedoil is less than 0.5% by weight.

6. The seedoil of claim 2 , wherein the level of docosahexaenoic acid (DHA) in the total fatty acid content of the seedoil is less than 2% by weight.

7. The seedoil of claim 2 , wherein the level of docosahexaenoic acid (DHA) in the total fatty acid content of the seedoil is between 0.5% and 2.0% by weight.

8. The seedoil of claim 7 , wherein at least 70% of the DHA esterified in the form of triacylglycerols (TAG) in the seedoil is in the sn-1 or sn-3 position of the TAG.

9. The seedoil of claim 2 , wherein the level of docosapentaenoic acid (DPA) in the total fatty acid content of the seedoil is between 0.05% and 8% by weight.

10. The seedoil of claim 9 , wherein at least 70% of the DPA esterified in the form of triacylglycerols (TAG) in the seedoil is in the sn-1 or sn-3 position of the TAG.

11. The seedoil of claim 3 , which has the following features
i) the level of oleic acid in the total fatty acid content of the seedoil is between 1% and 60% by weight,
ii) the level of linoleic acid (LA) in the total fatty acid content of the seedoil is between 4% and 35% by weight,
iii) a level of γ-linolenic acid (GLA) of less than 4% by weight is present in the total fatty acid content of the seedoil, and
iv) a level of total saturated fatty acids of between 4% and 25% by weight is present in the total fatty acid content of the seedoil.

12. The seedoil of claim 10 , wherein the level of docosahexaenoic acid (DHA) in the total fatty acid content of the seedoil is between 0.5% and 2.0% by weight.

13. Brassica napus seedoil, comprising fatty acids in an esterified form, the fatty acids comprising oleic acid, palmitic acid, ω6 polyunsaturated fatty acids which comprise linoleic acid (LA), ω3 polyunsaturated fatty acids which comprise α-linolenic acid (ALA), eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), docosahexaenoic acid (DHA), stearidonic acid (SDA) and eicosatetraenoic acid (ETA), wherein the level of palmitic acid in the total fatty acid content of the seedoil is between 2% and 16% by weight, wherein the level of myristic acid (C14:0) is either absent or is present in the seedoil at a level of less than 1% by weight of the total fatty acid content of the seedoil, and wherein the level of DPA in the total fatty acid content of the seedoil is greater than the level of DHA.

14. The seedoil of claim 13 , wherein the level of DPA in the total fatty acid content of the seedoil is about 2.5% by weight.

15. The seedoil of claim 13 , wherein the level of DPA in the total fatty acid content of the seedoil is about 3.9% by weight and the level of DHA is about 1.2% by weight.

16. The seedoil of claim 13 , wherein the level of DPA in the total fatty acid content of the seedoil is about 3.0% by weight and the level of DHA is about 0.8% by weight.

17. The seedoil of claim 13 , wherein a level of γ-linolenic acid (GLA) of less than 4% by weight is present in the total fatty acid content of the seedoil.

18. The seedoil of claim 17 , wherein at least 70% of the DPA esterified in the form of triacylglycerols (TAG) in the seedoil is in the sn-1 or sn-3 position of the TAG.

19. The seedoil of claim 17 , wherein at least 80% of the DPA esterified in the form of TAG in the seedoil is in the sn-1 or sn-3 position of the TAG.

20. The seedoil of claim 17 , wherein the level of docosahexaenoic acid (DHA) in the total fatty acid content of the seedoil is less than 0.5% by weight.

21. The seedoil of claim 17 , wherein the level of docosahexaenoic acid (DHA) in the total fatty acid content of the seedoil is less than 2% by weight.

22. The seedoil of claim 17 , wherein the level of docosahexaenoic acid (DHA) in the total fatty acid content of the seedoil is between 0.5% and 2.0% by weight.

23. The seedoil of claim 22 , wherein at least 70% of the DHA esterified in the form of triacylglycerols (TAG) in the seedoil is in the sn-1 or sn-3 position of the TAG.

24. The seedoil of claim 17 , wherein the level of docosapentaenoic acid (DPA) in the total fatty acid content of the seedoil is between 0.05% and 8% by weight.

25. The seedoil of claim 24 , wherein at least 70% of the DPA esterified in the form of triacylglycerols (TAG) in the seedoil is in the sn-1 or sn-3 position of the TAG.

26. The seedoil of claim 18 , which has the following features
i) the level of oleic acid in the total fatty acid content of the seedoil is between 1% and 60% by weight,
ii) the level of linoleic acid (LA) in the total fatty acid content of the seedoil is between 4% and 35% by weight,
iii) a level of γ-linolenic acid (GLA) of less than 4% by weight is present in the total fatty acid content of the seedoil, and
iv) a level of total saturated fatty acids of between 4% and 25% by weight is present in the total fatty acid content of the seedoil.

27. The seedoil of claim 25 , wherein the level of docosahexaenoic acid (DHA) in the total fatty acid content of the seedoil is between 0.5% and 2.0% by weight.

28. Brassica napus seedoil, comprising a total fatty acid content which comprises fatty acids in an esterified polyunsaturated fatty acids which comprise linoleic acid (LA), ω3 polyunsaturated fatty acids which comprise α-linolenic acid (ALA), eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), docosahexaenoic acid (DHA), stearidonic acid (SDA) and eicosatetraenoic acid (ETA), wherein the level of palmitic acid in the total fatty acid content of the seedoil is between 2% and 16% by weight, wherein myristic acid (C14:0) is either absent or is present in the seedoil at a level of less than 1% by weight of the total fatty acid content of the seedoil, wherein at least 70% of the DPA esterified in the form of triacylglycerols (TAG) in the seedoil is in the sn-1 or sn-3 position of the TAG, and wherein ALA, SDA, ETA, EPA, DPA, DHA and eicosatrienoic acid (ETrA) are each present at a level in the total fatty acid content, each level being expressed as a percentage of the total fatty acid content, wh

Assignees

• Commw Scient Ind Res Org
• Grains Res & Dev Corp
• Nuseed Nutritional Australia Pty Ltd

Inventors

• Petrie James Robertson
• Singh Surinder Pal
• Shrestha Pushkar
• Mcallister Jason Timothy
• De Feyter Robert Charles
• Devine Malcolm David

Classifications

• A61K36/31

  Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi · CPC title

• A23K10/30

  from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms (obtained by microbiological or biochemical processes, e.g. using yeasts or enzymes, A23K10/10) · CPC title

• A01H5/00

  Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy · CPC title

• C11C3/02 Primary

  by esterification of fatty acids with glycerol · CPC title

• A01H5/10

  Seeds · CPC title