Polypeptides

Patent metadata

Field	Value
Publication number	US-11613741-B2
Application number	US-202117157137-A
Country	US
Kind code	B2
Filing date	Jan 25, 2021
Priority date	Oct 7, 2015
Publication date	Mar 28, 2023
Grant date	Mar 28, 2023

Abstract

The present invention relates to polypeptides, nucleotides encoding the polypeptide, as well as methods of producing the polypeptides. The present invention also relates to detergent composition comprising polypeptides, a laundering method and the use of polypeptides.

First claim

The invention claimed is:

1. A composition comprising:
(a) at least 0.002 ppm of a polypeptide having DNase activity, wherein the polypeptide comprises the motif HXXP (SEQ ID NO: 210), where H is histidine, P is proline and X is any amino acid and comprises one or both of the motifs [D/M/L][S/T]GYSR[D/N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205), and wherein the polypeptide has at least 80% sequence identity to the amino acid sequence of SEQ ID NO: 21,
(b) one or more polyol(s), and
(c) a surfactant,

wherein the composition is formulated as a bar, a homogenous tablet, and a tablet having two or more layers, a pouch having one or more compartments, a regular or compact powder, a granule, a paste, a gel, or a liquid.

2. The composition of claim 1 , wherein the polypeptide having DNase activity comprises one or more of the motifs selected from the motifs [T/D/S][G/N]PQL (SEQ ID NO: 198), [G/T]Y[D/S][R/K/L] (SEQ ID NO: 199), [E/D/H]H[I/V/L/F/M]X[P/A/S] (SEQ ID NO: 200), [F/L/Y/I]A[N/R]D[L/I/P/V] (SEQ ID NO: 201) and C[D/N]T[A/R] (SEQ ID NO: 202).

3. The composition of claim 1 , wherein the composition is a detergent composition.

4. The composition of claim 1 , wherein the polyol is selected from glycerol, (mono, di, or tri) propylene glycol, ethylene glycol, polyethylene glycol, sugar alcohols, sorbitol, mannitol, erythritol, dulcitol, inositol, xylitol and adonitol.

5. The composition of claim 1 , wherein the polypeptide having DNase activity has at least 85% sequence identity to the amino acid sequence of SEQ ID NO: 21.

6. The composition of claim 1 , further comprising a second enzyme.

7. The composition of claim 6 , wherein the second enzyme is a protease, amylase, or lipase.

8. The composition of claim 1 , wherein the polypeptide having DNase activity has at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 21.

9. The composition of claim 1 , wherein the polypeptide having DNase activity is a variant of the polypeptide of SEQ ID NO: 21, wherein the variant has DNase activity, wherein the variant has 1-25 amino acid alterations.

10. The composition of claim 9 , wherein the alterations are substitutions.

11. The composition of claim 1 , wherein the polypeptide having DNase activity is a variant of the polypeptide of SEQ ID NO: 21, wherein the variant has DNase activity, wherein the variant has 1-20 amino acid substitutions, deletions, and/or insertions.

12. The composition of claim 11 , wherein the alterations are substitutions.

Assignees

• Novozymes As

Inventors

• Gjermansen Morten
• Gori Klaus
• Geertz-Hansen Henrik Marcus
• Salomon Jesper
• Blicher Thomas Holberg
• Spodsberg Nikolaj

Classifications

• C11D3/3707

  Polyethers, e.g. polyalkyleneoxides · CPC title

• C11D3/38636

  containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase · CPC title

• C11D3/22

  Carbohydrates or derivatives thereof · CPC title

• C12Y301/21001

  Deoxyribonuclease I (3.1.21.1) · CPC title

• C11D3/38681

  Chemically modified or immobilised enzymes · CPC title