Method for detecting aldehyde and ketone by using thin layer chromatography

Patent metadata

Field	Value
Publication number	US-11604176-B2
Application number	US-201916761094-A
Country	US
Kind code	B2
Filing date	Jan 22, 2019
Priority date	Jun 11, 2018
Publication date	Mar 14, 2023
Grant date	Mar 14, 2023

Abstract

The present invention relates to a method for simultaneously qualitatively and quantitatively analyzing compounds of aldehydes and/or ketones in a short time by using an optimal TLC plate, a proportion of a developing solvent, a sample amount, etc., and can provide an analysis result equivalent to a conventional analysis result in a shorter time by providing the most optimal conditions when using the TLC method.

First claim

What is claimed is:

1. A method for analyzing aldehyde or ketone, comprising:
(i) injecting a sample of the aldehyde or the ketone into a cartridge containing 2,4-dinitrophenylhydrazine (2,4-DNPH) to obtain a 2,4-dinitrophenylhydrazone derivative;
(ii) extracting the 2,4-dinitrophenylhydrazone derivative obtained during the injecting with a solvent to produce an extract; and
(iii) analyzing the extract by thin layer chromatography (TLC) to identify aldehyde or ketone derivatives corresponding to the aldehyde or the ketone of the sample,
wherein the sample of the aldehyde or the ketone is injected in an amount of 0.3 to 0.7 μL, and
wherein the analyzing the extract by TLC is performed using a developing solvent which is a mixed solvent of ethyl acetate (EA) and hexane (Hex) of 1:8 to 1:12.

2. The method for analyzing aldehyde or ketone according to claim 1 , wherein the sample of aldehyde or ketone comprises at least one selected from of formaldehyde, acetaldehyde, acrolein, acetone, propionaldehyde, butyraldehyde, benzaldehyde, crotonaldehyde, iso-valeraldehyde, n-valeraldehyde, o-tolualdehyde, m-tolualdehyde, p-tolualdehyde, hexaldehyde and 2,5-dimethylbenzaldehyde.

3. The method for analyzing aldehyde or ketone according to claim 1 , wherein the solvent is acetonitrile (AN).

4. The method for analyzing aldehyde or ketone according to claim 1 , wherein the 2,4-dinitrophenylhydrazone derivative is at least one selected from the group consisting of formaldehyde-2,4-dinitrophenylhydrazone, acetaldehyde-2,4-dinitrophenylhydrazone, acrolein-2,4-dinitrophenylhydrazone, acetone-2,4-dinitrophenylhydrazone, propionaldehyde-2,4-dinitrophenylhydrazone, butyraldehyde-2,4-dinitrophenylhydrazone, benzaldehyde-2,4-dinitrophenylhydrazone, crotonaldehyde-2,4-dinitrophenylhydrazone, methacrolein-2,4-dinitrophenylhydrazone, 2-butanone-2,4-dinitrophenylhydrazone, valeraldehyde-2,4-dinitrophenylhydrazone, m-tolualdehyde-2,4-dinitrophenylhydrazone and hexaldehyde-2,4-dinitrophenylhydrazone.

Assignees

• Lg Chemical Ltd

Inventors

• Han Su Youn
• Kim Byoung Hyoun
• Park Byung Hyun
• Lee Gyeongjin

Classifications

• G01N30/94 Primary

  Development · CPC title

• G01N30/90

  Plate chromatography, e.g. thin layer or paper chromatography · CPC title

• G01N2030/884

  organic compounds · CPC title

• G01N30/91 Primary

  Application of the sample · CPC title