Bacillus isolates and uses thereof

Patent metadata

Field	Value
Publication number	US-11560342-B2
Application number	US-201816959430-A
Country	US
Kind code	B2
Filing date	Dec 27, 2018
Priority date	Jan 3, 2018
Publication date	Jan 24, 2023
Grant date	Jan 24, 2023

Abstract

Microbial compositions for application to plants, plant parts and plant seeds are provided for improvement of plant yield and/or other beneficial plant traits. Methods of making and applying microbial compositions or formulations to plants, plant parts or plant seeds or to growth media are further provided to increase or improve plant yield and/or other beneficial plant traits.

First claim

The invention claimed is:

1. An isolated Bacillus megaterium strain having the deposit accession number NRRL B-67533 or NRRL B-67534.

2. The isolated Bacillus megaterium strain of claim 1 , wherein the Bacillus megaterium strain having the deposit accession number NRRL B-67533 or NRRL B-67534 increases organic phosphate and inorganic phosphate availability and/or solubilization.

3. A biologically pure culture of Bacillus megaterium NRRL B-67533 or Bacillus megaterium NRRL B-67534.

4. The biologically pure culture of claim 3 , wherein said Bacillus megaterium NRRL B-67533 or Bacillus megaterium NRRL B-67534 increases organic phosphate and inorganic phosphate availability and/or solubilization.

5. An inoculant composition comprising Bacillus megaterium NRRL B-67533 and/or Bacillus megaterium NRRL B-67534 in an agriculturally acceptable carrier.

6. The inoculant composition of claim 5 , wherein said agriculturally acceptable carrier comprises:
one or more monosaccharides;
one or more disaccharides;
one or more maltodextrins;
one or more sugar alcohols;
one or more humic acids;
one or more fulvic acids;
one or more oxidation control components; or
one or more UV protectants.

7. The inoculant composition of claim 5 , further comprising one or more pesticides.

8. The inoculant composition of claim 5 , further comprising:
a) one more lipo-chitooligosaccharides; or
b) one or more chitooligosaccharides.

9. The inoculant composition of claim 5 , further comprising one or more chitinous compounds.

10. The inoculant composition of claim 5 , further comprising one or more flavonoids.

11. The inoculant composition of claim 5 , further comprising one or more diazotrophs.

12. The inoculant composition of claim 5 , further comprising one or more additional phosphate-solubilizing microorganisms.

13. The inoculant composition of claim 5 , further comprising one or more biopesticides.

14. The inoculant composition of any claim 5 , comprising about 1× 10 1 to about 1× 10 12 colony-forming units of Bacillus megaterium NRRL B-67533 or Bacillus megaterium NRRL B-67534 per gram or millileter of said inoculant composition.

15. A coated plant propagation material comprising a plant propagation material, wherein said coated plant propagation material comprises Bacillus megaterium NRRL B-67533 and/or Bacillus megaterium NRRL B-67534 in an amount ranging from about 1× 10 1 to about 1× 10 15 colony-forming units.

16. A kit comprising the coated plant propagation material of claim 15 and a container housing said coated plant propagation material.

17. A method of treating a plant seed comprising applying the isolated strain of claim 1 to an outer surface of said seed.

18. A method of enhancing crop yield comprising applying the isolated strain of claim 1 , the biologically pure culture of claim 2 or the inoculant composition of claim 3 to plant seed in an effective amount/concentration for enhancing the growth and/or yield of plants that grow from said seed when said seed is planted in a plant growth medium, optionally a soil.

19. The inoculant composition of claim 5 , wherein said Bacillus megaterium NRRL B-67533 and/or Bacillus megaterium NRRL B-67534 increases organic phosphate and inorganic phosphate availability and/or solubilization.

20. The inoculant composition of claim 6 , wherein:
said monosaccharide is arabinose, fructose or glucose;
said disaccharide is maltose, sucrose or trehalose;
said maltodextrin is one or more maltodextrins each or collectively having a DEV value of about 15 to about 20;
said sugar alcohol is arabitol, mannitol, sorbitol or xylitol;
said humic acid is potassium humate or sodium humate;
said fulvic acid is potassium fulvate or sodium fulvate;
said hygroscopic polymer is one or more albumins, alginates, celluloses, gums, methyl celluloses, nylons, pectins, polyacrylic acids, polycarbonates, polyethylene glycols (PEG), polyethylenimines (PEI), polylactides, polymethylacrylates (PMA), polyurethanes, polyvinyl alcohols (PVA), polyvinylpyrrolidones (PVP), propylene glycols, sodium carboxymethyl celluloses or starches;
said oxidation control component is one or more antioxidants or one or more oxygen scavengers, or
said UV protectant is one or more lignosulfites.

21. The inoculant composition of claim 20 , wherein:
said gum is cellulose gum, guar gum, gum insect, gum combretum or xantham gum;
said antioxidant is ascorbic acid, ascorbyl palmitate, ascorbyl stearate, calcium ascorbate, one or more carotenoids, lipoic acid, one or more phenolic compounds, potassium ascorbate, sodium ascorbate, one or more thiols, one or more tocopherols, one or more tocotrienols, ubiquinone or uric acid; or
said oxygen scavenger is ascorbic acid or sodium hydrogen carbonate.

22. The inoculant composition of claim 21 , wherein:
said phenolic compound is one or more flavonoids, flavones or flavonols; or
said thiol is glutathione, lipoic acid or N-acetyl cysteine.

23. The inoculant composition of claim 7 , wherein:
said pesticide is an acaricide, insecticide, nematicide, one or more fungicides, one or more gastropodicides, one or more herbicides, one or more rodenticides, optionally brodifacoum, bromadiolone, bromethalin, cholecalciferol, chlorophacinone, difethialone, diphacinone, strychnine, warfarin and/or zinc phosphide or one or more virucides.

24. The inoculant composition of claim 23 , wherein:
said acaricide, insecticide or nematicide is one or more carbamates, diamides, macrocyclic lactones, neonicotinoids, organophosphates, phenylpyrazoles, pyrethrins, spinosyns, synthetic pyrethroids, tetronic acids or tetramic acids;
said fungicide is one or more aromatic hydrocarbons, benzimidazoles, benzthiadiazole, carboxamides, carboxylic acid amides, morpholines, phenylamides, phosphonates, quinone outside inhibitors, thiazolidines, thiophanates, thiophene carboxamides or triazoles;
said gastropodicide is one or more iron phosphates, metaldehydes, methiocarbs or salts;
said herbicide is one or more acetyl CoA carboxylase (ACCase) inhibitors, acetolactate synthase (ALS) inhibitors, acetohydroxy acid synthase (AHAS) inhibitors, photosystem II inhibitors, photosystem I inhibitors, protoporphyrinogen oxidase (PPO or Protox) inhibitors, carotenoid biosynthesis inhibitors, enolpyruvyl shikimate-3-phosphate (EPSP) synthase inhibitor, glutamine synthetase inhibitor, dihydropteroate synthetase inhibitor, mitosis inhibitors, 4-hydroxyphenyl-pyruvate-dioxygenase (4-HPPD) inhibitors, synthetic auxins, auxin herbicide salts, auxin transport inhibitors, or nucleic acid inhibitors; or
said rodenticide is brodifacoum, bromadiolone, bromethalin, cholecalciferol, chlorophacinone, difethialone, diphacinone, strychnine, warfarin or zinc phosphide.

25. The inoculant composition of claim 8 , wherein:
a) said lipo-chitooligosaccharide is one or more lipo-chitooligosaccharides represented by the structure:

Assignees

• Monsanto Technology Llc

Inventors

• Lutfiyya Linda L
• Mccann Ryan T
• Schaecher Scott R

Classifications

• A01N63/22

  Bacillus · CPC title

• C05F11/08 Primary

  Organic fertilisers containing added bacterial cultures, mycelia or the like · CPC title

• C05G5/40

  Fertilisers incorporated into a matrix · CPC title

• A01C1/06

  Coating or dressing seed · CPC title

• C05F11/02

  from peat, brown coal, and similar vegetable deposits · CPC title