Compositions and methods for altering flowering and plant architecture to improve yield potential

Patent metadata

Field	Value
Publication number	US-11555201-B2
Application number	US-201715787359-A
Country	US
Kind code	B2
Filing date	Oct 18, 2017
Priority date	Oct 19, 2016
Publication date	Jan 17, 2023
Grant date	Jan 17, 2023

Abstract

The present invention provides recombinant DNA constructs, vectors and molecules useful for attenuating and/or refining the expression of a florigenic FT gene or transgene using targeting sequences of small RNA molecules. Transgenic plants, plant cells and tissues, and plant parts comprising the recombinant constructs, vectors, and molecules are also provided. Transgenic plants comprising a florigenic FT transgene may produce more bolls, siliques, fruits, nuts, or pods per node on the transgenic plant via suppression, relative to a control or wild type plant. Methods are further provided for introducing the recombinant DNA constructs, vectors, and molecules into a plant, and planting transgenic plants in the field including at higher densities. Transgenic plants of the present invention may provide greater yield potential than wild type or control plants.

First claim

What is claimed is:

1. A recombinant DNA construct comprising a first expression cassette and a second expression cassette, wherein the first expression cassette comprises a polynucleotide sequence encoding a florigenic Flowering Locus T (FT) protein comprising an amino acid sequence that is at least 90% identical to SEQ ID NO: 2 operably linked to a first plant expressible promoter, wherein the first plant expressible promoter is a vegetative stage promoter, and the second expression cassette comprises a transcribable DNA sequence encoding a RNA molecule comprising a targeting sequence that is at least 90% complementary to at least 21 consecutive nucleotides of the polynucleotide sequence of the first expression cassette, and wherein the transcribable DNA sequence is operably linked to a second plant expressible promoter, wherein the second plant expressible promoter is a late vegetative stage promoter and/or reproductive stage promoter.

2. The recombinant DNA construct of claim 1 , wherein the transcribable DNA sequence comprises a sequence that is at least 90% complementary to SEQ ID NO: 65, 68, or 69.

3. The recombinant DNA construct of claim 1 , wherein the florigenic FT protein further comprises one or more of the following amino acids: a tyrosine or other uncharged polar or nonpolar residue at the amino acid position of the florigenic FT protein corresponding to amino acid position 85 of SEQ ID NO: 14; a leucine or other nonpolar residue at the amino acid position of the florigenic FT protein corresponding to amino acid position 128 of SEQ ID NO: 14; and a tryptophan or other large nonpolar residue at the amino acid position of the florigenic FT protein corresponding to amino acid position 138 of SEQ ID NO: 14.

4. The recombinant DNA construct of claim 1 , wherein the florigenic FT protein does not have one or more of the following amino acids: a histidine at the amino acid position corresponding to position 85 of SEQ ID NO: 14; a lysine or arginine at the amino acid position corresponding to position 128 of SEQ ID NO: 14; and a serine, aspartic acid, glutamic acid, lysine or arginine at the amino acid position corresponding to position 138 of SEQ ID NO: 14.

5. The recombinant DNA construct of claim 1 , wherein the polynucleotide sequence is at least 90% identical to SEQ ID NO: 1.

6. The recombinant DNA construct of claim 1 , wherein the first plant expressible promoter comprises a polynucleotide sequence that is at least 95% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 31, 32, and 48.

7. The recombinant DNA construct of claim 1 , wherein the second plant expressible promoter comprises a polynucleotide sequence that is at least 95% identical to SEQ ID NO: 49.

8. The recombinant DNA construct of claim 1 , wherein the first plant expressible promoter is an early vegetative stage promoter.

9. The recombinant DNA construct of claim 1 , wherein the second plant expressible promoter is a reproductive stage preferred promoter.

10. The recombinant DNA construct of claim 1 , wherein the first plant expressible promoter initiates detectable expression of the polynucleotide sequence encoding the florigenic FT protein at an earlier developmental stage than the second plant expressible promoter initiates detectable expression of the transcribable DNA sequence.

11. A transgenic plant comprising an insertion of the recombinant DNA construct of claim 1 into the genome of at least one cell of the transgenic plant.

12. The transgenic plant of claim 11 , wherein the transgenic plant is soybean.

13. The transgenic plant of claim 12 , wherein the transgenic soybean plant produces more pods per node than a control plant not having the recombinant DNA construct.

14. The transgenic plant of claim 12 , wherein the transgenic soybean plant produces more flowers per node than a control plant not having the recombinant DNA construct.

15. The transgenic plant of claim 12 , wherein the transgenic soybean plant produces more seeds or pods per node of the transgenic plant than a control plant not having the recombinant DNA construct.

16. The transgenic plant of claim 12 , wherein the transgenic soybean plant flowers earlier than a control plant not having the recombinant DNA construct.

17. The transgenic plant or part thereof of claim 11 , wherein the transgenic plant has more floral racemes per node than a control plant not having the recombinant DNA construct.

18. The recombinant DNA construct of claim 1 , wherein the targeting sequence of the RNA molecule is at least 95% complementary to at least 21 consecutive nucleotides of the polynucleotide sequence of the first expression cassette or an mRNA encoded by the polynucleotide sequence of the first expression cassette.

19. The recombinant DNA construct of claim 1 , wherein the targeting sequence of the RNA molecule is 100% complementary to at least 21 consecutive nucleotides of the polynucleotide sequence of the first expression cassette or an mRNA encoded by the polynucleotide sequence of the first expression cassette.

20. The recombinant DNA construct of claim 1 , wherein the amino acid sequence is at least 95% identical to SEQ ID NO: 2.

21. The recombinant DNA construct of claim 1 , wherein the amino acid sequence is 100% identical to SEQ ID NO: 2.

22. The recombinant DNA construct of claim 1 , wherein the first plant expressible promoter comprises a polynucleotide sequence that is 100% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 31, 32, and 48.

23. The recombinant DNA construct of claim 1 , wherein the second plant expressible promoter comprises a polynucleotide sequence that is 100% identical to SEQ ID NO: 49.

Assignees

• Monsanto Technology Llc

Inventors

• Brower-Toland Brent
• Dai Shunhong
• Gabbert Karen
• Goldshmidt Alexander
• Howell Miya
• Mcdill Brad
• Ovadya Dan
• Savidge Beth
• Sharma Vijay

Classifications

• C12N15/827 Primary

  Flower development or morphology, e.g. flowering promoting factor [FPF] · CPC title

• C12N15/8218

  Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS] · CPC title

• C07K14/415

  from plants · CPC title

• C12N15/8261

  with agronomic (input) traits, e.g. crop yield · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title