Biocatalytic microcapsules for catalyzing gas conversion

Patent metadata

Field	Value
Publication number	US-11542531-B2
Application number	US-201715411337-A
Country	US
Kind code	B2
Filing date	Jan 20, 2017
Priority date	Jan 21, 2016
Publication date	Jan 3, 2023
Grant date	Jan 3, 2023

Abstract

According to one embodiment, a microcapsule for selective catalysis of gases, the microcapsule comprising: a polymeric shell permeable to one or more target gases; and at least one biocatalyst disposed in an interior of the polymeric shell. In more embodiments, methods of forming such microcapsules include: emulsifying at least one biocatalyst in a polymer precursor mixture; emulsifying the polymer precursor mixture in an aqueous carrier solution; crosslinking one or more polymer precursors of the polymer precursor mixture to form a plurality of microcapsules each independently comprising: a polymeric shell permeable to one or more target gases; and at least one biocatalyst disposed in an interior of the polymeric shell. In further embodiments, corresponding methods of using the inventive microcapsules for catalyzing one or more target gases using include: exposing a plurality of the biocatalytic microcapsules to the one or more target gases.

First claim

What is claimed is:

1. A microcapsule for selective catalysis of gases, the microcapsule comprising:
a polymeric shell permeable to one or more target gases; and
at least one biocatalyst disposed in an interior of the polymeric shell, the at least one biocatalyst being operable to convert a C1-C3 hydrogen-comprising target gas to a product, wherein the C1-C3 hydrogen-comprising target gas is selected from the group consisting of: methane, ethane, ethylene, propane, and propylene.

2. The microcapsule as recited in claim 1 , wherein the at least one biocatalyst comprises one or more enzymes configured to catalyze the C1-C3 hydrogen-comprising target gas.

3. The microcapsule as recited in claim 1 , wherein the at least one biocatalyst includes reconstituted whole cells.

4. The microcapsule as recited in claim 1 , the at least one biocatalyst comprising one or more biocatalytic components selected from the group consisting of: one or more cell membrane fragments; and one or more cytosolic cell components.

5. The microcapsule as recited in claim 1 , wherein the at least one biocatalyst includes live cells.

6. The microcapsule as recited in claim 1 , wherein the at least one biocatalyst is dispersed throughout the interior of the polymeric shell.

7. The microcapsule as recited in claim 1 , wherein the polymeric shell is permeable to products of catalyzing and/or converting the C1-C3 hydrogen-comprising target gas.

8. The microcapsule as recited in claim 1 , comprising a buffer disposed in the interior of the polymeric shell, wherein the at least one biocatalyst is suspended in the buffer.

9. The microcapsule as recited in claim 1 , wherein the at least one biocatalyst selectively oxidizes the C1-C3 hydrogen-comprising target gas to form the product.

10. A product comprising a plurality of microcapsules for selective catalysis of gases, each microcapsule comprising:
a polymeric shell permeable to one or more target gases;
at least one biocatalyst disposed in an interior of the polymeric shell, the at least one biocatalyst comprising one or more biocatalytic components selected from the group consisting of: live cells, one or more cell membrane fragments, one or more cytosolic cell components, and reconstituted whole cells; and
a buffer disposed in the interior of the polymeric shell,
wherein the at least one biocatalyst is suspended in the buffer.

11. The product as recited in claim 10 , wherein the buffer comprises a reducing agent.

12. The product as recited in claim 2 , wherein the at least one biocatalyst comprises one or more enzymes configured to catalyze the one or more target gases.

13. The product as recited in claim 10 , wherein the at least one biocatalyst being operable to convert a C1-C3 hydrogen-comprising target gas to a product, wherein the C1-C3 hydrogen-comprising target gas is selected from the group consisting of: methane, ethane, ethylene, propane, and propylene.

14. The product as recited in claim 10 , wherein the microcapsules are arranged in a polymeric network in a configuration selected from the group consisting of: a microcapsule-embedded mesh; a packet containing the microcapsules; and a microcapsule-embedded adhesive.

15. The product as recited in claim 14 , wherein the polymeric network comprises at least one polymer material and at least one inorganic material.

16. The product as recited in claim 14 , wherein the polymeric network comprises at least a two phase polymer network.

17. The product as recited in claim 14 , wherein the microcapsules are embedded in the polymeric network.

18. The product as recited in claim 14 , wherein the polymeric shell includes a block polymer/copolymer network.

19. A product, comprising a plurality of the microcapsules as recited in claim 1 , wherein the microcapsules are arranged in a configuration selected from the group consisting of: a packed bed; a moving bed; a fluidized bed; a microcapsule-embedded mesh; a packet containing the microcapsules; and a microcapsule-embedded adhesive.

20. A microcapsule for selective catalysis of gases, the microcapsule comprising:
a polymeric shell permeable to one or more target gases; and
at least one biocatalyst disposed in an interior of the polymeric shell, wherein the at least one biocatalyst comprises one or more biocatalytic components selected from the group consisting of: one or more cell membrane fragments; one or more cytosolic cell components; live cells; and reconstituted whole cells.

21. The microcapsule as recited in claim 20 , wherein the at least one biocatalyst selectively oxidizes a C1-C3 hydrogen-comprising target gas to form a product.

Assignees

• L Livermore Nat Security Llc

Inventors

• Baker Sarah
• Stolaroff Joshuah K
• Ye Congwang

Classifications

• C12N11/082

  obtained by reactions only involving carbon-to-carbon unsaturated bonds · CPC title

• C12P7/04 Primary

  acyclic · CPC title

• C12N11/084

  Polymers containing vinyl alcohol units · CPC title

• B01J31/06

  containing polymers {(organometallic polymers B01J31/123; polymer-bound organometallic complexes B01J31/165; coordination polymers B01J31/1691)} · CPC title

• C12N11/089

  obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds · CPC title