Biologicals and their use in plants
US-10932471-B2 · Mar 2, 2021 · US
Method of preparation of microsclerotia of Beauveria bassiana
US11533916B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11533916-B2 |
| Application number | US-202016811381-A |
| Country | US |
| Kind code | B2 |
| Filing date | Mar 6, 2020 |
| Priority date | Mar 8, 2019 |
| Publication date | Dec 27, 2022 |
| Grant date | Dec 27, 2022 |
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- Title
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Abstract
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Disclosed is a bioinsecticide formulation comprising highly resistant Beauveria bassiana microsclerotia and to its use for insect control, in particular for control of insects in grain silos, and to a method of preparation of said formulation. The microsclerotia are obtained in a culture medium comprising an iron (II) compound at a concentration of approximately 0.2 g/L.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method to prepare an isolated microsclerotium of Beauveria bassiana , comprising: incubating the microsclerotium at 26° C. for 4 days with an agitation rate of 250 rpm in a culture medium comprising 0.4 g KH 2 PO 4 , 1.4 g Na 2 HPO 4 , 0.6 g MgSO 4 .7H 2 O, 1.0 g KCl, 0.7 g NH 4 NO 3 .7H 2 O, 0.2 g FeSO 4 .7H 2 O, 10 g glucose, and 5 g yeast extract in 1 L of distilled water. 2. The method according to claim 1 , further comprising: culturing Beauveria bassiana fungi to obtain a conidium, harvesting the conidium, preparing conidial suspensions, and isolating the microsclerotium. 3. The method according to claim 2 , wherein the carbon:nitrogen ratio of said culture medium is equal to or greater than 12.5:1.
Assignees
Inventors
Classifications
- A01N63/30Primary
Microbial fungi; Substances produced thereby or obtained therefrom · CPC title
- A01N65/00Primary
Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof (containing compounds of determined constitution A01N27/00 - A01N59/00) · CPC title
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- What does patent US11533916B2 cover?
- Disclosed is a bioinsecticide formulation comprising highly resistant Beauveria bassiana microsclerotia and to its use for insect control, in particular for control of insects in grain silos, and to a method of preparation of said formulation. The microsclerotia are obtained in a culture medium comprising an iron (II) compound at a concentration of approximately 0.2 g/L.
- Who is the assignee on this patent?
- Ypf Tecnologia Sa, Consejo Nacional De Investigaciones Cientificas Y Tecn Conicet, Univ Nacional De La Plata Unlp
- What technology area does this patent fall under?
- Primary CPC classification A01N63/30. Mapped technology areas include Human Necessities.
- When was this patent published?
- Publication date Tue Dec 27 2022 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 4 related publications on this page (citations in our corpus or others sharing the same primary CPC).