Biologicals and their use in plants

Patent metadata

Field	Value
Publication number	US-10932471-B2
Application number	US-201815949348-A
Country	US
Kind code	B2
Filing date	Apr 10, 2018
Priority date	Oct 12, 2015
Publication date	Mar 2, 2021
Grant date	Mar 2, 2021

Abstract

Entomopathogenic fungal strains, compositions, and methods of using the strains for reducing overall insect damage.

First claim

What is claimed is:

1. A composition comprising an agriculturally acceptable carrier, a plant or a plant part, and a fungal entomopathogen selected from the group consisting of Metarhizium robertsii 15013-1 (deposited under NRRL accession number 67073), Metarhizium robertsii 23013-3 (deposited under NRRL accession number 67075), and Metarhizium anisopliae 3213-1 (deposited under NRRL accession number 67074), wherein the fungal entomopathogen is applied to the plant or plant part, and wherein the composition comprises at least 1×10 2 CFUs of the fungal entomopathogen.

2. The composition of claim 1 , wherein the composition further comprises a biocontrol agent selected from the group consisting of a bacteria, a fungus, a yeast, a protozoa, a virus, an entomopathogenic nematode, a botanical extract, a protein, a nucleic acid, a secondary metabolite, and an inoculant.

3. The composition of claim 1 , wherein the composition further comprises an agrochemically active compound selected from the group consisting of an insecticide, a fungicide, a bactericide, and a nematicide.

4. The composition of claim 3 , wherein the agrochemically active compound is a fungicide.

5. The composition of claim 4 , wherein the fungicide comprises a fungicide composition selected from the group consisting of azoxystrobin, thiabendazole, fludioxonil, metalaxyl, tebuconazole, prothioconazole, ipconazole, penflufen, and sedaxane.

6. The composition of claim 1 , wherein the composition further comprises a compound selected from the group consisting of a safener, a lipo-chitooligosaccharide, a triglucosamine lipoglycine salt, an isoflavone, and a ryanodine receptor modulator.

7. The composition of claim 1 , wherein the plant or the plant part is genetically modified or transgenic.

8. The composition of claim 7 , wherein the genetically modified plant part is a seed.

9. The composition of claim 7 , wherein the genetically modified plant part is a leaf.

10. The composition of claim 1 , wherein the fungal entomopathogen retains insecticidal activity in the presence of a fungicide or is resistant to a fungicide.

11. The composition of claim 1 , wherein the fungal entomopathogen comprises a spore.

12. The composition of claim 1 , wherein the fungal entomopathogen comprises a conidia.

13. The composition of claim 1 , wherein the fungal entomopathogen comprises a microsclerotia.

14. A method of increasing resistance of a plant to a plant pathogen, a pest, or an insect comprising inoculating a plant, a plant part, or an environment of a plant or a plant part with a composition comprising a fungal entomopathogen selected from the group consisting of Metarhizium robertsii 15013-1 (deposited under NRRL accession number 67073), Metarhizium robertsii 23013-3 (deposited under NRRL accession number 67075), and Metarhizium anisopliae 3213-1 (deposited under NRRL accession number 67074), and wherein the composition comprises at least 1×10 2 CFUs of the fungal entomopathogen.

15. The method of claim 14 , wherein the composition further comprises a biocontrol agent selected from the group consisting of a bacteria, a fungus, a yeast, a protozoa, a virus, an entomopathogenic nematode, a botanical extract, a protein, a nucleic acid, a secondary metabolite, and an inoculant.

16. The method of claim 14 , wherein the composition further comprises an agrochemically active compound selected from the group consisting of an insecticide, a fungicide, a bactericide, and a nematicide.

17. The method of claim 16 , wherein the agrochemically active compound is a fungicide.

18. The method of claim 17 , wherein the fungicide comprises a fungicide composition selected from the group consisting of azoxystrobin, thiabendazole, fludioxonil, metalaxyl, tebuconazole, prothioconazole, ipconazole, penflufen, and sedaxane.

19. The method of claim 14 , wherein the composition further comprises a compound selected from the group consisting of a safener, a lipo-chitooligosaccharide, a triglucosamine lipoglycine salt, an isoflavone, and a ryanodine receptor modulator.

20. The method of claim 14 , wherein the plant, the plant part, or the environment of the plant or the plant part further comprises a genetically modified or transgenic plant or plant part, or an environment of a genetically modified or transgenic plant or plant part.

21. The method of claim 14 , wherein the genetically modified plant part is a seed.

22. The method of claim 14 , wherein the genetically modified plant part is a leaf.

23. The method of claim 20 , wherein the genetically modified or transgenic plant or plant part comprises a Coleopteran insecticidal trait.

24. The method of claim 23 , wherein the Coleopteran insecticidal trait comprises a Bt trait, a silencing element or a non-Bt insecticidal protein.

25. The method of claim 24 , wherein the non-Bt insecticidal protein is selected from the group consisting of a plant-derived insecticidal protein, non-Bt bacterial/archeal-derived insecticidal protein, an animal-derived insecticidal protein, an AfIP-1A and/or AfIP-1B polypeptide, a PHI-4 polypeptide, a PIP-47 polypeptide, a PIP-72 polypeptide, a PtIP-50 polypeptide and a PtIP-65 polypeptide, a PtIP-83 polypeptide, and a PtIP-96 polypeptide.

26. The method of claim 24 , wherein the silencing element targets a gene selected from the group consisting of PAT3, RyanR, Sec23, Snf7, vATPase, a COPI α, β, or γ coatomer subunit, and RPS10.

27. The method of claim 24 , wherein the Bt trait comprises an event selected from the group consisting of event MON863, event MIR604, event 5307, event DAS-59122, event DP-4114, event MON 87411, and event MON88017.

28. The method of claim 14 , wherein the fungal entomopathogen comprises a spore.

29. The method of claim 14 , wherein the fungal entomopathogen comprises a conidia.

30. The method of claim 14 , wherein the fungal entomopathogen comprises a microsclerotia.

Assignees

• Pioneer Hi Bred Int
• Du Pont

Inventors

• Bruck Denny Joseph
• Burns Ii Frederick C
• Presnail James Kevin

Classifications

• A01N63/00

  Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates (containing compounds of determined constitution A01N27/00 - A01N59/00; unicellular algae A01N65/03) · CPC title

• A01N63/30 Primary

  Microbial fungi; Substances produced thereby or obtained therefrom · CPC title

• A01N43/78

  1,3-Thiazoles; Hydrogenated 1,3-thiazoles · CPC title

• A01N25/00

  Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application {, e.g. seed treatment or sequential application}; Substances for reducing the noxious effect of the active ingredients to organisms other than pests · CPC title

• A01N25/30

  characterised by the surfactants · CPC title