Lactase enzymes with improved properties at acidic ph
US-2021348147-A1 · Nov 11, 2021 · US
Lactase enzymes with improved properties
US11525129B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11525129-B2 |
| Application number | US-201816604129-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 11, 2018 |
| Priority date | Apr 11, 2017 |
| Publication date | Dec 13, 2022 |
| Grant date | Dec 13, 2022 |
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- Title
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Abstract
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Disclosed are peptides and dimeric peptides exhibiting beta-galactosidase enzyme activity, as well as methods for producing a dairy product using them, and dairy products made by such methods. In some embodiments, the peptides exhibit beta-galactosidase enzyme activity at low and/or high temperatures.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method for producing a dairy product, comprising treating a milk-based substrate comprising lactose with a peptide selected from peptides having the amino acid sequence of any one of SEQ ID NO:1, SEQ ID NO:22, or SEQ ID NO:32, and variants thereof having from 1 to 5 amino acid substitutions, additions or deletions relative thereto, wherein said treating takes place at a pH of 4 to 7 and at a temperature selected from (i) a temperature of not more than 25° C. and (ii) a temperature of between 40° C. and 100° C. wherein the peptide exhibits beta-galactosidase enzyme activity at the temperature and reduces lactose content of the substrate. 2. The method according to claim 1 , wherein said treating takes place at a temperature of not more than 25° C. 3. The method according to claim 1 , wherein said dairy product is fermented milk product and said treating is performed during or prior to fermentation. 4. The method according to claim 1 , which method does not require the addition of further enzyme after fermentation. 5. The method according to claim 1 , wherein said dairy product is a fermented milk product and said treating is performed immediately following fermentation. 6. The method according to claim 1 , wherein said dairy product is fresh milk and said treating is performed prior to, in conjunction with, or immediately following pasteurization. 7. The method according to claim 1 , wherein said dairy product is ultra-heat treated (UHT) milk and said treating is performed prior to, in conjunction with, or immediately following ultra-heat treatment. 8. The method according to claim 1 , wherein said treating takes place at a temperature of between 40° C. and 100° C. 9. The method according to claim 1 , wherein the method comprises treating said milk-based substrate with said peptide, wherein said peptide has the amino acid sequence of SEQ ID NO:1 or a variant thereof having from 1 to 5 amino acid substitutions relative thereto. 10. The method according to claim 9 , wherein said peptide has the amino acid sequence of SEQ ID NO:1. 11. The method according to claim 1 , wherein the method comprises treating said milk-based substrate with said peptide, wherein said peptide has the amino acid sequence of SEQ ID NO:22 or a variant thereof having from 1 to 5 amino acid substitutions relative thereto, wherein said peptide exhibits beta-galactosidase enzyme activity at 72° C. 12. The method according to claim 11 , wherein said peptide has the amino acid sequence of SEQ ID NO:22. 13. The method according to claim 1 , wherein the method comprises treating said milk-based substrate with said peptide, wherein said peptide has the amino acid sequence of SEQ ID NO:32 or a variant thereof having from 1 to 5 amino acid substitutions relative thereto. 14. The method according to claim 9 , wherein said peptide has the amino acid sequence of SEQ ID NO:32.
Assignees
Inventors
Classifications
Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase · CPC title
- C07K14/195Primary
from bacteria · CPC title
- C12N9/2471Primary
Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase · CPC title
Enzymes · CPC title
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Frequently asked questions
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- What does patent US11525129B2 cover?
- Disclosed are peptides and dimeric peptides exhibiting beta-galactosidase enzyme activity, as well as methods for producing a dairy product using them, and dairy products made by such methods. In some embodiments, the peptides exhibit beta-galactosidase enzyme activity at low and/or high temperatures.
- Who is the assignee on this patent?
- Chr Hansen As
- What technology area does this patent fall under?
- Primary CPC classification C07K14/195. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Dec 13 2022 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 9 related publications on this page (citations in our corpus or others sharing the same primary CPC).