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Method for producing 3-fucosyllactose using Corynebacterium glutamicum
US11512318B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11512318-B2 |
| Application number | US-201816606921-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 20, 2018 |
| Priority date | Apr 21, 2017 |
| Publication date | Nov 29, 2022 |
| Grant date | Nov 29, 2022 |
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Abstract
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Disclosed is a method for producing 3-fucosyllactose using a wild Corynebacterium glutamicum strain. In addition, using the Corynebacterium glutamicum strain, which is a GRAS strain, 3-fucosyllactose can be produced at a high concentration, high yield and high productivity.
First claim
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The invention claimed is: 1. A method of producing 3-fucosyllactose comprising culturing in a medium supplemented with lactose a recombinant Corynebacterium glutamicum transformed to express α-1, 3-fucosyltransferase, transformed to express GDP-D-mannose-4, 6-dehydratase, transformed to express GDP-L-fucose synthase, and transformed to express lactose permease, and isolating 3-fucosyllactose from the medium, wherein the recombinant Corynebacterium glutamicum has phosphomannomutase and GTP-mannose-1-phosphate guanylyltransferase. 2. The method according to claim 1 , wherein the medium further comprises glucose. 3. The method according to claim 2 , wherein the production of the 3-fucosyllactose is carried out by batch culture or fed-batch culture comprising further supplying glucose or lactose. 4. The method according to claim 1 , wherein the recombinant Corynebacterium glutamicum is transformed to overexpress phosphomannomutase, and is transformed to overexpress GTP-mannose-1-phosphate guanylyltransferase.
Assignees
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Classifications
Phosphomannomutase (5.4.2.8) · CPC title
Preparation of compounds containing saccharide radicals (ketoaldonic acids C12P7/58) · CPC title
Oligosaccharide-transporting ATPase (3.6.3.18) · CPC title
Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds · CPC title
Isomerases (5.) · CPC title
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- What does patent US11512318B2 cover?
- Disclosed is a method for producing 3-fucosyllactose using a wild Corynebacterium glutamicum strain. In addition, using the Corynebacterium glutamicum strain, which is a GRAS strain, 3-fucosyllactose can be produced at a high concentration, high yield and high productivity.
- Who is the assignee on this patent?
- Seoul Nat Univ R&Db Foundation, Advanced Protein Tech Corp
- What technology area does this patent fall under?
- Primary CPC classification C12N9/0006. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Nov 29 2022 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).