Separation substrate, cell separation filter, and method for producing platelet

Patent metadata

Field	Value
Publication number	US-11512275-B2
Application number	US-201916659242-A
Country	US
Kind code	B2
Filing date	Oct 21, 2019
Priority date	May 12, 2017
Publication date	Nov 29, 2022
Grant date	Nov 29, 2022

Abstract

An object of the present invention is to provide a separation substrate having a high megakaryocyte blocking rate and a high platelet permeation rate, and a cell separation filter and a method for producing a platelet which use the same. The separation substrate of the present invention is a separation substrate including non-woven fabric for separating a platelet from a cell suspension containing a megakaryocyte and the platelet, in which an average pore diameter of the separation substrate is 2.0 μm to 15.0 μm, and a thickness of the separation substrate is 10 μm to 500 μm.

First claim

What is claimed is:

1. A method for producing a platelet, comprising:
a production step of producing a platelet from a megakaryocyte;
a contact step of contacting a separation substrate with a culture solution containing at least the megakaryocyte and the platelet;
and
a recovery step of recovering, as a filtrate, a culture solution containing a produced platelet separated by the separation substrate after the contact step;
wherein the separation substrate comprises non-woven fabric including cellulose acylate containing two or more acyl groups for separating a megakaryocyte and a platelet from a cell suspension containing the megakaryocyte and the platelet, blocking the megakaryocyte at a rate of 90% or more, and recovering the platelets,
an average pore diameter of the separation substrate is 2.0 μm to 15.0 μm, and
a thickness of the separation substrate is 10 μm to 500 μm.

2. The method for producing a platelet according to claim 1 , wherein air permeability of the separation substrate is 2 cm 3 /cm 2 /s to 40 cm 3 /cm 2 /s.

3. The method for producing a platelet according to claim 1 , wherein air permeability of the separation substrate is 5 cm 3 /cm 2 /s to 30 cm 3 /cm 2 /s.

4. The method for producing a platelet according to claim 1 , wherein an average fiber diameter of a fiber forming the non-woven fabric is 800 nm to 1900 nm.

5. The method for producing a platelet according to claim 1 , wherein a void volume of the separation substrate is 40% to 90%.

6. The method for producing a platelet according to claim 1 , wherein a void volume of the separation substrate is more than 50% and less than 85%.

7. The method for producing a platelet according to claim 1 , wherein the platelet permeation rate of the separation substrate is 80% or more.

8. The method for producing a platelet according to claim 1 , wherein the non-woven fabric is a non-woven fabric produced by an electrospinning method.

9. The method for producing a platelet according to claim 1 , wherein the non-woven fabric is a single-layer non-woven fabric.

Assignees

• Fujifilm Corp

Inventors

• Takei Toshiki
• Yamada Tadanori
• Takegami Ryuta
• Kaminaga Kuniyuki
• FUKUNAGA Akihito
• Kanemura Kazuhide

Classifications

• B01D2239/1233

  Fibre diameter · CPC title

• B01D39/18

  the material being cellulose or derivatives thereof ({cork or peat B01D39/1646} ; making filter paper D21F11/14) · CPC title

• B01D2239/1258

  Permeability · CPC title

• C12N5/0068

  General culture methods using substrates (for specific animal cell type C12N5/06) · CPC title

• B01D2239/1216

  Pore size · CPC title