Automated immunoassay

Patent metadata

Field	Value
Publication number	US-11480565-B2
Application number	US-202016900577-A
Country	US
Kind code	B2
Filing date	Jun 12, 2020
Priority date	Jun 12, 2020
Publication date	Oct 25, 2022
Grant date	Oct 25, 2022

Abstract

An instrument for detecting signal from a biological sample includes a pipettor module configured to hold a plurality of pipettes in respective pipette positions, to hold liquid in one or more pipette tips, and to pipette liquid in and out of the one or more pipette tips. Each of the one or more pipette tips has a pipette tip point. The instrument further includes one or more magnets positioned such that each of the one or more pipette tips is adjacent one of the one or more magnets.

First claim

What is claimed is:

1. An instrument for detecting signal from a biological sample, the instrument comprising:
a pipettor module configured to hold a plurality of pipettes in respective pipette positions, each of the pipette positions having an associated pipette tip position, to hold liquid in one or more pipette tips, and to pipette liquid in and out of the one or more pipette tips, each of the one or more pipette tips having a pipette tip point;
one or more magnets positioned such that each of the one or more pipette tips is adjacent to one of the one or more magnets;
an optical detection module positioned to detect an optical signal from particles magnetically fixed to respective sides of the one or more pipette tips by the one or more magnets; and
one or more mirrors positioned such that the optical signal from each of the one or more pipette tips is reflected from one of the one or more mirrors and directed at an angle substantially parallel to the orientation of the pipette tips and in the direction of the pipette tip points.

2. The instrument of claim 1 , wherein the instrument comprises exactly one magnet for each pipette tip position.

3. The instrument of claim 1 , wherein the optical signals are reflected directly from the one or more mirrors to the optical detection module without any further reflections.

4. The instrument of claim 1 , wherein the one or more mirrors are one or more first mirrors, the instrument further comprising:
a second mirror positioned under the pipette tip points that redirects the optical signal from the one or more first mirrors to the optical detection module.

5. The instrument of claim 4 , wherein the instrument comprises two or more second mirrors, each of the two or more second mirrors reflecting optical signal from a subset of the pipette tips.

6. The instrument of claim 4 , wherein the optical detection module comprises a camera positioned to receive optical signal reflected from the second mirror.

7. The instrument of claim 4 , wherein the optical detection module has only one camera positioned to receive optical signal originating from each of the one or more pipette tips.

8. The instrument of claim 4 , wherein the optical detection module has a plurality of cameras, wherein each of the plurality of cameras is positioned to receive optical signal originating from only a subset of the pipette tips.

9. The instrument of claim 4 , wherein there are fewer first mirrors than pipette tip positions, and at least some of the first mirrors reflect optical signal from more than one of the one or more pipette tips.

10. The instrument of claim 1 , comprising a plurality of pipette tips disposed in a line or in a two-dimensional array, wherein the pipettor module is configured to hold and pipette liquid in and out of the one or more of pipette tips simultaneously.

11. An instrument for detecting signal from a biological sample, the instrument comprising:
a pipettor module configured to hold a plurality of pipettes in respective pipette positions, each of the pipette positions having an associated pipette tip position, to hold liquid in one or more pipette tips, and to pipette liquid in and out of the one or more pipette tips, each of the one or more pipette tips having a pipette tip point;
one or more magnets positioned such that each of the one or more pipette tips is adjacent to one of the one or more magnets;
a magnet module, wherein the magnet module comprises one or more spring clips, one spring clip respectively for each of the one or more magnets, the one or more spring clips positioned to hold the one or more magnets against the pipette tips; and
a magnet module coupling station where the magnet module is coupled to the pipettor module, the magnet module coupling station comprising one or more posts positioned to deflect the one or more magnets away from the one or more pipettes against the action of the one or more spring clips during coupling of the magnet module to the pipettor module.

12. The instrument of claim 11 , further comprising a motorized mechanism configured to move the pipettor module into and out of the magnet module coupling station to automatically couple the magnet module to the pipettor module.

13. The instrument of claim 12 , further comprising:
one or more optical detection modules positioned to detect an optical signal from particles magnetically fixed to respective sides of the one or more pipette tips by the one or more magnets; and
one or more mirrors positioned such that the optical signal from each of the one or more pipette tips is reflected from one of the one or more mirrors and directed at an angle substantially parallel to the orientation of the pipette tips and in the direction of the pipette tip points, the one or more mirrors disposed at a detection station;
wherein the motorized mechanism is further configured to move the coupled pipettor module and magnet module to the detection station.

14. The instrument of claim 12 , further comprising a reagent loading station, and wherein the motorized mechanism is further configured to move the pipettor module to the reagent loading station.

15. The instrument of claim 14 , further comprising a shaker for agitating liquids present at the reagent loading station.

Assignees

• Bio Rad Laboratories Inc

Inventors

• Nimri Shai
• Uri Yochanan
• Ran Boaz

Classifications

• B01L3/5085

  for multiple samples, e.g. microtitration plates · CPC title

• G01N33/5302 Primary

  Apparatus specially adapted for immunological test procedures · CPC title

• G01N35/10

  Devices for transferring samples {or any liquids} to, in, or from, the analysis apparatus, e.g. suction devices, injection devices {(G01N35/0099 takes precedence)} · CPC title

• G01N2035/0477

  Magnetic · CPC title

• G01N2035/103

  using disposable tips · CPC title