Algal mutants with increased lipid productivity
US-2017121742-A1 · May 4, 2017 · US
US11464244B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11464244-B2 |
| Application number | US-201515516022-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 22, 2015 |
| Priority date | Oct 2, 2014 |
| Publication date | Oct 11, 2022 |
| Grant date | Oct 11, 2022 |
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According to the invention, it was found that, surprisingly, a feedstuff having a very high abrasion resistance and very high water stability can be obtained when a PUFA-containing biomass having a high sulphate content is used for producing the feedstuff.
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The invention claimed is: 1. A PUFA (polyunsaturated fatty acid)-containing feedstuff, comprising 2 to 22% by weight of dry biomass, wherein: a) said biomass is obtained by the fermentation of Labyrinthulea cells; b) said biomass comprises 25-60 g/kg of sulphate by weight of dry biomass; c) the 25-60 g/kg of sulphate in said dry biomass is from sulphate present during the fermentation of said Labyrinthulea cells; and wherein the PUFA-containing feedstuff is characterized by an abrasion resistance, based on its Pellet Durability Index (PDI), of at least 91% and a water stability of at least 96%. 2. The PUFA-containing feedstuff of claim 1 , wherein the Labyrinthulea cells are in the family Thraustochytriaceae. 3. The PUFA-containing feedstuff of claim 2 , wherein said feedstuff contains biomass in an amount of 9 to 20% by weight. 4. The PUFA-containing feedstuff of claim 2 , wherein the Labyrinthulea cells in the family Thraustochytriaceae are of the species Schizochytrium or Aurantiochytrium. 5. The PUFA-containing feedstuff of claim 1 , and wherein said feedstuff is coated with oil in an amount of 3-18% by weight. 6. The PUFA-containing feedstuff of claim 5 , wherein said feedstuff is coated with 7-13% by weight of a coating that is a vegetable oil. 7. The PUFA-containing feedstuff of claim 1 , wherein the Labyrinthulea biomass is present in an amount of 11-18% by weight. 8. The PUFA-containing feedstuff of claim 1 , wherein the Labyrinthulea biomass is present in an amount of 4 to 20% by weight and said feedstuff is coated with oil in an amount of 5-15% by weight. 9. The PUFA-containing feedstuff of claim 1 , wherein the abrasion resistance is at least 94%. 10. The PUFA-containing feedstuff of claim 9 , wherein the water stability is at least 98%. 11. The PUFA-containing feedstuff of claim 1 , wherein the water stability is at least 98%. 12. The PUFA-containing feedstuff of claim 1 , further comprising one or more of the following: a) a polyunsaturated fatty acid (PUFA) content of 0.8 to 8% by weight; b) an omega-3 fatty acid content of 0.8 to 8% by weight; and c) a DHA content of 0.1 to 4.0% by weight. 13. The PUFA-containing feedstuff of claim 1 , comprising the following properties: a) a total protein content of 33 to 67% by weight; b) a total fat content of 5 to 25% by weight; c) a total starch content of at most 25% by weight; and wherein the PUFA-containing feedstuff of claim 1 , further comprises one or more of the following: e) a polyunsaturated fatty acid (PUFA) content of 0.8 to 8% by weight; f) an omega-3 fatty acid content of 0.8 to 8% by weight; and/or g) a DHA content of 0.1 to 4.0% by weight. 14. The PUFA-containing feedstuff of claim 1 , wherein said biomass comprises 25-40 g/kg of sulphate by weight of dry biomass. 15. A process for producing the PUFA-containing feedstuff of claim 1 , wherein said process comprises extruding a PUFA-containing biomass having a sulphate content of 25 to 60 g per kilogram of biomass together with other feedstuff components. 16. The process of claim 15 , wherein the extrudate is dried and then loaded with oil. 17. The process of claim 16 , wherein extrusion and drying is followed by coating of the extrudate with oil in an amount of 3 to 17% by weight. 18. The process of claim 15 , wherein the extrusion is carried out at an energy input of 12-28 Wh/kg. 19. The process of claim 15 , wherein the composition used for extrusion comprises: a) a total protein content of 33 to 67% by weight; b) a total fat content of 5 to 25% by weight; c) a total starch content of at most 25% by weight. 20. The process of claim 19 , wherein the composition used for extrusion further comprises one or more of the following: e) a PUFA content of 0.8 to 8% by weight; f) an omega-3 fatty acid content of 0.8 to 8% by weight; g) a DHA content of 0.1 to 4.0% by weight. 21. A method for farming animals comprising feeding said animals the PUFA-containing feedstuff of claim 1 .
for aquatic animals, e.g. fish, crustaceans or molluscs · CPC title
Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions (A23K10/12 takes precedence) · CPC title
by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass · CPC title
Sugars; Polysaccharides · CPC title
by encapsulating; by coating · CPC title
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