Stacked herbicide tolerance event 8264.44.06.1, related transgenic soybean lines, and detection thereof

Patent metadata

Field	Value
Publication number	US-11425906-B2
Application number	US-202117224694-A
Country	US
Kind code	B2
Filing date	Apr 7, 2021
Priority date	Dec 3, 2010
Publication date	Aug 30, 2022
Grant date	Aug 30, 2022

Abstract

This invention relates in part to soybean event pDAB8264.44.06.1 and includes a novel expression cassettes and transgenic inserts comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding and herbicide tolerant plants. In some embodiments, the event sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to endpoint TaqMan PCR assays for the detection of Event pDAB8264.44.06.1 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.

First claim

The invention claimed is:

1. A method for identifying Event pDAB8264.44.06.1 in a sample, said method comprising detecting a junction sequence of Event pDAB8264.44.06.1, as present in seed deposited under ATCC Accession number PTA-11336, with a probe or at least one primer that specifically binds with or amplifies said junction sequence, said junction sequence comprising residues 561-580 of SEQ ID NO: 14, or residues 211-230 of SEQ ID NO: 15.

2. The method of claim 1 , said method further comprising amplifying a DNA fragment from a nucleic acid present in said sample using a polymerase chain reaction with at least a first primer and a second primer, wherein said Event pDAB8264.44.06.1 comprises SEQ ID NO:27 and wherein said first primer specifically binds an insert sequence within said SEQ ID NO: 27, or the complement thereof, and said second primer specifically binds a sequence within a flanking sequence of SEQ ID NO:27, or the complement thereof, wherein said flanking sequence is selected from the group consisting of SEQ ID NO: 1 and SEQ ID NO:2.

3. A kit for performing the method of claim 1 , said kit comprising said probe and said at least one primer.

4. The method of claim 2 , wherein said first primer specifically binds an insert sequence within said SEQ ID NO: 27, and said second primer specifically binds a sequence within a flanking sequence of SEQ ID NO: 27, wherein said flanking sequence is selected from the group consisting of SEQ ID NO: 1 and SEQ ID NO: 2.

5. The method of claim 1 , wherein said probe comprises SEQ ID NO: 20.

6. The method of claim 2 , wherein said probe comprises SEQ ID NO: 20.

7. The method of claim 4 , wherein said probe comprises SEQ ID NO: 20.

8. The method of claim 1 , wherein said at least one primer comprises a sequence selected from the group consisting of SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 21, and SEQ ID NO: 22.

9. The method of claim 2 , wherein said primers comprise a sequence selected from the group consisting of SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 21, and SEQ ID NO: 22.

10. The method of claim 4 , wherein said primers comprise a sequence selected from the group consisting of SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 21, and SEQ ID NO: 22.

11. The method of claim 1 , wherein said sample is a DNA sample and is obtained from a soybean plant in a field.

12. The method of claim 1 , wherein said at least one primer comprises a sequence selected from the group consisting of SEQ ID NO: 18 and SEQ ID NO: 19.

13. The method of claim 2 , wherein said first primer comprises SEQ ID NO: 18 and said second primer comprises SEQ ID NO: 19.

14. The method of claim 4 , wherein said first primer comprises SEQ ID NO: 18 and said second primer comprises SEQ ID NO: 19.

15. The method of claim 1 , wherein said at least one primer comprises a sequence selected from the group consisting of SEQ ID NO: 21 and SEQ ID NO: 22.

16. The method of claim 2 , wherein said first primer comprises SEQ ID NO: 21 and said second primer comprises SEQ ID NO: 22.

17. The method of claim 4 , wherein said first primer comprises SEQ ID NO: 21 and said second primer comprises SEQ ID NO: 22.

18. The kit of claim 3 , wherein said probe comprises SEQ ID NO: 20.

19. The kit of claim 3 , wherein said at least one primer comprises a sequence selected from the group consisting of SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 21, and SEQ ID NO: 22.

20. The kit of claim 3 , wherein said at least one primer comprises a sequence selected from the group consisting of SEQ ID NO: 18 and SEQ ID NO: 19.

21. The kit of claim 3 , wherein said at least one primer comprises a sequence selected from the group consisting of SEQ ID NO: 21 and SEQ ID NO: 22.

22. The kit of claim 3 , wherein said probe comprises SEQ ID NO: 20 and said at least one primer comprises a sequence selected from the group consisting of SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 21, and SEQ ID NO: 22.

23. The kit of claim 3 , wherein said probe comprises SEQ ID NO: 20 and said at least one primer comprises a sequence selected from the group consisting of SEQ ID NO: 18 and SEQ ID NO: 19.

24. The kit of claim 3 , wherein said probe comprises SEQ ID NO: 20 and said at least one primer comprises a sequence selected from the group consisting of SEQ ID NO: 21 and SEQ ID NO: 22.

Assignees

• Dow Agrosciences Llc
• Ms Technologies Llc

Inventors

• Cui Yunxing C
• Hoffman Thomas
• Zhou Ning
• Novak Stephen N
• Colon Julissa
• Parkhurst Dawn M
• Toledo Sandra G
• Wright Terry R
• Russell Sean M
• Held Bruce
• Sekar Vaithilingam

Classifications

• C12N15/8275

  Glyphosate · CPC title

• C12Q2600/156

  Polymorphic or mutational markers · CPC title

• C12N9/93 Primary

  Ligases (6) · CPC title

• C12N15/8274

  for herbicide resistance · CPC title

• C12N9/1092 Primary

  3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase · CPC title