Stacked herbicide tolerance event 8264.44.06.1, related transgenic soybean lines, and detection thereof

Patent metadata

Field	Value
Publication number	US-9540655-B2
Application number	US-201113991246-A
Country	US
Kind code	B2
Filing date	Dec 2, 2011
Priority date	Dec 3, 2010
Publication date	Jan 10, 2017
Grant date	Jan 10, 2017

Abstract

This invention relates in part to soybean event pDAB8264.44.06.1 and includes a novel expression cassettes and transgenic inserts comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding and herbicide tolerant plants. In some embodiments, the event sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to endpoint TaqMan PCR assays for the detection of Event pDAB8264.44.06.1 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.

First claim

The invention claimed is:

1. A transgenic soybean plant cell comprising a genome comprising SEQ ID NO: 27 wherein said SEQ ID NO: 27 comprises a first sequence represented by SEQ ID NO:16 and a second sequence represented by SEQ ID NO:17, wherein said first sequence spans the 5′ integration junction of said polynucleotide segment and said second sequence spans the 3′ integration junction of said polynucleotide segment.

2. A soybean seed comprising a genome comprising Event pDAB8264.44.06.1, wherein said soybean seed is present in representative seed deposited with American Type Culture Collection (ATCC) under Accession No. PTA-11336.

3. A soybean seed comprising the transgenic soybean plant cell of claim 1 , said seed comprising said polynucleotide segment.

4. A soybean plant produced by growing the seed of claim 2 , said plant comprising said Event pDAB8264.44.06.1.

5. A progeny plant of the soybean plant of claim 4 , said progeny plant comprising said Event pDAB8264.44.06.1.

6. A transgenic soybean plant comprising a plurality of cells of claim 1 .

7. The transgenic soybean plant of claim 6 , said plurality of cells further comprising an insect resistance gene.

8. The transgenic soybean plant of claim 6 wherein the plant is resistant to at least one herbicide selected from the group consisting of a phenoxyacetic acid herbicide, a phenoxybutanoic acid herbicide, a pyridyloxyalkanoic acid herbicide, a glyphosate herbicide, and a glufosinate herbicide.

9. A part of the soybean plant of claim 4 wherein said part is selected from the group consisting of pollen, an ovule, a flower, a shoot, a root, and a leaf, said part comprising said Event pDAB8264.44.06.1.

10. A plant cell comprising a genome comprising Event pDAB8264.44.06.1 as present in representative seeds deposited with American Type Culture Collection (ATCC) under Accession No. PTA-11336.

11. A method of controlling weeds in a field, said method comprising applying a herbicide selected from the group consisting of a phenoxyacetic acid, a phenoxybutanoic acid, a pyridyloxyalkanoic acid, a glyphosate, and a glufosinate to the field, and planting a seed of claim 2 in the field within 14 days of applying the herbicide.

12. The transgenic soybean plant of claim 6 , wherein the plant is from Glycine max.

13. A soybean plant produced by growing the soybean seed of claim 3 , said plant comprising said polynucleotide segment.

14. A part of the soybean plant of claim 13 wherein said part is selected from the group consisting of pollen, an ovule, a flower, a shoot, a root, and a leaf, said part comprising said polynucleotide segment.

15. A method of controlling weeds in a field, said method comprising applying at least one herbicide selected from the group consisting of a 2,4-dichlorophenoxyacetic acid, a glyphosate, and a glufosinate to the field, and planting a seed of claim 3 .

Assignees

• Cui Yunxing
• Hoffman Thomas
• Zhou Ning
• Novak Stephen N
• Colon Julissa
• Parkhurst Dawn
• Toledo Sandra
• Wright Terry
• Russell Sean
• Held Bruce
• Sekar Vaithilingam
• Dow Agrosciences Llc
• Ms Tech L L C

Inventors

• Cui Yunxing
• Hoffman Thomas
• Zhou Ning
• Novak Stephen N
• Colon Julissa
• Parkhurst Dawn
• Toledo Sandra
• Wright Terry
• Russell Sean
• Held Bruce
• Sekar Vaithilingam

Classifications

• C12Q2600/156

  Polymorphic or mutational markers · CPC title

• C12Y604/01002

  Acetyl-CoA carboxylase (6.4.1.2) · CPC title

• C12Q1/6895

  for plants, fungi or algae · CPC title

• C12Y205/01019

  3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase · CPC title

• C12N15/8275 Primary

  Glyphosate · CPC title