Thermoresponsive cell culture supports

Patent metadata

Field	Value
Publication number	US-11414645-B2
Application number	US-201916239671-A
Country	US
Kind code	B2
Filing date	Jan 4, 2019
Priority date	May 26, 2016
Publication date	Aug 16, 2022
Grant date	Aug 16, 2022

Abstract

The present invention relates to a cell culture support comprising a substrate and a polymeric blend layer bound to the substrate. The polymeric blend layer comprises at least one thermoresponsive polymer and at least one coupling agent. The coupling agent is a non-protein coupling agent that has functional thiol, ester, epoxy, or aldehyde groups. The cell culture support further includes cells supported by the polymeric blend layer, wherein the thermoresponsive polymer provides for temperature induced detachment of the cells and/or cell sheets.

First claim

What is claimed is:

1. A method of making a cell culture complex comprising:
providing a substrate;
blending at least one thermoresponsive polymer and at least one coupling agent having functional thiol, ester, epoxy, or aldehyde groups to provide a polymeric blend;
applying a thin film of said polymeric blend to the substrate to provide a polymeric blend layer on the substrate;
curing the polymeric blend layer on the substrate to provide a cell culture support such that the at least one coupling agent forms a three-dimensional multilayered network which entraps the at least one thermoresponsive polymer; and
depositing cells onto said cell culture support to form a cultured cell layer, wherein the cells may optionally further comprise medium, to provide a cell culture complex.

2. The method of claim 1 , wherein the cultured cell layer comprises cells further characterized as anchor dependent cells.

3. The method of claim 1 , wherein the cultured cell layer comprises cells further characterized as adhesive cells.

4. The method of claim 1 , wherein the cultured cell layer comprises cells selected from the group consisting of fibroblasts, myoblasts, myotube cells, corneal cells, vascular endothelial cells, smooth muscle cells, cardiomyocytes, dermal cells, epidermal cells, mucosal epithelial cells, mesenchymal stem cells, ES cells, iPS cells, osteoblasts, osteocytes, chondrocytes, fat cells, neurons, hair root cells, dental pulp stem cells, β-cells, hepatocytes, and combinations thereof.

5. The method of claim 1 , wherein the cultured cell layer comprises cells and medium.

6. The method of claim 1 , wherein the thin film of said polymeric blend is spin-coated onto the substrate.

7. The method of claim 1 , wherein the polymeric blend layer is devoid of adhesive proteins, plasma treatment, and e-beam treatment; and wherein the method of claim 1 further includes a step of detaching the cultured cell layer, and wherein the step of detaching is devoid of the use of proteolytic enzymes or mechanical agitations.

8. The method of claim 1 , wherein the step of blending further includes creating a first solution of the at least one coupling agent in ethanol, creating a second solution of the at least one thermoresponsive polymer in ethanol, and combining the first solution with the second solution to provide the polymeric blend.

9. The method of claim 1 , wherein the step of blending further includes creating a mixture of the at least one coupling agent and the at least one thermoresponsive polymer and dissolving the mixture in ethanol to provide the polymeric blend.

10. The method of claim 1 , wherein the at least one thermoresponsive polymer is selected from the group consisting of poly (N-isopropylacrylamide)(PNIPAAm), poly(N,N-diethylacrylamide) (PDEAAm), poly(N-vinlycaprolactam) (PVCL), poly[2-(dimethylamino)ethyl methacrylate] (PDMAEMA), poly(ethylene oxide) (PEO), 2-oxazoline (Pox), poly(2-ethyl-2-oxazoline), poly(2-cyclopropyl-2-oxazoline), poly(2-n-propyl-2-oxazoline), poly(2-isopropyl-2-oxazoline); poly(2-ethyl-oxazine), poly(2-n-propyl-oxazine), and combinations thereof.

Assignees

• Alghunaim Abdullah
• Newby Bi Min Zhang
• Univ Akron

Inventors

• Alghunaim Abdullah
• Newby Bi-min Zhang

Classifications

• C08K5/5435

  containing oxygen in a ring · CPC title

• C12N2539/10

  Coating allowing for selective detachment of cells, e.g. thermoreactive coating · CPC title

• C08K5/548

  containing sulfur {(C08K5/5477 takes precedence)} · CPC title

• C09D133/24

  Homopolymers or copolymers of amides or imides · CPC title

• C12N5/0068 Primary

  General culture methods using substrates (for specific animal cell type C12N5/06) · CPC title