Amylopectin-based cyclic glucan and method for processing the same

Patent metadata

Field	Value
Publication number	US-11396559-B2
Application number	US-202016875048-A
Country	US
Kind code	B2
Filing date	May 15, 2020
Priority date	Jul 31, 2019
Publication date	Jul 26, 2022
Grant date	Jul 26, 2022

Abstract

The present disclosure discloses an amylopectin-based cyclic glucan and the processing method for the same, and belongs to the technical field of food processing. The present disclosure uses a starch as a raw material, prepares an amylopectin-based cyclic glucan through sugar chain degradation grading and glycosidase-catalyzed trans-glycoside technology, and can be used as a steady-state carrier material for food active factors. The method of the disclosure has advantages of green environmental protection, high processing yield and low cost. The prepared product has high branching degree, special large ring structure and good water solubility, and can be used for steady-state delivery and active protection of natural functional substances, involving nutritional food, medicine, daily chemicals and other fields.

First claim

What is claimed is:

1. A method for processing an amylopectin-based cyclic glucan, comprising:
dispersing a defatted starch into a solvent to obtain a 1 g/mL to 5 g/mL starch suspension, wherein the dispersing step comprises: suspending 10 to 25 g of the defatted starch in 6 to 10 mL of absolute ethanol, then adding 10 to 100 mL of an acid catalyst solution comprising an acid catalyst to catalyze degradation of the defatted starch, and reacting at 20 to 60° C. for 30 to 120 minutes, thereby obtaining a starch degradation product;
dissolving the starch degradation product into a buffer solution,
adding a trans-glycosidase preparation to the starch degradation product,
wherein the trans-glycosidase preparation comprises a microorganism-derived trans-glycosidase obtained by fermentation of archaea or bacteria,
wherein a ratio of sugar chain branching activity to depolymerization activity of the trans-glycosidase preparation is greater than 30, and
wherein the trans-glycosidase preparation is from Calditerricolayamamurae UTM801, CGMCC 6185, Streptococcus thermophiles ATCC 14485 , Thermomus thermophiles ATCC 33923, or Aeropyrum pernix K1;
heating to inactivate the enzyme in the trans-glycosidase preparation; and
isolating the amylopectin-based cyclic glucan,
wherein a size of a cyclic structure in the amylopectin-based cyclic glucan is DP 19 to DP 50,
wherein a ratio of α-1,6 glycosidic bond in the amylopectin-based cyclic glucan is 5.0% to 7.0%, and
wherein a molecular weight of the amylopectin-based cyclic glucan is 3 kDa to 9 kDa.

2. The method according to claim 1 , wherein an amount of the trans-glycosidase preparation added in the dissolving step is: 600 to 1000 U of the trans-glycosidase preparation per 10 to 25 g of the defatted starch.

3. The method according to claim 1 , wherein in the dispersing step, the acid catalyst is prepared as an aqueous solution with a pH value of 2.5 to 4.0 for catalysis, and a volume ratio of the added amount of the acid catalyst aqueous solution to the defatted starch suspension is 10 to 100:6 to 10.

4. The method according to claim 1 , wherein in the dissolving step, the starch degradation product is dissolved in the buffer solution to prepare a solution with a mass concentration of 2% to 30%.

5. The method according to claim 1 , wherein in the dissolving step, before adding the trans-glycosidase preparation, the buffer solution in which the starch degradation product is dissolved is heated to 60 to 80° C., and then the trans-glycosidase preparation is added, maintaining the temperature and reacting for 8 to 16 hours after adding the trans-glycosidase preparation.

6. The method according to claim 1 , wherein a method for activation culture of archaea or bacteria comprises the following steps:
taking a bacterial solution stored in a glycerol tube and inoculating it into a sterilized seed LB medium for culture under sterile conditions; and
the fermentation comprises the following steps:
inoculating a seed solution after activation culture into a fermentation LB medium,
incubating in a constant temperature shaker to a bacterial concentration of OD 600 of 0.6,
centrifuging at 10,000 rpm for 15 minutes,
discarding the supernatant to collect a bacterial cell, and
obtaining the trans-glycosidase preparation by steps of lyophilization and pulverization.

7. The method of claim 1 , wherein a molecular weight of the amylopectin-based cyclic glucan is 3 kDa to 7 kDa.

8. The method of claim 1 , wherein the acid catalyst is any one or more of phosphoric acid, boric acid, sulfonic, acid, and sulfate.

9. The method of claim 1 , wherein the buffer solution is phosphate buffer.

10. The method of claim 1 , wherein the cyclic structure is composed of 6.5% α-1,6 glycosidic bonds and 93.5% α-1,4 glycosidic bonds, and the average size is DP21.

11. The method of claim 1 , wherein a yield is between 6.0% and 20.0%.

12. The method of claim 1 , wherein the acid catalyst is boric acid.

13. The method of claim 1 , wherein the acid catalyst is phosphoric acid and the trans-glycosidase preparation is from Thermomus thermophiles.

Assignees

• Univ Jiangnan

Inventors

• Miao Ming
• Jiang Bo
• Chen Chen
• Liu Yao
• Yang Yuqi
• Zhang Tao

Classifications

• C08B30/12 Primary

  Degraded, {destructured} or non-chemically modified starch {, e.g. mechanically, enzymatically or by irradiation; Bleaching of starch (preparation of chemical derivatives of starch C08B31/00)} · CPC title

• C12P19/04

  Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds · CPC title

• A23L29/225

  Farinaceous thickening agents other than isolated starch or derivatives · CPC title

• A23L29/212

  Starch; Modified starch; Starch derivatives, e.g. esters or ethers (containing starch hydrolysates, e.g. dextrin, A23L29/30) · CPC title

• C12P19/14

  produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title