Placental tissue component compositions for treatment of skin defects and methods using same

Patent metadata

Field	Value
Publication number	US-11395837-B2
Application number	US-202017106916-A
Country	US
Kind code	B2
Filing date	Nov 30, 2020
Priority date	May 18, 2018
Publication date	Jul 26, 2022
Grant date	Jul 26, 2022

Abstract

The present disclosure provides methods of treating defects such as wrinkles or scars via a subdermal injection of placental tissue components. It also provides placental tissue compositions maintained within the barrel of a syringe, which may be for use in treating such defects.

First claim

What is claimed is:

1. A method of preparing an injectable placental tissue composition, said method comprising:
a) decellularizing placental disk;
b) dehydrating the placental disk;
c) dehydrating one or more of amniotic membrane, chorionic membrane, and umbilical cord;
d) combining the dehydrated placental disk with the one or more of dehydrated amniotic membrane, chorionic membrane, and umbilical cord; and
e) milling the composition formed in step d) so as to form dehydrated particles.

2. The method of claim 1 , further comprising sifting the dehydrated particles using a sieve having a size from 50-500 μm.

3. The method of claim 2 , further comprising rehydrating the sifted, dehydrated particles so as to form a rehydrated composition.

4. The method of claim 3 , further comprising placing the rehydrated composition in a syringe.

5. The method of claim 1 , wherein the dehydrated particles are about 1-20% dehydrated amniotic membrane and/or chorionic membrane particles, if present, about 1-30% dehydrated umbilical cord particles, if present, and about 50-98% dehydrated, decellularized placental disc particles, by weight.

6. The method of claim 1 , wherein the particles comprise placental disc and amniotic membrane.

7. The method of claim 1 , wherein the particles comprise placental disc and chorionic membrane.

8. The method of claim 1 , wherein the particles comprise placental disc, amniotic membrane, and chorionic membrane.

9. The method of claim 1 , wherein the particles comprise placental disc and umbilical cord.

10. The method of claim 1 , wherein the particles comprise placental disc, amniotic membrane, chorionic membrane, and umbilical cord.

11. The method of claim 1 , wherein the particles have a size from about 50-150 μm.

Assignees

• Mimedx Group Inc

Inventors

• Levaughn Rick
• Arjunon Sivakkumar
• Taylor Olivia

Classifications

• A61L27/3804

  characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells · CPC title

• A61P17/02

  for treating wounds, ulcers, burns, scars, keloids, or the like · CPC title

• A61K9/146

  with organic macromolecular compounds · CPC title

• A61Q19/08

  Anti-ageing preparations · CPC title

• A61K35/51

  Umbilical cord; Umbilical cord blood; Umbilical stem cells · CPC title