System and method for fluid flow management in a bioprocessing system

Patent metadata

Field	Value
Publication number	US-11371007-B2
Application number	US-201916968391-A
Country	US
Kind code	B2
Filing date	Feb 8, 2019
Priority date	Feb 9, 2018
Publication date	Jun 28, 2022
Grant date	Jun 28, 2022

Abstract

A bioprocessing system includes a first fluid assembly having a first fluid assembly line connected to a first port of a first bioreactor vessel though a first bioreactor line of a first bioreactor vessel, a second fluid assembly having a second fluid assembly line connected to a second port of the first bioreactor vessel through a second bioreactor line of the first bioreactor vessel, and an interconnect line providing for fluid communication between the first fluid assembly and the second fluid assembly, and for fluid communication between the second bioreactor line of the first bioreactor vessel and the first bioreactor line of the first bioreactor vessel.

First claim

What is claimed is:

1. A bioprocessing system, comprising:
a first fluid assembly having a first fluid assembly line connected to a first port of a first bioreactor vessel though a first bioreactor line of the first bioreactor vessel, the first bioreactor line of the first bioreactor vessel including a first bioreactor line valve for providing selective fluid communication between the first fluid assembly and the first port of the first bioreactor vessel;
a second fluid assembly having a second fluid assembly line connected to a second port of the first bioreactor vessel through a second bioreactor line of the first bioreactor vessel, the second bioreactor line of the first bioreactor vessel including a second bioreactor line valve for providing selective fluid communication between the second fluid assembly and the second port of the first bioreactor vessel;
an interconnect line providing for fluid communication between the first fluid assembly and the second fluid assembly, and for fluid communication between the second bioreactor line of the first bioreactor vessel and the first bioreactor line of the first bioreactor vessel; and
a sampling assembly connected to the interconnect line intermediate the second bioreactor line valve and the first bioreactor line valve.

2. The bioprocessing system of claim 1 , wherein:
the interconnect line is connected to the second bioreactor line of the first bioreactor vessel, and the first bioreactor line and the first fluid assembly line; and
wherein the interconnect line includes an interconnect line valve.

3. The bioprocessing system of claim 2 , further comprising:
a filtration line in fluid communication with the interconnect line and the first bioreactor line of the first bioreactor vessel; and
a filter positioned along the filtration line.

4. The bioprocessing system of claim 3 , wherein:
the filtration line surrounds the interconnect line valve of the interconnect line so that a flow of fluid through the interconnect line can be forced through the filtration line.

5. The bioprocessing system of claim 4 , further comprising:
a permeate waste line providing fluid communication between the filter and a waste reservoir connected to the second fluid assembly for conveying waste removed from the fluid to the waste reservoir.

6. The bioprocessing system of claim 5 , further comprising:
a permeate waste pump configured to pump the waste from the filter to the waste reservoir.

7. The bioprocessing system of claim 3 , wherein:
the filter is one of a hollow fiber filter and a flat sheet membrane.

8. The bioprocessing system of claim 1 , wherein:
the first fluid assembly comprises a plurality tubing tails, each tubing tail of the first fluid assembly being configured for connection to one of a plurality of first reservoirs; and
the second fluid assembly includes a plurality tubing tails, each tubing tail of the second fluid assembly being configured for connection to one of a plurality of second reservoirs.

9. The bioprocessing system of claim 8 , wherein:
each tubing tail of the first fluid assembly includes a tubing tail valve for controlling a flow of fluid to or from a respective one of the plurality of first reservoirs; and
each tubing tail of the second fluid assembly includes a tubing tail valve for controlling a flow of fluid to or from a respective one of the plurality of second reservoirs.

10. The bioprocessing system of claim 1 wherein
the sampling assembly includes a sampling assembly line providing for fluid communication between the sampling assembly and the second port of the first bioreactor vessel.

11. The bioprocessing system of claim 10 , further comprising:
a sterile air source line connected to at least one of the first fluid assembly, the second fluid assembly, the interconnect line and/or the fluid flowpath intermediate the second bioreactor line valve and the first bioreactor line valve, the sterile air source line including a sterile air source line valve for selectively controlling a flow of sterile air from a sterile air source to at least one of the first fluid assembly, the second fluid assembly and/or the interconnect line.

12. The bioprocessing system of claim 1 , wherein:
the sampling assembly includes at least one tubing tail configuration for connection to a sampling terminus.

13. The bioprocessing system of claim 1 , further comprising:
a bidirectional first pump located along the first fluid assembly line and configured to initiate a flow of fluid between the first fluid assembly and the first bioreactor vessel, and between the first fluid assembly and the second fluid assembly.

14. The bioprocessing system of claim 13 , further comprising:
a bidirectional second pump configured to initiate a flow of fluid between the second fluid assembly and the first bioreactor vessel.

15. The bioreactor system of claim 14 , wherein:
the bidirectional second pump is configured to initiate a flow of fluid between the first bioreactor vessel and a second bioreactor vessel.

16. The bioprocessing system of claim 1 , wherein:
the first fluid assembly line of the first fluid assembly is further connected to a first port of a second bioreactor vessel through a first bioreactor line of the second bioreactor vessel, the first bioreactor line of the second bioreactor vessel including a first bioreactor line valve for providing selective fluid communication between the first fluid assembly and the first port of the second bioreactor vessel; and
wherein the second fluid assembly line of the second fluid assembly is connected to a second port of the second bioreactor vessel through a second bioreactor line of the second bioreactor vessel, the second bioreactor line of the second bioreactor vessel include a second bioreactor line valve for providing selective fluid communication between the second fluid assembly and the second port of the second bioreactor vessel.

17. The bioprocessing system of claim 16 , wherein:
the interconnect line provides for fluid communication between the second bioreactor line of the second bioreactor vessel and the first bioreactor line of the second bioreactor vessel.

18. The bioprocessing system of claim 17 , wherein:
the interconnect line provides for fluid communication between the second bioreactor line of the second bioreactor vessel and the second fluid assembly.

19. A bioprocessing system, comprising:
a first fluid assembly having a first fluid assembly line connected to a first port of a first bioreactor vessel though a first bioreactor line of the first bioreactor vessel, the first bioreactor line of the first bioreactor vessel including a first bioreactor line valve for providing selective fluid communication between the first fluid assembly and the first port of the first bioreactor vessel;
a second fluid assembly having a second fluid assembly line connected to a second port of the first bioreactor vessel through a second bioreactor line of the first bioreactor vessel, the second bioreactor line of the first bioreactor vessel including a second bioreactor line valve for providing selective fluid communication between the second fluid assembly and the second port of the first bioreactor vessel;
an interconnect line providing for fluid communication between the first fluid assembly and the second fluid assembly, and for fluid communication between the second bioreactor line of the first bioreactor vessel and the first bioreactor line of the first bioreactor vessel; and
a bidirectional first pump located along

Assignees

• Global Life Sciences Solutions Usa Llc

Inventors

• Griffin Weston Blaine
• Corwin Alex D
• Zhang Xiaohua
• Smith Reginald Donovan
• Liu Zhen
• Zhang Chengkun
• Keskar Vandana
• Davis Brian Michael
• Shaikh Kashan

Classifications

• C12M33/14

  with filters, sieves or membranes · CPC title

• C12M23/58

  Reaction vessels connected in series or in parallel (combinations of bioreactors with other apparatus, C12M43/00) · CPC title

• C12M47/02

  Separating microorganisms from the culture medium; Concentration of biomass (separating microorganisms from their culture media C12N1/02) · CPC title

• C12M29/00

  Means for introduction, extraction or recirculation of materials, e.g. pumps (pumps per se F04B) · CPC title

• C12M41/44 Primary

  of volume or liquid level · CPC title