Dielectrophoresis separators with cell ejection devices

Patent metadata

Field	Value
Publication number	US-11364503-B2
Application number	US-201716077362-A
Country	US
Kind code	B2
Filing date	Jul 17, 2017
Priority date	Jul 17, 2017
Publication date	Jun 21, 2022
Grant date	Jun 21, 2022

Abstract

A microfluidic device may, in an example, include at least one microfluidic channel, a dielectrophoresis separator to separate a plurality of cells passing within the at least one microfluidic channel, and a thermal resistor to eject at least one cell from the microfluidic device. A cassette may, in an example, include a die coupled to a substrate of the cassette, the die including at least one microfluidic channel, a dielectrophoresis separator along the microfluidic channel to separate a plurality of cells passing within the microfluidic channel, and an ejection device to eject at least one of the plurality of cells into an assay well.

First claim

What is claimed is:

1. A microfluidic device, comprising:
a dielectrophoresis separator;
a first plurality microfluidic channels, at least one of the first plurality configured to pass a fluid and at least one of the first plurality configured to pass a sheath fluid to the dielectrophoresis separator;
the dielectrophoresis separator fluidically coupled the first plurality of microfluidic channels and configured to separate a plurality of cells;
a number of outlets;
a second plurality of microfluidic channels fluidically coupled to the dielectrophoresis separator and configured to direct fluid exiting the dielectrophoresis separator to the number of outlets, the number of outlets are respectively fluidically coupled to the second plurality of microfluidic channels and comprise a firing chamber; and
the firing chamber comprises a thermal resistor configured to eject at least one cell of the plurality of cells, the firing chamber being fluidically coupled to one of the second plurality of microfluidic channels.

2. The microfluidic device of claim 1 , further comprising a sense electrode downstream of the dielectrophoresis separator and configured to detect a presence of at least one cell of the plurality of cells in one of the second plurality of microfluid channels.

3. The microfluidic device of claim 1 , further comprising multiple sheath fluid inlets each fluidically coupled to a respective corresponding one of the first plurality of microfluidic channels, the multiple sheath fluid inlets configured to provide a sheath fluid flow to the respective corresponding one of the first plurality of microfluidic channel channels.

4. The microfluidic device of claim 3 , further comprising a sample inlet fluidically coupled to a respective one of the first plurality of microfluidic channels, the sample inlet configured to provide a flow of cells through the respective one of the first plurality of microfluidic channel channels wherein the sample inlet is different from the multiple sheath inlets.

5. The microfluidic device of claim 1 , wherein the number of outlets comprises a waste outlet and a test cell outlet.

6. The microfluidic device of claim 1 , wherein the second plurality of microfluidic channels comprises a first microfluidic channel leading away from the dielectrophoresis separator and a second microfluidic channel leading away from the dielectrophoresis separator.

7. The microfluidic device of claim 6 , the number of outlets comprises at least two outlets;
wherein the first microfluidic channel and the second microfluidic channel leading away from the dielectrophoresis separator each leads to a respective one of the at least two outlets.

8. The microfluidic device of claim 7 , wherein one of the least two outlets comprises a test cell outlet and another one of the at least two outlets comprises an analysis outlet.

9. The microfluidic device of claim 2 , wherein the thermal resistor is configured to ejects the at least one cell in response to the at least one cell being detected as being present in one of the plurality of second microfluidic channels by the sense electrode, and the sense electrode indicates that the at least one cell of the plurality of cells is not of a type of cell to be analyzed.

10. The microfluidic device of claim 4 , wherein the multiple sheath fluid inlets and sample inlet merge in a culmination portion of the first plurality of microfluidic channels.

11. A cassette, comprising:
a substrate;
a die coupled to the substrate, the die comprising:
a sample inlet;
multiple sheath fluid inlets, different from the sample inlet;
a dielectrophoresis separator;
a first plurality of microfluidic channels respectively fluidically coupled to the sample inlet and the multiple sheath fluid inlets and respectively configured to pass a fluid and sheath fluid from the sample inlet and the multiple sheath fluid inlets to the dielectrophoresis separator;
the dielectrophoresis separator fluidically coupled to the first plurality of microfluidic channels and configured to separate a plurality of cells;
a number of outlets;
a second plurality of microfluidic channels fluidically coupled to the dielectrophoresis separator and configured to direct fluid exiting the dielectrophoresis separator to the number of outlets, the number of outlets are respectively coupled to the second plurality of microfluidic channels and comprise a firing chamber; and
the firing chamber comprises an ejection device configured to eject at least one of the plurality of cells, the firing chamber being fluidically coupled to one of the second plurality of microfluidic channels.

12. The cassette of claim 11 , further comprising a test cell outlet; wherein one of the second plurality of microfluidic channels microfluidic channels is fluidically coupled to the test cell outlet.

13. The cassette of claim 12 , wherein each of the second plurality of microfluidic channels includes a sense electrode to sense a cell within each of the second plurality of microfluidic channels.

14. The cassette of claim 12 , further comprising a waste outlet; wherein one of the second plurality of microfluidic channels is fluidically coupled to the waste outlet.

15. The cassette of claim 12 , further comprising an analysis outlet; wherein one of the second plurality of microfluidic channels is fluidically coupled to the analysis outlet.

16. A fluid ejection system comprising:
the cassette of claim 11 , further comprising a number of electrical connection pads defined on a surface of the cassette; and
an electrical dispensing device connected to the number of electrical connection pads.

17. The fluid ejection system of claim 16 , wherein the electrical dispensing device provides a voltage to the dielectrophoresis separator via the number of electrical connection pads.

18. A method, comprising:
passing a sample including a plurality of cells from a sample inlet to a first one of a first plurality of microfluidic channels that is fluidically coupled to the sample inlet; wherein each of the first plurality of microfluidic channels is fluidically coupled to a dielectrophoresis separator;
passing a sheath fluid from a first sheath fluid inlet and a second sheath fluid inlet to a second and third of the first plurality of microfluidic channels that are fluidically coupled to the first sheath fluid inlet and second sheath fluid inlet respectively and are fluidically coupled to the dielectrophoresis separator;
merging the sample and the sheath fluid from the respective first plurality of microfluidic channels before reaching the dielectrophoresis separator;
providing the merged sample and sheath fluid to the dielectrophoresis separator;
creating an electrical field in the dielectrophoresis separator configured to separate the plurality of cells by type;
directing from the dielectrophoresis separator a first type of cell of the plurality of cells to a firing chamber that comprises a thermal resistor via a first one of a second plurality of microfluidic channels coupled to the dielectrophoresis separator;
directing from the dielectrophoresis separator a second type of cell of the plurality of cells to a waste outlet via a second one of the second plurality of microfluidic channels; and
directing from the dielectrophoresis separator a third type of cell of the plurality of cells to an analysis outlet via a third one of the second plurality of microfluidic channels.

19. The method of claim 18 , wherein the first sheath fluid inlet is disposed on a first side of the sample i

Assignees

• Hewlett Packard Development Co

Inventors

• Shkolnikov Viktor
• Ward Kenneth

Classifications

• B03C5/005

  Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength · CPC title

• B01L2300/0816

  Cards, e.g. flat sample carriers usually with flow in two horizontal directions · CPC title

• G01N33/49

  Blood {(chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80)} · CPC title

• B01L2200/0652

  Sorting or classification of particles or molecules · CPC title

• B01L3/502761 Primary

  specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules · CPC title