Microbial production of fatty diols

Patent metadata

Field	Value
Publication number	US-11359216-B2
Application number	US-202016889211-A
Country	US
Kind code	B2
Filing date	Jun 1, 2020
Priority date	Jul 18, 2014
Publication date	Jun 14, 2022
Grant date	Jun 14, 2022

Abstract

The disclosure relates to fatty diols and recombinant microorganisms for producing them. More particularly, the disclosure relates to recombinant microorganisms engineered to produce fatty diols via fermentation. Further encompassed is a process that uses the microorganisms to produce fatty diols from a simple carbon source.

First claim

We claim:

1. A method for producing a 1,3 fatty diol comprising:
(i) culturing a recombinant microorganism in a fermentation broth that comprises a simple carbon source, wherein said recombinant microorganism expresses;
a nucleic acid sequences encoding a thioesterase having the classification EC 3.1.2.-, EC 3.1.1.5, or EC 3.1.2.14, wherein the thioesterase uses 3-hydroxyacyl-ACP as a substrate; and
a nucleic acid sequence encoding a carboxylic acid reductase (CAR) having the classification EC 1.2.99.6; and

(ii) isolating the 1.3 fatty diol from said fermentation broth.

2. The method of claim 1 , wherein said 1,3 fatty diol is produced in vivo.

3. The method of claim 1 , wherein said 1,3 fatty diol is selected from the group consisting of a C 5 1,3 fatty diol, a C 6 1,3 fatty diol, a C 7 1,3 fatty diol, a C 8 1,3 fatty diol, a C 9 1,3 fatty diol, a C 10 1,3 fatty diol, a C 11 1,3 fatty diol, a C 12 1,3 fatty diol, a C 13 1,3 fatty diol, a C 14 1,3 fatty diol, a C 15 1,3 fatty diol, a C 16 1,3 fatty diol, a C 17 1,3 fatty diol, a C 18 1,3 fatty diol, and a C 19 1,3 fatty diol.

4. The method of claim 1 , wherein the recombinant microorganism further expresses a nucleic acid sequence encoding an alcohol dehydrogenase having the classification EC 1.1.1.-.

5. The method of claim 1 , wherein said simple carbon source is derived from a renewable feedstock.

6. The method of claim 1 , wherein:
the thioesterase is FatB1; and
the carboxylic acid reductase (CAR) is selected from the group consisting of CarB from Mycobacterium smegmatis , CarA from Mycobacterium smegmatis , FadD9 from Mycobacterium smegmatis , CAR from Mycobacterium genavense , CAR from Nocardia iowensis , and CAR from Nocardia brasiliensis.

7. The method of claim 4 , wherein said alcohol dehydrogenase is alrA.

8. The method of claim 1 , wherein the recombinant microorganism is a recombinant bacterium.

9. The method of claim 8 , wherein the recombinant bacterium is Escherichia coli ( E. coli ).

Assignees

• Genomatica Inc

Inventors

• Schirmer Andreas W
• Helman Noah
• Wang Haibo
• Hu Zhihao
• Arlagadda Vikranth
• Ramos-Solis Alma Itzel
• Clarke Elizabeth

Classifications

• C12N9/93

  Ligases (6) · CPC title

• C12N9/18

  Carboxylic ester hydrolases {(3.1.1)} · CPC title

• C12Y301/02

  Thioester hydrolases (3.1.2) · CPC title

• C12Y602/01003

  Long-chain-fatty-acid-CoA ligase (6.2.1.3) · CPC title

• C12Y301/01005

  Lysophospholipase (3.1.1.5) · CPC title