Method for improving resistance to breakage and eggshell strength

Patent metadata

Field	Value
Publication number	US-11358996-B2
Application number	US-201916711821-A
Country	US
Kind code	B2
Filing date	Dec 12, 2019
Priority date	Aug 12, 2016
Publication date	Jun 14, 2022
Grant date	Jun 14, 2022

Abstract

Described herein are FGF-23 epitope peptides, methods of producing antibodies in laying hens by injecting the peptides, and methods of improving resistance to eggshell breakage and/or increasing eggshell strength by administering an FGF-23 epitope peptide to a laying hen.

First claim

The invention claimed is:

1. A method of improving resistance to egg shell breakage and/or improving egg shell strength, comprising administering to a laying hen greater than 37 weeks in age an effective amount of an FGF-23 epitope peptide to improve resistance to egg shell breakage and/or improve the egg shell strength of eggs laid by the hen, wherein the FGF-23 epitope peptide comprises SEQ ID NO:3, SEQ ID NO:4, or SEQ ID NO:9, and wherein the FGF-23 epitope peptide is conjugated to a carrier protein.

2. The method of claim 1 , further comprising administering to the laying hen a booster administration one or more weeks or more after the first administration, wherein the booster administration comprises an effective amount of an FGF-23 epitope peptide to improve resistance to egg shell breakage and/or improve the egg shell strength of eggs laid by the hen, wherein the FGF-23 epitope peptide comprises SEQ ID NO:3, SEQ ID NO:4, or SEQ ID NO:9, and wherein the FGF-23 epitope peptide is conjugated to a carrier protein.

3. The method of claim 2 , wherein the carrier protein is bovine gamma globulin, bovine serum albumin, keyhole limpet hemocyanin, or ovalbumin.

4. The method of claim 1 , wherein the laying hen is a chicken, a turkey, a duck, a goose, a peafowl, an emu, a pheasant, a guinea fowl, or a quail.

5. The method of claim 1 , wherein administering is oral, aerosol, or parenteral administration.

6. The method of claim 1 , wherein improved resistance to egg shell breakage and/or improved egg shell strength is determine by one or more of:
load resistance testing with a 50-kg load cell, 2 mm/s test speed, 0.001 kg trigger force, and a 50 mm diameter cylindrical probe;
egg specific gravity measured by flotation in sodium chloride and water solutions having sodium chloride concentrations of 1.070, 1.074, 1.078, 1.082, 1.086, 1.090, 1.094, and 1.098M; and
shell weight.

Assignees

• Wisconsin Alumni Res Found

Inventors

• Butz Daniel
• Ren Zhouzheng
• Piepenburg Alexis Jo
• Cook Mark Eric

Classifications

• A61K39/0005

  Vertebrate antigens (from snakes A61K39/38) · CPC title

• A61K38/1825

  Fibroblast growth factor [FGF] · CPC title

• A61K2039/62

  characterised by the link between antigen and carrier · CPC title

• A61K47/64

  Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent (peptidic linkers A61K47/65) · CPC title

• C07K16/22

  against growth factors {; against growth regulators} · CPC title