Sensor system, method and cross-linked hydrogel for detecting the presence or concentration of analytes

Patent metadata

Field	Value
Publication number	US-11350855-B2
Application number	US-201715831983-A
Country	US
Kind code	B2
Filing date	Dec 5, 2017
Priority date	Dec 5, 2016
Publication date	Jun 7, 2022
Grant date	Jun 7, 2022

Abstract

A sensor system detects a presence or concentration of an analyte in a medium. The sensor system contains a sensor having a sensor head with a chamber. The sensor head has a permeable area through which the analyte can pass into the chamber when the sensor head contacts the medium. A cross-linked hydrogel fills the chamber, the hydrogel is configured to undergo a change in volume when contacting the analyte passed into the chamber which leads to a change in pressure in the chamber. A pressure sensor is configured to measure the pressure in the chamber for detecting the presence or concentration of the analyte.

First claim

The invention claimed is:

1. A sensor system for detecting a presence or concentration of an analyte in a medium, the sensor system comprising:
a sensor having a sensor head with a chamber, said sensor head having a permeable area through which the analyte can pass into said chamber when said sensor head contacts the medium;
a cross-linked hydrogel filling said chamber, said cross-linked hydrogel being configured to undergo a change in volume when contacting the analyte passed into said chamber which leads to a change in pressure in said chamber, said cross-linked hydrogel having a plurality of a responsive component, said responsive component being responsive to the analyte such that the volume of said cross-linked hydrogel decreases when said responsive component binds to the analyte such that the pressure in said chamber decreases, and the volume of said cross-linked hydrogel increases when the analyte is released from said responsive component such that the pressure in said chamber increases, said responsive component comprising a crown ether; and
a pressure sensor configured to measure the pressure in said chamber for detecting the presence or the concentration of the analyte, wherein said cross-linked hydrogel is in isochoric condition in said chamber and has a generally linear dependency of a measured pressure for temperatures of said cross-linked hydrogel below a lower critical solution temperature,
wherein molecules of said crown ether are attached to said cross-linked hydrogel such that they form pairs of said crown ether molecules, wherein two of said crown ethers of a respective pair of molecules are configured to bind the analyte simultaneously, and wherein the analyte is disposed between said crown ethers of the respective pair of molecules in a bound state.

2. The sensor system according to claim 1 , wherein said cross-linked hydrogel comprises at least one of:
a temperature coefficient KT=Δp/ΔT being a slope of a Pressure-Temperature-curve of said cross-linked hydrogel, the temperature coefficient KT lying within a range of 200 mbar/K to 600 mbar/K; and
a hydrogel coefficient KHG=KK+/KT in a range from 0.001 to 0.2.

3. The sensor system according to claim 1 , wherein said cross-linked hydrogel comprises a polymer of one of a monomer selected from the group consisting of N-isopropylmethacrylamide, N-ethyl-N-methyl acrylamide, N,N-diethyl acrylamide, N,N-dimethylaminoethyl methacrylate, and (ethylenglycol)methacrylate.

4. The sensor system according to claim 1 , wherein said cross-linked hydrogel has a network with a plurality of cross-linked polymer chains and a plurality of non-cross-linked polymer chains bound to said network, and only said non-cross-linked polymer chains contain the responsive component.

5. The sensor system according to claim 1 , wherein said sensor contains an analyzer configured to determine the presence or the concentration of the analyte using a measured pressure.

6. The sensor system according to claim 5 , wherein said sensor contains a telemeter configured to transfer the measured pressure to said analyzer.

7. The sensor system according to claim 1 , further comprising:
at least one reference sensor having a reference hydrogel for reducing cross-sensitivity of said sensor, said reference sensor further having a reference sensor head with a reference chamber filled with said reference hydrogel and a reference pressure sensor for measuring a reference pressure in said reference chamber of said reference sensor; and
an analyzer configured to correct a pressure measured in said chamber of said sensor with or by subtracting the reference pressure.

8. The sensor system according to claim 1 , wherein said sensor contains a component which mechanically decouples a change in the pressure of said cross-linked hydrogel in said chamber resulting from a change in temperature of said cross-linked hydrogel from an analyte concentration dependent pressure measured by said pressure sensor of said sensor.

9. The sensor system according to claim 1 , wherein said responsive component is configured to detect K+ as the analyte.

10. The sensor system according to claim 1 , wherein said responsive component achieves a linear dependency between temperature and pressure irrespective if the analyte is present or not.

11. The sensor system according to claim 1 , wherein said cross-linked hydrogel contains a Poly(N-isopropylacrylamid) having at least 20 mol-% of said crown ether chemically bonded to polymer chains.

12. The sensor system according to claim 1 , wherein said crown ether is 15-crown-5.

Assignees

• Biotronik Se & Co Kg

Inventors

• Bunge Andreas
• Ebert Henning
• Skerl Olaf
• Borck Alexander

Classifications

• A61B5/14546

  for measuring analytes not otherwise provided for, e.g. ions, cytochromes · CPC title

• A61B2560/0247

  for compensation or correction of the measured physiological value · CPC title

• G01N7/10

  by allowing diffusion of components through a porous wall and measuring a pressure or volume difference · CPC title

• A61B2560/0223

  of calibration, e.g. protocols for calibrating sensors · CPC title

• G01N15/06

  Investigating concentration of particle suspensions (by weighing G01N5/00; investigating sedimentation of particle suspensions G01N15/04; investigating individual particles G01N15/10) · CPC title