Detector with reduced fluorescence range noise

Patent metadata

Field	Value
Publication number	US-11333604-B2
Application number	US-202016948524-A
Country	US
Kind code	B2
Filing date	Sep 22, 2020
Priority date	Dec 28, 2017
Publication date	May 17, 2022
Grant date	May 17, 2022

Abstract

There is set forth herein a device comprising structure defining a detector surface configured for supporting biological or chemical substances, and a sensor array comprising light sensors and circuitry to transmit data signals using photons detected by the light sensors. The device can include one or more features for reducing fluorescence range noise in a detection band of the sensor array.

First claim

The invention claimed is:

1. A device comprising:
a detector surface configured for supporting biological or chemical samples;
a sensor array comprising light sensors and light guides;
wherein the light guides receive excitation light and emissions signal light from the detector surface, wherein the light guides extend toward respective light sensors of the sensor array and comprise filter material, and wherein the filter material comprises dye having a photon emission quencher;
wherein the dye comprises metal complex dye suspended in a polymer binder matrix.

2. The device of claim 1 , wherein the dye comprises a weight concentration ratio of metal complex dye to polymer binder in the range of from about 70:30 to about 90:10.

3. The device of claim 1 , wherein the detector surface includes a reaction recess for supporting a sample, wherein the reaction recess comprises an index of refraction and dimension to cancel background radiation in a detection band of the sensor array.

4. The device of claim 1 , wherein the dye comprises a transition metal complex dye.

5. The device of claim 1 , wherein the dye comprises a transition metal and wherein the transition metal has an absorption spectral profile overlapping a fluorescence emissions spectral profile of the dye.

6. The device of claim 1 , wherein the dye comprises a metal complex dye that reduces an auto-fluorescence of the filter material.

7. The device of claim 1 , wherein the filter material comprises a counter ion associated to a metal complex dye.

8. The device of claim 7 , wherein the counter ion comprises an alkyl amine having at least one hydrocarbon group of at least four carbon atoms.

9. A method comprising:
depositing filter material within guide cavities of a guide cavity array that are aligned with and disposed above respective light sensors of a light sensor array, wherein the filter material comprises a metal complex dye; and
fabricating a structure defining a detector surface for supporting biological or chemical samples above cavities of the guide cavity array and light sensors of the light sensor array.

10. The method of claim 9 , wherein the depositing filter material comprises using chemical vapor deposition, and wherein subsequent to the depositing, the deposited filter material is subject to processing using one or more of etching and planarizing.

11. The method of claim 9 , wherein the filter material comprises a homogeneous matrix of metal complex dye and polymer binder.

12. The method of claim 9 , wherein the filter material comprises a matrix of metal complex dye and polymer binder and wherein a weight concentration of metal complex dye to polymer binder is in the range of from about 70:30 to about 90:10.

13. The method of claim 9 , wherein the fabricating a structure defining a detector surface comprises forming a reaction recess defined in the detector surface, wherein the forming includes configuring the reaction recess so that based on an index of refraction of the detector surface and a dimensional characteristic of the reaction recess, an induced electromagnetic field radiating from the detector surface cancels background light energy incident on the detector surface in a detection wavelength band of the light sensor array.

14. The method of claim 9 , wherein the method includes subjecting one or more test sample detectors to testing to determine information relating a dimension of a detector surface to an electromagnetic field cancellation effect and wherein the fabricating the structure defining a detector surface includes dimensioning, using the determined information, a reaction recess of the detector surface to cancel light energy incident on the detector surface in a detection band of the light sensor array.

15. The method of claim 9 , wherein the filter material comprises a counter ion associated to the metal complex dye.

16. The method of claim 15 , wherein the counter ion comprises an alkyl amine having at least one hydrocarbon group of at least four carbon atoms.

17. A method comprising:
fabricating a structure defining a detector surface for supporting biological or chemical samples above cavities of a guide cavity array and light sensors of a light sensor array, wherein the fabricating a structure defining a detector surface comprises forming a reaction recess defined in the detector surface so that based on an index of refraction and a dimensional characteristic of the reaction recess, an induced electromagnetic field radiating from the detector surface cancels background light energy incident on the detector surface in a detection wavelength band of the light sensor array.

18. The method of claim 17 , wherein the reaction recess comprises an index of refraction and a dimension sufficient to transmit a center wavelength of excitation light.

19. The method of claim 17 , wherein the index of refraction and the dimension characteristic if the reaction recess are sufficient to establish a critical wavelength λc so that λc is within a range of wavelengths of between about λa and about λb, wherein wavelengths shorter than λc are transmitted by the reaction recess and wherein wavelengths longer than λc are cancelled by the reaction recess, wherein λa is the center wavelength of excitation light and wherein λb is the shortest detection band wavelength of the sensor array.

Assignees

• Illumina Inc

Inventors

• Fung Tracy H
• Sabounchi Poorya
• Hirschbein Bernard
• Pinto Joseph
• Khurana Tarun
• Smith Randall
• Feng Wenyi

Classifications

• G01N21/6486 Primary

  Measuring fluorescence of biological material, e.g. DNA, RNA, cells (G01N21/6428 takes precedence) · CPC title

• G01N21/6402

  Atomic fluorescence; Laser induced fluorescence · CPC title

• G01N21/01

  Arrangements or apparatus for facilitating the optical investigation · CPC title

• H10F39/18

  Complementary metal-oxide-semiconductor [CMOS] image sensors; Photodiode array image sensors · CPC title

• H10F39/8053

  Colour filters · CPC title