Compact multi-color flow cytometer having compact detection module

Patent metadata

Field	Value
Publication number	US-11333597-B2
Application number	US-201815942430-A
Country	US
Kind code	B2
Filing date	Mar 30, 2018
Priority date	Apr 26, 2016
Publication date	May 17, 2022
Grant date	May 17, 2022

Abstract

A system, an apparatus, and a method are provided for a modular flow cytometer with a compact size. In one embodiment, the modular flow cytometry system includes the following: a laser system for emitting laser beams; a flow cell assembly positioned to receive the laser beams at an interrogation region of a fluidics stream where fluoresced cells scatter the laser beams into fluorescent light; a fiber assembly positioned to collect the fluorescent light; and a compact light detection module including a first image array having a transparent block, a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and a plurality of filters in a row coupled to a second side of the transparent block opposite the first side.

First claim

What is claimed is:

1. A flow cytometry system, comprising:
a laser system for emitting laser beams;
a flow cell assembly positioned to receive the laser beams at an interrogation region of a fluidics stream where fluoresced cells scatter the laser beams into fluorescent light;
a fiber assembly positioned to collect the fluorescent light; and
a light detection module including
a first image array having
a transparent block,
a plurality of micro-mirrors in a row coupled to a first side of the transparent block,
a plurality of filters in a row coupled to a second side of the transparent block opposite the first side, and
a first plurality of detector channels in a row forming a first detector chain adjacent the first image array.

2. The flow cytometry system of claim 1 , wherein:
the laser system, the flow cell assembly, and the compact light detection module are coupled to an optical plate assembly of the flow cytometry system.

3. The flow cytometry system of claim 1 , wherein
each of the plurality of filters reflects light to one of the plurality of micro-mirrors and passes light of a differing wavelength range and each of the plurality of micro-mirrors reflects light to one of the plurality of filters, such that incident light into the image array zigzags back and forth between consecutive filters of the plurality of filters and consecutive micro-mirrors of the plurality of micro-mirrors, and
a radius of curvature of each of the plurality of micro-mirrors directs light onto even filters and reimages light onto odd filters of the plurality of filters along the row.

4. The flow cytometer system of claim 3 , wherein
the first plurality of detector channels respectively are in light communication with the plurality of filters of the first image array, wherein each detector channel includes a focusing lens, and a photo detector.

5. The flow cytometer system of claim 4 , wherein
the light detection module further includes an input channel that images from an aperture at a fiber to a surface of a first filter of the first plurality of filters; and
the flow cytometer system further comprises
a collection lens with a first side facing a sample chamber and an opposing second side, the first side of the collection lens having a first numeric aperture (NA) and the second side of the collection lens having a second NA less than the first NA; and
an optical fiber with a first end aligned with an optical axis of the collection lens and a second end aligned with an optical axis of the input channel of the light detection module, the optical fiber having a fiber NA greater than or equal to the second NA of the collection lens.

6. The flow cytometer system of claim 5 , wherein
the input channel magnifies the size of the input image size from the aperture to the surface of the first filter.

7. The flow cytometer system of claim 5 , wherein
the input channel includes a collimating lens to receive launched fluorescent light and collimate the light, a blocking filter to reject a laser light used to stimulate fluorochromes in the collimated light, and a focusing lens to focus the collimated light from the collimating lens onto the first filter.

8. The flow cytometer system of claim 3 , wherein
the light detection module further includes
a second image array having
a transparent block,
a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and
a plurality of filters in a row coupled to a second side of the transparent block opposite the first side.

9. The flow cytometer system of claim 8 , wherein
the light detection module further includes
a second plurality of detector channels in a row forming a second detector chain adjacent the second image array, the second plurality of detector channels respectively in light communication with the plurality of filters of the second image array, wherein each detector channel includes a focusing lens, and a photo detector.

10. The flow cytometer system of claim 9 , wherein
the light detection module further includes an input channel that couples an input image from an aperture at a fiber to a surface of a first filter of the first plurality of filters and a surface of a first filter of the second plurality of filters; and
the flow cytometer system further comprises
a collection lens with a first side facing a sample chamber and an opposing second side, the first side of the collection lens having a first numeric aperture (NA) and the second side of the collection lens having a second NA less than the first NA; and
an optical fiber with a first end aligned with an optical axis of the collection lens and a second end aligned with an optical axis of the input channel of the light detection module, the optical fiber having a fiber NA greater than or equal to the second NA of the collection lens.

11. The flow cytometer system of claim 10 , wherein
the input channel magnifies the size of the input image size from the aperture to the surface of the first filter of each of the first and second plurality of filters.

12. The flow cytometer system of claim 10 , wherein
the input channel includes
a collimating lens to receive launched fluorescent light and collimate the light,
a blocking filter to reject a laser light used to stimulate fluorochromes in the collimated light,
a beam splitter to split the collimated light into a first range of wavelengths and a second range of wavelengths different from the first range,
a first focusing lens to focus the collimated light with the first range of wavelengths onto the first filter of the first plurality of filters, and
a second focusing lens to focus the collimated light with the second range of wavelengths onto the first filter of the second plurality of filters.

13. The flow cytometer system of claim 3 , wherein
a radius of curvature of the plurality of micro-mirrors forms a focal length equal to a beam path length from micro-mirror to opposing filter.

14. The flow cytometer system of claim 3 , wherein
each of the plurality of filters is a dichroic filter.

15. The flow cytometer system of claim 3 , wherein
each of the plurality of filters is a bandpass filter.

16. The flow cytometer system of claim 3 , wherein
each of the plurality of filters are a combination of a dichroic filter and a bandpass filter coupled in light communication.

17. The flow cytometer system of claim 3 , wherein
wherein the light directed by each of the plurality of micro-mirrors is collimated onto even filters.

18. A modular flow cytometry system, comprising:
a modular enclosure with a compact footprint configured to rest onto a bench top;
an optical plate assembly coupled to a top of the modular enclosure;
a laser system with a plurality of excitation lasers coupled to the optical plate to emit a plurality of laser beams;
a flow cell assembly coupled to the optical plate assembly, the flow cell assembly positioned to receive the plurality of laser beams at an interrogation region of a fluidics stream where fluoresced cells scatter the plurality of laser beams into fluorescent light;
at least one fiber assembly having at least one fiber head at one end coupled to the optical plate assembly, the least one fiber head having an input end of at least one collection fiber positioned to collect the fluorescent light; and
at least one light detection module coupled to the modular enclosure, the at least one light detection module including
a first image array having
a transparent block,
a plurality of micro-mirrors

Assignees

• Cytek Biosciences Inc

Inventors

• Yan Ming
• Hsieh Yung-Chieh
• Vrane David
• Chase Eric
• Jiang Wenbin

Classifications

• G01J3/4406

  Fluorescence spectrometry · CPC title

• G01N21/53

  within a flowing fluid, e.g. smoke · CPC title

• G01N15/1434

  Optical arrangements · CPC title

• G01J3/0291

  Housings; Spectrometer accessories; Spatial arrangement of elements, e.g. folded path arrangements · CPC title

• G01J3/021

  using plane or convex mirrors, parallel phase plates, or particular reflectors · CPC title