Method for applications of nano-sized materials as cell growth regulators / cell growth enhancers for plant, and agent including nano-sized materials

Patent metadata

Field	Value
Publication number	US-11326145-B2
Application number	US-201816013311-A
Country	US
Kind code	B2
Filing date	Jun 20, 2018
Priority date	Feb 20, 2013
Publication date	May 10, 2022
Grant date	May 10, 2022

Abstract

One aspect of the disclosure is directed to a method for activation/enhancement of cell growth of a plant. The method also stimulates the production of pharmaceutically active metabolites, including alkaloids, in plant cell cultures. The method includes providing a nano-sized material contained agent, and treating the plant with the nano-sized material contained agent to allow sufficient interaction of cells of the plant with the nano-sized material so as to activate/enhance the cell growth of the plant or to stimulate the production of pharmaceutically active metabolites.

First claim

What is claimed is:

1. A method for production of alkaloid in Catharanthus cell culture, comprising:
providing a cell culture having one or more cells of a Catharanthus plant;
introducing an amount of a nano-sized material into the cell culture to form a mixture thereof;
maintaining the mixture at a temperature for a period of time to allow sufficient interaction of the one or more cells with the nano-sized material so as to enhance the production of the alkaloid in the cell culture; and
extracting the alkaloid from the cell culture,
wherein the nano-sized material comprises at least multi-walled carbon nanotubes having a purity of about 98% and a diameter of about 20 nm; and
wherein the nano-sized material has a concentration in a range of 5-100 g/mL in the cell culture.

2. The method of claim 1 , wherein the step of providing the cell culture further comprising:
germinating seeds of the plant in a Murashige-Skoog (MS) medium to establish calli of the plant; and
maintaining the callus culture at a predetermined condition to produce the cell culture having the one or more cells of the plant.

3. The method of claim 1 , wherein the alkaloid includes vinblastine and/or vincristine.

4. The method of claim 1 , further comprising maintaining the nano-sized material and the cell culture mixture in the light cultivation condition.

5. The method of claim 1 , wherein the temperature is in a range of 5−35° C., and the period of time is in a range from 0.1 hours to 2 months.

6. The method of claim 1 , wherein the amount of the nano-sized material has a concentration in a range of 25-100 μg/mL in the cell culture.

7. A method for enhancing production of an alkaloid in a Catharanthus cell culture, comprising:
providing a cell culture having one or more cells of a Catharanthus plant;
introducing an amount of a nano-sized material into the cell culture to form a mixture thereof; and
maintaining the mixture at a temperature for a period of time to allow sufficient interaction of the one or more cells with the nano-sized material so as to enhance the production of the alkaloid in the cell culture,
wherein the nano-sized material comprises multi-walled carbon nanotubes having a purity of about 98% and a diameter of about 20 nm; and
wherein the nano-sized material has a concentration in a range of 5-100 g/mL in the cell culture.

8. The method of claim 7 , wherein the amount of the nano-sized material has a concentration in a range of 25-100 μg/mL in the cell culture.

9. The method of claim 7 , further comprising maintaining the nano-sized material and the cell culture mixture in the light cultivation condition.

10. The method of claim 7 , wherein the temperature is in a range of 5−35° C., and the period of time is in a range from 0.1 hours to 2 months.

Assignees

• Univ Arkansas

Inventors

• Khodakovskaya Mariya
• Mcgehee Diamond

Classifications

• A01H3/00 Primary

  Processes for modifying phenotypes {, e.g. symbiosis with bacteria} (A01H4/00 takes precedence) · CPC title

• C12N5/04 Primary

  Plant cells or tissues {(culture media C12N5/0025)} · CPC title

• A01H4/001

  Culture apparatus for tissue culture · CPC title

• A01H4/002

  Culture media for tissue culture · CPC title