Methods for detecting estrone by mass spectrometry

Patent metadata

Field	Value
Publication number	US-11280798-B2
Application number	US-201916579438-A
Country	US
Kind code	B2
Filing date	Sep 23, 2019
Priority date	Dec 13, 2007
Publication date	Mar 22, 2022
Grant date	Mar 22, 2022

Abstract

Provided are methods for determining the amount of estrone in a sample using mass spectrometry. The methods generally involve ionizing estrone in a sample and detecting and quantifying the amount of the ion to determine the amount of estrone in the sample.

First claim

That which is claimed is:

1. A method for determining the amount of estrone in a sample, said method comprising:
(a) adding deuterated estrone to a sample;
(b) derivatizing estrone to produce a derivative of estrone;
(c) purifying the sample by high performance liquid chromatography (HPLC);
(d) ionizing the derivative of estrone and deuterated estrone in said sample to produce one or more ions detectable by mass spectrometry; and
(e) detecting the amount of the ions by tandem mass spectrometry.

2. The method of claim 1 , wherein said deuterated estrone is d 4 -estrone.

3. The method of claim 1 , wherein said deuterated estrone is 2,4,16,16-d 4 estrone.

4. The method of claim 1 , wherein said method further comprises subjecting estrone from said sample to an extraction.

5. The method of claim 1 , wherein said method has a limit of quantitation less than or equal to 20 pg/mL.

6. The method of claim 1 , wherein said method has a limit of detection less than or equal to 10 pg/mL.

7. The method of claim 1 , wherein said sample is a human plasma or serum sample.

8. The method of claim 1 , wherein said ionization is atmospheric pressure chemical ionization (APCI) or electrospray ionization (ESI).

9. A method for determining the amount of estrone in a sample, said method comprising:
(a) adding deuterated estrone to a sample;
(b) derivatizing estrone to produce a derivative of estrone;
(c) purifying the sample by liquid chromatography;
(d) ionizing derivatized estrone and deuterated estrone in said sample to produce one or more ions detectable by mass spectrometry; and
(e) detecting the amount of the ions by tandem mass spectrometry.

10. The method of claim 9 , wherein said liquid chromatography comprises high turbulence liquid chromatography (HTLC).

11. The method of claim 9 , wherein said liquid chromatography comprises high performance liquid chromatography (HPLC).

12. The method of claim 9 , wherein said deuterated estrone is d 4 -estrone.

13. The method of claim 9 , wherein said deuterated estrone is 2,4,16,16-d 4 estrone.

14. The method of claim 9 , wherein said method further comprises subjecting estrone from said sample to an extraction.

15. The method of claim 9 , wherein said method has a limit of quantitation less than or equal to 20 pg/mL.

16. The method of claim 9 , wherein said method has a limit of detection less than or equal to 10 pg/mL.

17. The method of claim 9 , wherein said sample is a human plasma or serum sample.

18. The method of claim 9 , wherein said ionization is atmospheric pressure chemical ionization (APCI) or electrospray ionization (ESI).

Assignees

• Quest Diagnostics Invest Inc

Inventors

• Goldman Mildred M
• Reitz Richard E

Classifications

• Y10T436/24

  Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry · CPC title

• Y10T436/203332

  Hydroxyl containing · CPC title

• G01N33/743 Primary

  Steroid hormones · CPC title

• Y10T436/20

  Oxygen containing · CPC title

• G01N27/628

  and a beam of energy, e.g. laser enhanced ionisation · CPC title