Methods for detecting estrone by mass spectrometry

Patent metadata

Field	Value
Publication number	US-9678087-B2
Application number	US-201414533602-A
Country	US
Kind code	B2
Filing date	Nov 5, 2014
Priority date	Dec 13, 2007
Publication date	Jun 13, 2017
Grant date	Jun 13, 2017

Abstract

Provided are methods for determining the amount of estrone in a sample using mass spectrometry. The methods generally involve ionizing estrone in a sample and detecting and quantifying the amount of the ion to determine the amount of estrone in the sample.

First claim

That which is claimed is:

1. A method for determining the amount of estrone in a human plasma or serum sample, said method comprising:
(a) adding deuterated estrone to a human plasma or serum sample;
(b) processing the sample by high performance liquid chromatography (HPLC);
(c) ionizing estrone and deuterated estrone in said processed sample with electrospray ionization (ESI) in positive ion mode to produce one or more ions detectable by mass spectrometry; and
(d) detecting the amount of the ions by tandem mass spectrometry;
(e) comparing the amount of said one or more ions of estrone and said one or more ions of deuterated estrone to determine the amount of estrone in the sample;
wherein said method has a limit of quantitation less than or equal to 500 pg/mL.

2. The method of claim 1 , wherein said estrone is derivatized prior to ionization.

3. The method of claim 1 , wherein said deuterated estrone is d 4 -estrone.

4. The method of claim 1 , wherein said deuterated estrone is 2,4,16,16-d 4 estrone.

5. The method of claim 1 , wherein said method further comprises subjecting estrone from said plasma or serum sample to an extraction.

6. The method of claim 1 , wherein said method has a limit of quantitation less than or equal to 20 pg/mL.

7. The method of claim 1 , wherein said method has a limit of detection less than or equal to 10 pg/mL.

8. A method for determining the amount of estrone in a human plasma or serum sample, said method comprising:
(a) adding deuterated estrone to a human plasma or serum sample;
(b) processing the sample by liquid chromatography;
(c) ionizing estrone and deuterated estrone in said processed sample with electrospray ionization (ESI) in positive ion mode to produce one or more ions detectable by mass spectrometry; and
(d) detecting the amount of the ions by tandem mass spectrometry;
(e) comparing the amount of said one or more ions of estrone and said one or more ions of deuterated estrone to determine the amount of estrone in the sample;
wherein said method has a limit of quantitation less than or equal to 500 pg/mL.

9. The method of claim 8 , wherein said estrone is derivatized prior to ionization.

10. The method of claim 8 , wherein said liquid chromatography comprises high turbulence liquid chromatography (HTLC).

11. The method of claim 8 , wherein said liquid chromatography comprises high performance liquid chromatography (HPLC).

12. The method of claim 8 , wherein said deuterated estrone is d 4 -estrone.

13. The method of claim 8 , wherein said deuterated estrone is 2,4,16,16-d 4 estrone.

14. The method of claim 8 , wherein said method further comprises subjecting estrone from said plasma or serum sample to an extraction.

15. The method of claim 8 , wherein said method has a limit of quantitation less than or equal to 20 pg/mL.

16. The method of claim 8 , wherein said method has a limit of detection less than or equal to 10 pg/mL.

17. A method for determining the amount of estrone in a human plasma or serum sample, said method comprising:
(a) adding deuterated estrone to a human plasma or serum sample;
(b) ionizing estrone and deuterated estrone in said processed sample with electrospray ionization (ESI) in positive ion mode to produce one or more ions detectable by mass spectrometry; and
(c) detecting the amount of the ions by tandem mass spectrometry;
(d) comparing the amount of said one or more ions of estrone and said one or more ions of deuterated estrone to determine the amount of estrone in the sample;
wherein said method has a limit of quantitation less than or equal to 500 pg/mL.

18. The method of claim 17 , wherein said estrone is derivatized prior to ionization.

19. The method of claim 17 , wherein said deuterated estrone is d 4 -estrone.

20. The method of claim 17 , wherein said deuterated estrone is 2,4,16,16-d 4 estrone.

21. The method of claim 17 , wherein said method further comprises subjecting estrone from said plasma or serum sample to an extraction.

22. The method of claim 17 , wherein said method has a limit of quantitation less than or equal to 20 pg/mL.

23. The method of claim 17 , wherein said method has a limit of detection less than or equal to 10 pg/mL.

Assignees

• Quest Diagnostics Invest Inc
• Quest Diagnostics Invest Inc

Inventors

• Goldman Mildred M
• Reitz Richard E

Classifications

• G01N27/64

  using wave or particle radiation to ionise a gas, e.g. in an ionisation chamber · CPC title

• Y10T436/200833

  Carbonyl, ether, aldehyde or ketone containing · CPC title

• Y10T436/20

  Oxygen containing · CPC title

• Y10T436/25

  including sample preparation · CPC title

• G01N33/6851

  Methods of protein analysis involving laser desorption ionisation mass spectrometry · CPC title