Bioreaction container

Patent metadata

Field	Value
Publication number	US-11261417-B2
Application number	US-201716462637-A
Country	US
Kind code	B2
Filing date	Nov 7, 2017
Priority date	Nov 23, 2016
Publication date	Mar 1, 2022
Grant date	Mar 1, 2022

Abstract

A bioreaction container including a culture chamber configured to contain a culture solution and a life form in an inner space, the culture chamber having an open upper end; a chamber cover portion coupled to the upper end of the culture chamber, the chamber cover portion having a protruding tube provided on one side thereof so as to communicate with the inner space; a filter cap coupled to the protruding tube in an attachable/detachable manner so as to open/close the protruding tube; a gas introduction portion configured to penetrate the chamber cover portion and to communicate with the inner space so as to supply a predetermined gas into the inner space; and an acidity/basicity adjustment portion installed on the chamber cover portion while containing an adjustment solution that adjusts pH of the culture solution such that the adjustment solution is discharged into the inner space by pneumatic pressure.

First claim

What is claimed is:

1. A bioreaction container comprising:
a culture chamber configured to contain a culture solution and a life form in an inner space, the culture chamber having an open upper end;
a chamber cover portion coupled to the upper end of the culture chamber, the chamber cover portion having a protruding tube provided on one side thereof so as to communicate with the inner space;
a filter cap coupled to the protruding tube in an attachable/detachable manner so as to open/close the protruding tube;
a gas introduction portion configured to penetrate the chamber cover portion and to communicate with the inner space so as to supply a predetermined gas into the inner space; and
an acidity/basicity adjustment portion installed on the chamber cover portion while containing an adjustment solution that adjusts pH of the culture solution such that the adjustment solution is discharged into the inner space by pneumatic pressure, wherein
acidity/basicity adjustment portion comprises:
a storage tank having a containing space in which the adjustment solution is stored, the storage tank being mounted on the chamber cover portion so as to surround a surface of the protruding tube;
an air introduction tube configured to introduce air supplied from outside to the storage tank; and
an outflow tube configured such that the adjustment solution is introduced into the inner space by pneumatic pressure provided through the air introduction tube,
wherein the chamber cover portion has a protruding side wall formed to protrude in a direction of the protruding tube upward along an outer peripheral surface thereof and has an annular mounting space provided between the protruding side wall and the protruding tube, and the storage tank is installed in the mounting space.

2. The bioreaction container of claim 1 , wherein the storage tank comprises a first storage tank in which the adjustment solution that is acidic is stored and a second storage tank in which the adjustment solution that is basic is stored; and air introduction tube and the outflow tube are formed so as to correspond to each of the first storage tank and the second storage tank.

3. The bioreaction container of claim 1 , wherein the storage tank is formed in an arc-shaped barrel structure so as to be fitted/fixed within the annular mounting space along a circumferential direction of the chamber cover portion such that there is no mounting interference with the gas introduction portion in the annular mounting space.

4. The bioreaction container of claim 1 , wherein the storage tank comprises a first storage tank in which the adjustment solution that is acidic is stored and a second storage tank in which the adjustment solution that is basic is stored; and the storage tank is formed in an arc-shaped barrel structure so as to be fitted/fixed to the annular mounting space along a circumferential direction of the chamber cover portion such that there is no mounting interference with the gas introduction portion in the annular mounting space.

5. The bioreaction container of claim 1 , wherein the acidity/basicity adjustment portion comprises:
a storage tank having a containing space in which the adjustment solution is stored, the storage tank being mounted on the chamber cover portion so as to surround a surface of the protruding tube;
a suction tube configured to penetrate/be coupled to the storage tank such that one end thereof is immersed in the adjustment solution;
an air flow tube having one end configured to penetrate/be coupled to the chamber cover portion such that air supplied from the other end thereof flows into the inner space; and
a discharge tube provided such that the other end of the suction tube and one side of the air flow tube communicate with each other.

6. The bioreaction container of claim 1 , wherein the acidity/basicity adjustment portion comprises:
a storage tank having a containing space in which the adjustment solution is stored, the storage tank being mounted on the chamber cover portion so as to surround a surface of the protruding tube;
an air introduction tube having one end configured to receive air and having the other end configured to penetrate/be coupled to the storage tank;
an immersed tube having one end configured to communicate with the other end of the air introduction tube, the immersed tube being immersed in the adjustment solution in a bending shape, a microhole being formed in the immersed tube such that the adjustment solution flows therein; and
an outflow tube having one end configured to communicate with the other end of the immersed tube and having the other end configured to penetrate/be coupled to the chamber cover portion.

7. The bioreaction container of claim 1 , wherein the filter cap comprises:
a cap body configured as a tube body having a cavity formed therein and coupled to the protruding tube in an attachable/detachable manner; and
a filtering filter coupled to one side of an inner peripheral surface of the cavity to be able to rotate downward while being supported elastically such that external alien substances moving into the cavity are filtered out, and air is allowed to move in/out.

8. The bioreaction container of claim 1 , wherein the gas introduction portion comprises:
a first gas introduction tube configured to supply oxygen into the inner space; and
a second gas introduction tube configured to supply carbon dioxide or nitrogen into the inner space.

9. The bioreaction container of claim 1 , wherein at least one selected from a DO measurement sensor configured to measure the amount of dissolved oxygen in the culture solution, a pH measurement sensor configured to measure acidity/basicity of the culture solution, and a temperature sensor configured to measure the temperature of the culture solution, is provided on one side of the culture chamber.

10. The bioreaction container of claim 1 , wherein a barrier-type baffle having a through-hole formed therein is mounted in the inner space of the culture chamber so as to facilitate mixing between the culture solution and the life form, or a protruding-type baffle is formed so as to protrude from an inner surface of the culture chamber toward the inner space along a longitudinal direction of the culture chamber.

11. The bioreaction container of claim 1 , wherein the storage tank is mounted on the chamber cover portion such that a bottom of the containing space in which the adjustment solution is stored is above a top of the inner space of the culture chamber.

12. The bioreaction container of claim 1 , further comprising an immersed tube having a first end connected to an end of the air introduction tube to receive the air introduced into the storage tank by the air introduction tube and a second end connected to an end of the outflow tube to deliver the adjustment solution by the pneumatic pressure to the outflow tube.

Assignees

• Spl Co Ltd
• E Cell Cd Ltd
• Univ Sejong Ind Acad Coop Foud
• Industry Academy Cooperation Foundation Of Sejona Univ

Inventors

• Heo Sang Oh
• Kim Dong Hoon
• Kim Doo Hyun
• Oh Duk Jae

Classifications

• B01F31/22

  with supporting means moving in a horizontal plane, e.g. describing an orbital path for moving the containers about an axis which intersects the receptacle axis at an angle · CPC title

• C12M41/26 Primary

  of pH · CPC title

• C12M41/40

  of pressure · CPC title

• C12M23/38

  Caps; Covers; Plugs; Pouring means · CPC title

• C12M37/02

  Filters · CPC title