Electrophoresis device and electrophoresis method

Patent metadata

Field	Value
Publication number	US-11226307-B2
Application number	US-202016885828-A
Country	US
Kind code	B2
Filing date	May 28, 2020
Priority date	Mar 27, 2015
Publication date	Jan 18, 2022
Grant date	Jan 18, 2022

Abstract

Provided is an electrophoresis device that, by electrophoresis, feeds a sample into capillaries and optically detects the sample, the electrophoresis device being provided with capillaries, a capillary head provided at the distal end of the capillaries, a phoretic medium-filled container used for electrophoresis and filled with a phoretic medium, a guide member that covers the side surface of the phoretic medium-filled container, a seal member that seals from below the phoretic medium filled in the phoretic medium-filled container, and a plunger that presses the seal member.

First claim

The invention claimed is:

1. An electrophoresis device comprising:
capillaries;
a capillary head that is provided at a distal end of the capillaries;
a phoretic medium container that is used for electrophoresis and filled with a phoretic medium;
a liquid feeding mechanism that is provided with a plunger and feeds the phoretic medium in the phoretic medium container to the capillaries; and
a flat plate that holds the phoretic medium container;
wherein the phoretic medium container is provided with a syringe, a seal member that seals the phoretic medium in the syringe from below and is operated within the syringe by pressing with the plunger, and an elastic body that seals an upper part of the syringe, and
wherein the capillary head is inserted into the phoretic medium container and presses the elastic body,
an anode side buffer solution container mounted on the flat plate;
a cathode side buffer solution container mounted on the flat plate;
a sample container mounted on the flat plate;
a first driving unit that drives the flat plate in a first direction parallel to a surface of the flat plate; and
a second driving unit that drives the flat plate in a second direction perpendicular to the surface of the flat plate;
wherein the cathode side buffer solution container and the sample container are disposed in parallel in the first direction,
wherein the cathode side buffer solution container is provided with a waste liquid vessel, a cathode side cleaning vessel, and a buffer solution vessel for cathode side electrophoresis,
wherein the waste liquid vessel, the cathode side cleaning vessel, and the buffer solution vessel for cathode side electrophoresis are sequentially partitioned and provided in the first direction, and
wherein the phoretic medium container and the waste liquid vessel are disposed in parallel in a third direction orthogonal to the first direction on the surface of the flat plate.

2. The electrophoresis device according to claim 1 ,
wherein a distal end of the capillary head has an acute angle.

3. The electrophoresis device according to claim 1 ,
wherein the capillary head has a capillary head distal end and a capillary head boss which outer diameter is greater than that of the capillary head distal end, and the elastic body is pressed by the capillary head boss.

4. The electrophoresis device according to claim 1 ,
wherein the capillary head can move relative to the phoretic medium container and is releasably connected to the phoretic medium container.

5. The electrophoresis device according to claim 1 , further comprising:
a third driving unit that drives the sample container in a third direction orthogonal to the first direction on the surface of the flat plate.

6. The electrophoresis device according to claim 1 ,
wherein the seal member has a greater expansion factor than that of the syringe.

7. The electrophoresis device according to claim 1 ,
wherein the capillary head is integrated with an electrode of an anode side.

8. The electrophoresis device according to claim 1 , further comprising:
a contact position detecting unit that detects a position where the plunger is in contact with the seal member.

9. An electrophoresis device comprising:
capillaries;
a capillary head that is provided at a distal end of the capillaries;
a phoretic medium container that is used for electrophoresis and filled with a phoretic medium;
a liquid feeding mechanism that is provided with a plunger and feeds the phoretic medium in the phoretic medium container to the capillaries;
a flat plate that holds the phoretic medium container;
wherein the phoretic medium container is provided with a syringe, a seal member that seals the phoretic medium in the syringe from below and is operated within the syringe by pressing with the plunger, and an elastic body that seals an upper part of the syringe; and
wherein the capillary head is inserted into the phoretic medium container and presses the elastic body;
an anode side buffer solution container mounted on the flat plate;
a cathode side buffer solution container mounted on the flat plate;
a sample container mounted on the flat plate;
a first driving unit that drives the flat plate in a first direction parallel to a surface of the flat plate; and
a second driving unit that drives the flat plate in a second direction perpendicular to the surface of the flat plate;
wherein the phoretic medium container and the anode side buffer solution container are disposed in parallel in the first direction,
wherein the anode buffer solution container is provided with an anode side cleaning vessel, a buffer solution vessel for anode side electrophoresis, and a buffer solution vessel for anode side sample introduction,
wherein the anode side cleaning vessel, the buffer solution vessel for anode side electrophoresis, and the buffer solution vessel for anode side sample introduction are sequentially partitioned and provided in the first direction, and
wherein the buffer solution vessel for anode side sample introduction and the sample container are disposed in parallel in a third direction orthogonal to the first direction on the surface of the flat plate.

10. The electrophoresis device according to claim 1 ,
wherein the seal member is a recessed shape.

Assignees

• Hitachi High Tech Corp

Inventors

• Kimura Ryusuke
• Yamazaki Motohiro
• Miyata Hitoshi
• Higashino Hiroyuki

Classifications

• G01N27/44721

  by optical means · CPC title

• G01N27/44782

  of a plurality of samples · CPC title

• G01N27/44756

  Apparatus specially adapted therefor · CPC title

• G01N27/447 Primary

  using electrophoresis · CPC title

• G01N27/44743 Primary

  Introducing samples · CPC title