Electrophoresis device and electrophoresis method

Patent metadata

Field	Value
Publication number	US-10705046-B2
Application number	US-201515560395-A
Country	US
Kind code	B2
Filing date	Mar 27, 2015
Priority date	Mar 27, 2015
Publication date	Jul 7, 2020
Grant date	Jul 7, 2020

Abstract

Provided is an electrophoresis device that, by electrophoresis, feeds a sample into capillaries and optically detects the sample, the electrophoresis device being provided with capillaries, a capillary head provided at the distal end of the capillaries, a phoretic medium-filled container used for electrophoresis and filled with a phoretic medium, a guide member that covers the side surface of the phoretic medium-filled container, a seal member that seals from below the phoretic medium filled in the phoretic medium-filled container, and a plunger that presses the seal member.

First claim

The invention claimed is:

1. An electrophoresis device which feeds a sample into capillaries by electrophoresis and optically detects the sample, the device comprising:
capillaries;
a capillary head that is provided at a distal end of the capillaries;
a phoretic medium-filled container that is used for the electrophoresis and filled with a phoretic medium;
a guide member that covers a side surface of the phoretic medium-filled container;
a seal member that seals the phoretic medium filled in the phoretic medium-filled container from below; and
a plunger that presses the seal member,
wherein, the capillary head is disposed inside the phoretic medium-filled container.

2. The electrophoresis device according to claim 1 ,
wherein an upper part of the phoretic medium-filled container is an opening portion, and the opening portion is sealed with an elastic body.

3. The electrophoresis device according to claim 1 ,
wherein a distal end of the capillary head has an acute angle.

4. The electrophoresis device according to claim 1 , further comprising:
a flat plate that holds the phoretic medium-filled container.

5. The electrophoresis device according to claim 4 , wherein, on the flat plate, an anode side buffer solution container, a cathode side buffer solution container, and a sample container are further mounted.

6. The electrophoresis device according to claim 5 , further comprising:
a first driving unit that drives the flat plate in a first direction parallel to a surface of the flat plate; and
a second driving unit that drives the flat plate in a second direction perpendicular to the surface of the flat plate.

7. The electrophoresis device according to claim 6 ,
wherein the phoretic medium-filled container and an anode buffer solution container are disposed in parallel in the first direction.

8. The electrophoresis device according to claim 6 ,
wherein the cathode side buffer solution container and the sample container are disposed in parallel in the first direction.

9. The electrophoresis device according to claim 8 ,
wherein the cathode side buffer solution container is provided with a waste liquid layer, a cathode side cleaning layer, and a buffer solution layer for cathode side electrophoresis,
wherein the waste liquid layer, the cathode side cleaning layer, and the buffer solution layer for cathode side electrophoresis are sequentially partitioned and provided in the first direction, and
wherein the phoretic medium-filled container and the waste liquid layer are disposed in parallel in a third direction orthogonal to the first direction on the surface of the flat plate.

10. The electrophoresis device according to claim 1 , further comprising:
a third driving unit that drives the sample container in the third direction orthogonal to the first direction on the surface of the flat plate.

11. The electrophoresis device according to claim 1 ,
wherein the seal member has a greater expansion factor than that of the phoretic medium-filled container.

12. The electrophoresis device according to claim 1 ,
wherein the capillary head is integrated with an electrode of an anode side.

13. The electrophoresis device according to claim 1 , further comprising:
a contact position detecting unit that detects a position where the plunger is in contact with the seal member.

14. The electrophoresis device according to claim 7 ,
wherein the anode buffer solution container is provided with the anode side cleaning layer, the buffer solution layer for anode side electrophoresis, and the buffer solution layer for anode side sample introduction,
wherein the anode side cleaning layer, the buffer solution layer for anode side electrophoresis, and the buffer solution layer for anode side sample introduction are sequentially partitioned and provided in the first direction, and
wherein the buffer solution layer for anode side sample introduction and the sample container are disposed in parallel in the third direction orthogonal to the first direction on the surface of the flat plate.

15. The electrophoresis device according to claim 1 ,
wherein the seal member is a U-shaped.

16. The electrophoresis device according to claim 1 , wherein the capillary head is connected to the phoretic medium-filled container via a rubber stopper disposed at an upper end of the phoretic medium-filled container.

17. The electrophoresis device according to claim 1 , wherein the capillary head is releasably connected to the phoretic medium container.

Assignees

• Hitachi High Tech Corp

Inventors

• Kimura Ryusuke
• Yamazaki Motohiro
• Miyata Hitoshi
• Higashino Hiroyuki

Classifications

• G01N27/44743 Primary

  Introducing samples · CPC title

• G01N27/44721

  by optical means · CPC title

• G01N27/44782

  of a plurality of samples · CPC title

• G01N27/44791

  Microapparatus (sample containers with integrated microfluidic structures B01L3/5027) · CPC title

• G01N27/447 Primary

  using electrophoresis · CPC title