Methods for producing corn plants with northern leaf blight resistance and compositions thereof

Patent metadata

Field	Value
Publication number	US-11219174-B2
Application number	US-202016877649-A
Country	US
Kind code	B2
Filing date	May 19, 2020
Priority date	Dec 18, 2015
Publication date	Jan 11, 2022
Grant date	Jan 11, 2022

Abstract

The present disclosure is in the field of plant breeding and disease resistance. The disclosure provides methods for breeding corn plants having northern leaf blight (NLB) resistance using marker-assisted selection. The disclosure further provides corn germplasm resistant to NLB. The disclosure also provides markers associated with NLB resistance loci for introgressing these loci into elite germplasm in a breeding program, thus producing novel NLB resistant germplasm.

First claim

What is claimed is:

1. A method of creating a population of Northern Leaf Blight (NLB) resistant corn plants, corn seeds, or corn cells, said method comprising:
a) crossing a first NLB resistant corn plant comprising NLB resistance quantitative trait locus (QTL) with a second corn plant to generate a first population of corn plants, corn seeds, or corn cells;
b) genotyping said first population of corn plants, corn seeds, or corn cells at one or more marker loci linked to, and within 10 centimorgans (cM) of, said NLB resistance QTL NLB_5.01; and
c) selecting from said first population one or more corn plants, corn seeds, or corn cells having resistance to NLB comprising said one or more of said marker loci linked to said NLB resistance QTL NLB_5.01 linked to an NLB resistance allele selected from the group consisting of:
SEQ ID NO: 53 comprising a C or an A, at position number 62;
SEQ ID NO: 54 comprising a T, at position number 167;
SEQ ID NO: 55 comprising a C, at position number 99;
SEQ ID NO: 56 comprising a C, at position number 390;
SEQ ID NO: 57 comprising a C or an A, at position number 279;
SEQ ID NO: 58 comprising an A, at position number 101;
SEQ ID NO: 59 comprising a T or a C, at position number 61;
SEQ ID NO: 60 comprising an A, at position number 339;
SEQ ID NO: 61 comprising an A, at position number 125;
SEQ ID NO: 62 comprising a T, at position number 101;
SEQ ID NO: 63 comprising a T, at position number 369;
SEQ ID NO: 64 comprising a C or a T, at position number 101;
SEQ ID NO: 65 comprising a G, at position number 101;
SEQ ID NO: 446 comprising a T, at position number 101;
SEQ ID NO: 447 comprising a T, at position number 101;
SEQ ID NO: 448 comprising a C, at position number 216;
SEQ ID NO: 449 comprising a T, at position number 184;
SEQ ID NO: 450 comprising a C, at position number 101;
SEQ ID NO: 451 comprising a G, at position number 101;
SEQ ID NO: 452 comprising a G, at position number 265;
SEQ ID NO: 453 comprising an A, at position number 101;
SEQ ID NO: 454 comprising a T, at position number 105;
SEQ ID NO: 455 comprising a T, at position number 254;
SEQ ID NO: 456 comprising a C, at position number 322;
SEQ ID NO: 457 comprising a G, at position number 345;
SEQ ID NO: 458 comprising a G, at position number 58;
SEQ ID NO: 459 comprising a T, at position number 342;
SEQ ID NO: 460 comprising a G, at position number 542;
SEQ ID NO: 461 comprising a G, at position number 101;
SEQ ID NO: 462 comprising an A, at position number 73;
SEQ ID NO: 463 comprising a G, at position number 129;
SEQ ID NO: 464 comprising a T, at position number 101;
SEQ ID NO: 465 comprising a G, at position number 101;
SEQ ID NO: 466 comprising a C, at position number 234;
SEQ ID NO: 467 comprising an A, at position number 101; and
SEQ ID NO: 468 comprising a C, at position number 486.

2. The method of claim 1 , wherein said one or more marker loci are located in a chromosomal interval flanked by any two of SEQ ID NOs: 53 to 65 and 446 to 468, and said NLB resistance allele is at said QTL NLB_5.01.

3. The method of claim 1 , wherein said one or more marker loci are located in a chromosomal interval flanked by any two of SEQ ID NOs: 57 to 62 and 458 to 466, and said NLB resistance allele is at said QTL NLB_5.01.

4. The method of claim 1 , wherein said one or more marker loci are located in a chromosomal interval flanked by any two of SEQ ID NOs: 57 to 64 and 446 to 468, and said NLB resistance allele is at said QTL NLB_5.01.

5. The method of claim 1 , wherein said one or more marker loci are linked to, and within 5 cM of, any one of the marker loci selected from the group consisting of SEQ ID NOs: 53 to 65 and 446 to 468.

6. The method of claim 1 , wherein said NLB resistance QTL NLB_5.01 provides mild resistance or resistance to infection by Exserohilum turcicum.

7. The method of claim 1 , wherein a second population of corn plants comprising said one or more of said marker loci exhibit reduced root rot, reduced stalk rot, fewer leaf lesions, less foliage destruction, or any combination thereof, compared to corn plants lacking said NLB resistance QTL NLB_5.01 when grown under a high NLB stress condition.

8. The method of claim 1 , wherein said NLB resistance QTL NLB_5.01 does not confer a yield penalty when grown under a low NLB stress condition.

9. The method of claim 1 , wherein said method further comprises:
producing from said one or more corn plants, corn seeds, or corn cells selected in step c) a second population of corn plants, corn seeds, or corn cells comprising said NLB resistance QTL NLB_5.01.

10. The method of claim 9 , wherein said second population of corn plants or seeds exhibits a reduction of NLB rating score of 1 or more as compared to corn plants or seeds lacking said NLB resistance QTL NLB_5.01 when grown under a high NLB stress condition.

11. The method of claim 9 , wherein said second population of corn plants or seeds exhibits a reduction of NLB rating score of 2 or more as compared to corn plants or seeds lacking said NLB resistance QTL NLB_5.01 when grown under a high NLB stress condition.

12. The method of claim 1 , wherein said one or more marker loci is selected from the group consisting of:
SEQ ID NO: 53 comprising a C or an A, at position number 62;
SEQ ID NO: 54 comprising a T, at position number 167;
SEQ ID NO: 55 comprising a C, at position number 99;
SEQ ID NO: 56 comprising a C, at position number 390;
SEQ ID NO: 57 comprising a C or an A, at position number 279;
SEQ ID NO: 58 comprising an A, at position number 101;
SEQ ID NO: 59 comprising a T or a C, at position number 61;
SEQ ID NO: 60 comprising an A, at position number 339;
SEQ ID NO: 61 comprising an A, at position number 125;
SEQ ID NO: 62 comprising a T, at position number 101;
SEQ ID NO: 63 comprising a T, at position number 369;
SEQ ID NO: 64 comprising a C or a T, at position number 101;
SEQ ID NO: 65 comprising a G, at position number 101;
SEQ ID NO: 446 comprising a T, at position number 101;
SEQ ID NO: 447 comprising a T, at position number 101;
SEQ ID NO: 448 comprising a C, at position number 216;
SEQ ID NO: 449 comprising a T, at position number 184;
SEQ ID NO: 450 comprising a C, at position number 101;
SEQ ID NO: 451 comprising a G, at position number 101;
SEQ ID NO: 452 comprising a G, at position number 265;
SEQ ID NO: 453 comprising an A, at position number 101;
SEQ ID NO: 454 comprising a T, at position number 105;
SEQ ID NO: 455 comprising a T, at position number 254;
SEQ ID NO: 456 comprising a C, at position number 322;
SEQ ID NO: 457 comprising a G, at position number 345;
SEQ ID NO: 458 comprising a G, at position number 58;
SEQ ID NO: 459 comprising a T, at position number 342;
SEQ ID NO: 460 comprising a G, at position number 542;
SEQ ID NO: 461 comprising a G, at position number 101;
SEQ ID NO: 462 comprising an A, at position number 73;
SEQ ID NO: 463 comprising a G, at position number 129;
SEQ ID NO: 464 comprising a T, at position number 101;
SEQ ID NO: 465 comprising a G, at position number 101;
SEQ ID NO: 466 comprising a C, at position number 234;
SEQ ID NO: 467 comprising an A, at position number 101; and
SEQ ID NO: 468 comprising a C, at position number 486.

13. A method of creating a population of Northern Leaf Blight (NLB) resistant corn plants, corn seeds, or corn cells, said method comprising:
a) crossing a first NLB resistant corn plant comprising an NLB resistance quantitative trait loci (QTL) NLB_5.01 with a second corn plant to generate a first population of corn plants, corn seeds, or corn cells;

Assignees

• Monsanto Technology Llc

Inventors

• Bourdoncle William J
• Chopin Franck J
• Fouquet Romain
• Giovanini Marcelo P
• Maloney Peter V
• Pan Yule

Classifications

• A01H1/045

  using molecular markers · CPC title

• C12Q1/6895

  for plants, fungi or algae · CPC title

• A01H6/4684 Primary

  Zea mays [maize] · CPC title

• C12Q2600/13

  Plant traits · CPC title

• C12Q2600/156

  Polymorphic or mutational markers · CPC title