Methods for producing corn plants with northern leaf blight resistance and compositions thereof

Patent metadata

Field	Value
Publication number	US-10694693-B2
Application number	US-201615382229-A
Country	US
Kind code	B2
Filing date	Dec 16, 2016
Priority date	Dec 18, 2015
Publication date	Jun 30, 2020
Grant date	Jun 30, 2020

Abstract

The present disclosure is in the field of plant breeding and disease resistance. The disclosure provides methods for breeding corn plants having northern leaf blight (NLB) resistance using marker-assisted selection. The disclosure further provides corn germplasm resistant to NLB. The disclosure also provides markers associated with NLB resistance loci for introgressing these loci into elite germplasm in a breeding program, thus producing novel NLB resistant germplasm.

First claim

What is claimed is:

1. A method of creating a population of Northern Leaf Blight (NLB) resistant corn plants, corn seeds, or corn cells, said method comprising:
a) crossing a first NLB resistant corn plant comprising NLB resistance quantitative trait locus (QTL) NLB_4.01 with a second corn plant to generate a first population of corn plants, corn seeds, or corn cells;
b) genotyping said first population of corn plants, corn seeds, or corn cells at one or more marker loci linked to, and within 10 centimorgans (cM) of, said NLB resistance QTL NLB_4.01; and
c) selecting from said first population one or more corn plants, corn seeds, or corn cells having resistance to NLB comprising said one or more of said marker loci linked to said NLB resistance QTL NLB_4.01 linked to an NLB resistance allele selected from the group consisting of:
SEQ ID NO: 33 comprising a G, at position number 319;
SEQ ID NO: 35 comprising a G, at position number 101;
SEQ ID NO: 36 comprising an A, at position number 373;
SEQ ID NO: 37 comprising an A, at position number 115;
SEQ ID NO: 38 comprising a G, at position number 171;
SEQ ID NO: 39 comprising a T, at position number 37;
SEQ ID NO: 40 comprising a C, at position number 101;
SEQ ID NO: 41 comprising a G, at position number 101;
SEQ ID NO: 42 comprising a T, at position number 101;
SEQ ID NO: 43 comprising a T, at position number 101;
SEQ ID NO: 44 comprising a G or a T, at position number 2239;
SEQ ID NO: 45 comprising an A, at position number 569;
SEQ ID NO: 46 comprising a T, at position number 101;
SEQ ID NO: 47 comprising a C, at position number 240;
SEQ ID NO: 48 comprising a C, at position number 247;
SEQ ID NO: 49 comprising an A, at position number 719;
SEQ ID NO: 50 comprising an A, at position number 429;
SEQ ID NO: 51 comprising an A, at position number 101;
SEQ ID NO: 52 comprising a C, at position number 81;
SEQ ID NO: 471 comprising a G, at position number 173;
SEQ ID NO: 472 comprising a T, at position number 101;
SEQ ID NO: 473 comprising a G, at position number 101;
SEQ ID NO: 474 comprising a G, at position number 101; and
SEQ ID NO: 475 comprising an A, at position number 101.

2. The method of claim 1 , wherein said one or more marker loci are located in a chromosomal interval flanked by
any two of SEQ ID NOs: 32 to 52 and 471 to 475, and said NLB resistance allele is at said QTL NLB_4.01.

3. The method of claim 1 , wherein said one or more marker loci are located in a chromosomal interval flanked by
any two of SEQ ID NOs: 37 to 42 and 474, and said NLB resistance allele is at said QTL NLB_4.01.

4. The method of claim 1 , wherein said one or more marker loci are located in a chromosomal interval flanked by
any two of SEQ ID NOs: 33 to 42, 473, and 474, and said NLB resistance allele is at said QTL NLB_4.01.

5. The method of claim 1 , wherein said one or more marker loci are linked to, and within 5 cM of, any one of the marker loci selected from the group consisting of SEQ ID NOs: 32 to 52 and 471 to 475.

6. The method of claim 1 , wherein said NLB resistance QTL NLB_4.01 provides mild resistance or resistance to infection by Exserohilum turcicum.

7. The method of claim 1 , wherein a second population of corn plants comprising said one or more of said marker loci exhibit reduced root rot, reduced stalk rot, fewer leaf lesions, less foliage destruction, or any combination thereof, compared to corn plants lacking said NLB resistance QTL NLB_4.01 when grown under a high NLB stress condition.

8. The method of claim 1 , wherein said NLB resistance QTL NLB_4.01 does not confer a yield penalty when grown under a low NLB stress condition.

9. The method of claim 1 , wherein said method further comprises:
producing from said one or more corn plants, corn seeds, or corn cells selected in step c) a second population of corn plants, corn seeds, or corn cells comprising said NLB resistance QTL NLB_4.01.

10. The method of claim 9 , wherein said second population of corn plants or seeds exhibits a reduction of NLB rating score of 1 or more as compared to corn plants or seeds lacking said NLB resistance QTL NLB_4.01 when grown under a high NLB stress condition.

11. The method of claim 9 , wherein said second population of corn plants or seeds exhibits a reduction of NLB rating score of 2 or more as compared to corn plants or seeds lacking said NLB resistance QTL NLB_4.01 when grown under a high NLB stress condition.

12. The method of claim 1 , wherein said one or more marker loci is selected from the group consisting of:
SEQ ID NO: 33 comprising a G, at position number 319;
SEQ ID NO: 35 comprising a G, at position number 101;
SEQ ID NO: 36 comprising an A, at position number 373;
SEQ ID NO: 37 comprising an A, at position number 115;
SEQ ID NO: 38 comprising a G, at position number 171;
SEQ ID NO: 39 comprising a T, at position number 37;
SEQ ID NO: 40 comprising a C, at position number 101;
SEQ ID NO: 41 comprising a G, at position number 101;
SEQ ID NO: 42 comprising a T, at position number 101;
SEQ ID NO: 43 comprising a T, at position number 101;
SEQ ID NO: 44 comprising a G or a T, at position number 2239;
SEQ ID NO: 45 comprising an A, at position number 569;
SEQ ID NO: 46 comprising a T, at position number 101;
SEQ ID NO: 47 comprising a C, at position number 240;
SEQ ID NO: 48 comprising a C, at position number 247;
SEQ ID NO: 49 comprising an A, at position number 719;
SEQ ID NO: 50 comprising an A, at position number 429;
SEQ ID NO: 51 comprising an A, at position number 101;
SEQ ID NO: 52 comprising a C, at position number 81;
SEQ ID NO: 471 comprising a G, at position number 173;
SEQ ID NO: 472 comprising a T, at position number 101;
SEQ ID NO: 473 comprising a G, at position number 101;
SEQ ID NO: 474 comprising a G, at position number 101; and
SEQ ID NO: 475 comprising an A, at position number 101.

13. A method of creating a population of Northern Leaf Blight (NLB) resistant corn plants, corn seeds, or corn cells, said method comprising:
a) crossing a first NLB resistant corn plant comprising an NLB resistance quantitative trait loci (QTL) with a second corn plant to generate a first population of corn plants, corn seeds, or corn cells;
b) genotyping said first population of corn plants, corn seeds, or corn cells at one or more marker loci linked to, and within 10 centimorgans (cM) of, any one of the marker loci selected from the group consisting of SEQ ID NOs: 32 to 52 and 471 to 475, wherein said marker is associated with said NLB resistance QTL NLB_4.01; and
c) selecting from said first population one or more corn plants, corn seeds, or corn cells having resistance to NLB comprising said one or more of said marker loci linked to said NLB resistance QTL NLB_4.01 linked to an NLB resistance allele selected from the group consisting of:
SEQ ID NO: 33 comprising a G, at position number 319;
SEQ ID NO: 35 comprising a G, at position number 101;
SEQ ID NO: 36 comprising an A, at position number 373;
SEQ ID NO: 37 comprising an A, at position number 115;
SEQ ID NO: 38 comprising a G, at position number 171;
SEQ ID NO: 39 comprising a T, at position number 37;
SEQ ID NO: 40 comprising a C, at position number 101;
SEQ ID NO: 41 comprising a G, at position number 101;
SEQ ID NO: 42 comprising a T, at position number 101;
SEQ ID NO: 43 comprising a T, at position number 101;
SEQ ID NO: 44 comprising a G or a T, at position number 2239;
SEQ ID NO: 45 comprising an A, at position number 569;
SEQ ID NO: 46 comprising a T, at position number 101;
SEQ ID NO: 47 comprising a C, at position number 240;
SEQ ID

Assignees

• Monsanto Technology Llc

Inventors

• Bourdoncle William J
• Chopin Franck J
• Fouquet Romain
• Giovanini Marcelo P
• Maloney Peter V
• Pan Yule

Classifications

• A01H1/045

  using molecular markers · CPC title

• A01H6/4684 Primary

  Zea mays [maize] · CPC title

• A01H5/10 Primary

  Seeds · CPC title

• C12Q2600/156

  Polymorphic or mutational markers · CPC title

• C12Q1/6895

  for plants, fungi or algae · CPC title